UCSF home page UCSF home page About UCSF Search UCSF UCSF Medical Center
Equipment Computing ALS BL 8.3.1 Web Links BI219 Mini-Course Surveys
New Users Advanced Users Fluorescence Scans Room Temperature Shots FAQs Data Backup/Sendhome

Room Temperature Shots

James has a nice Kapton tape mount that you can press your greased cover slip onto, thus shooting the drop with absolutely minimal manipulations to the crystal. It's very nice and complemented by the fact that the camera looks down the direction of the beam, so what you see is what you get.  If you are using this, which should be supervised by James or someone who's done it before the first couple times, you CANNOT and MUST NOT rotate around phi when the collimator set up is up and in position as it WILL bang into the collimator and stuff.  You can rotate phi and get the crystal in focus with the collimator set up down and out of the way, make sure you are back at phi=0 (and the cover slip is therefore normal to the vector of the beam by this definition) before bringing up the collimator stage.  And then remember, once again...do not rotate around phi and make sure all snap shots are set to take pictures at phi=0, or if you really must, +/- ~10 degress.  Really, I wouldn't go trying it based solely on what I've written here.  These are just reminders if you've done it before and know to what all this is referring.

600 16th St, San Francisco, California, 94158-2140 | phone (415)476-8288 | fax (415) 476-1902
University of California, San Francisco || About UCSF || Search UCSF || Macromolecular Structure Group || UCSF Biochemistry