Elucidating the Basic Principles of Macromolecular & Supramolecular Structure
Using alpha-lytic protease, we have pursued the mechanisms of enzyme specificity and explored fundamental concepts of protein stability and folding. This understanding is now being applied to the problem of nuclear receptor signaling.
In parallel, we have made significant progress in analyzing the structure of centrosomes and chromosomes.
Throughout, we have pioneered the development of new methods for determining structure at both the cellular and molecular levels.
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Latest Publications
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Using fluorescence polarization anisotropy, we report the binding affinity of fluorescein-labeled compounds to Hsp90 in its open state and ATP-dependent closed state.
Leslie D. Alexander, James R. Partridge, David A. Agard, and Shelli R McAlpine |
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The crystal structure of human GCP4 shows that its C-terminal domain binds directly to γ-tubulin. The human GCP4 structure is the prototype for all GCPs, as it can be precisely positioned within the γTuSC envelope, revealing the nature of protein-protein interactions and conformational changes regulating nucleation activity.
Atsushi Matsuda, Lin Shao, Jerome Boulanger, Charles Kervrann, Peter M. Carlton, Peter Kner, David Agard, John W. Sedat
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