Research

I am interested in studying the crystal structures and structure function relationship of g-tubulin complex proteins. g-tubulin complex proteins in the centrosome play a key role in microtubule nucleation and most of them belong to a unique protein family. A full understanding of the function and mechanism would require the atomic resolution structures. The crystal structure of human g-tubulin has been solved in the lab, which provides critical information on the g-tubulin contact interface. Structures of the g-tubulin complex proteins and in complex with g-tubulin may offer more insight in regulation of the microtubule nucleation.

Publications

Huang K, Ferrin-O'Connell I, Zhang W, Leonard GA, O’Shea EK, Quiocho FA. “Structure of the Pho85-Pho80 CDK-Cyclin Complex of the Phosphate-Responsive Signal Transduction Pathway” Mol Cell. 2007 Nov 30;28(4):614-23.

Lee Y, Huang K, Quiocho D, O'Shea EK. “Molecular basis of cyclin-CDK-CKI regulation by reversible binding of an inositol pyrophosphate” Nature Chemical Biology 4, 25-32 (2007)