Chris Waddling

x-ray facility manager

Room S126C
Department of Biochemistry & Biophysics
University of California, San Francisco
Mission Bay, Genentech Hall
600 16th St.
San Francisco CA
94143-2140
(415) 476-8288 (Ph)
(415) 514-4142 (Fax)

As the X-ray facility manager, I fill a broad-ranging and dynamic position that includes maintenance of our two Raxis-IV machines, x-ray safety training, and coordination and maintenance of our facility's users and software.

Along with this, I am also involved in current research projects in the Agard lab. This includes work with Cynthia Fuhrmann on the refinement of a sub-angstrom structure of a mutant of alpha-lytic protease complexed with a BoroAla transition state mimic, as shown in the figure below:

I came to UCSF from Albany, NY, where I completed a two-year post doctorate at the Wadsworth Center with Dr. Patrick Van Roey. There, I was involved in the determination of several crystal structures. These included a high-resolution structure of the peptide-N-acetyl glycoamidase F mutant in complexation with its glycopeptide substrate, the endoglucosidase Endo F3, and complexes with its products, a deletion mutant of human thymadylate synthase complexed with dUMP and the antifolate drug Tomudex, and a novel intertwined structure of the DNA-binding domain of intron endonuclease I-TevI with its substrate.

I earned my Ph.D. in 1998 in Inorganic Chemistry, specializing in small molecule crystallography, at the University of Hawaii in Honolulu with Dr. Roger Cramer. There, I focused on the synthesis, kinetics, and structural determination of inclusion complexes of a cyclic pyrimidiniophane.

I earned my M.Sc. in 1993 in Inorganic Chemistry from Queen's University in Kingston, Ontario, Canada, with Dr. Donal Macartney, specializing in reaction mechanisms of inclusion complex formation using alpha-cyclodextrin.

I earned my B.Sc. in 1991 in Chemistry and Physics from the University of Toronto in Toronto, Ontario, where I studied main-group inorganic polymers with Dr. Ian Manners.

Publications (complete list of 165 articles and one patent citing the articles below)

"Crystal structures of the dinitrate-bis-(acetylenedicarboxylate) and acetylenedicarboxylate pseudorotaxane complexes of [24-pyrimidinium crown 6]," C.A. Waddling and R.E. Cramer, J. Incl. Phen. and Macro. Chem., 2008, 60(1-2), 1-8 (pdf or html, abstract online).

"Intertwined Structure of the DNA-Binding Domain of Intron Endonuclease I-TevI with its Substrate: Functional and Evolutionary Insights", P. VanRoey, C.A. Waddling, K.M. Fox, M. Belfort,  and V. Derbyshire, EMBO J., 2001, 20(14), 3631-7. (pdf or html, PDB Code 1I3J, articles citing this article)

"Crystal Structure of a Deletion Mutant of Human Thymidylate Synthase D(7-29) and its Ternary Complex with Tomudex and dUMP," R. Almog, C.A. Waddling, F. Maley, G. Maley, and P. VanRoey, Protein Science, 2001, 10(5), 988-996. (pdf or html, PDB Code 1HZW and 1I00, articles citing this article)

"Structural Basis for the Substrate Specificity of Endo-b-N-acetylglucosamine F₃," C.A. Waddling, T.H. Plummer, A.L. Tarentino, and P. VanRoey, Biochemistry, 2000, 39(27), 7878-85. (pdf or html, PDB Code 1EOK and 1EOM, articles citing this article)

"Structurally Novel Bioconversion Products of the Marine Natural Product Sarcophine Effectively Inhibit JB6 Cell Transformation," K.A. El Sayed, M.T. Hamann, C.A. Waddling, C. Jensen, S.K. Lee, C. Andersson Dunstan, and J.M. Pezzuto, J. Org. Chem., 1998, 63(21), 7449-55. (pdf or html, articles citing this article)

"Crystal Structures of the Nitrate Salt of 24-Pyrimidinium Crown 6 {5, 12, 19, 26, 33, 40 - hexamino - 3, 10, 17, 24, 31, 38 - hexamethyl [1·₆]-(1, 5)pyrimidinionphane and its Degradation Product in Water-Methanol Mixtures," R.E. Cramer, C.A. Waddling, C.H. Fujimoto, D.W. Smith, and K.E. Kim, J. Chem. Soc., Dalton Trans., 1997, 1675-83. (pdf, articles citing this article)

"Polymers with Sulfur(VI)-Nitrogen-Phosphorus Backbones:  Synthesis, Characterization, and Properties of Atactic Poly[(amino)thionylphosphazenes]," Y. Ni, P. Park, M. Liang, J. Massey, C. Waddling, and I. Manners, Macromolec., 1996, 29(10), 3401-8. (pdf or html, articles citing this article)

"Kinetic and Spectroscopic Studies of Symmetrical and Asymmetrical Pentascyano(N-heterocycle)ferrate(II) Rotaxanes of a-CD," D.H. Macartney and C.A. Waddling, Inorg. Chem., 1994, 33(25), 5912-19. (abstract or pdf and articles citing this article)

"Catalytic Elimination of Organics over Noble Metal Catalysts Deposited on Metal Structures of Low Mass," S. Suppiah, C. Waddling, and K.J. Kutchkoskie, Stud. Surf. Sci. Catal., 1992, 73 (Prog. Catal.), 187-95.

"Ring-opening polymerization as a route to new inorganic macromolecules, "M. Liang, C. Waddling, C. Honeyman, D. Foucher, I. Manners, Phosphorus, Sulfur and Silicon and the Related Elements 1992, 64(1-4), 113-20. (Patent citing this article)

"Poly(thionylphosphazenes); a new class of inorganic polymers with skeletal phosphorus, nitrogen, and sulfur(VI) atoms," M. Liang, C. Waddling, and I. Manners, Polymer Preprints, 1991, 32(3), 487-8. (articles citing this article)

Acknowledgements