Roger Cooke

Position:      Professor, Principal Investigator
Specialty:    EPR, Muscle Mechanics, Fluorescence
Phone:         (415) 476-4836
Fax:             (415) 476-1902
Email:          cooke@cgl.ucsf.edu

Research Interests:
     - Molecular Mechanism of Muscle Fatigue
    -  Structural and Biochemical Events that Allow Motor Proteins to Turn       Chemical Energy into Force and Motion

Selected Publications:

1.  Thomas, D.D. and Cooke, R.  Orientation of Spin-Labelled Myosin Heads in Glycerinated Muscle Fibers.  Biophys. J. 32:891-905 (1980).

2.  Cooke, R.  The Mechanism of Muscle Contraction.  Critical Reviews in Biochemistry, 21:53-118 (1986).

3.  Pate, E., Franks, K., Luciani, G. and Cooke, R.  The Inhibition of Muscle Contraction by Hydrogen Ions and Phosphate.  J. Physiol. 395:77-97 (1988).

4.  Pate, E., Bhimani, M., Franks-Skiba, K., and Cooke, R.  Reduced Effect Of pH On Skinned Rabbit Psoas Muscle Mechanics At High Temperatures: Implications For Fatigue.  J. Physiology 486:689-694 (1995).

5.  Pate, E., Naber, N., Matuska, M., Franks-Skiba, K., and Cooke, R.  Opening of the Myosin Nucleotide Triphosphate Binding Domain During the ATPase Cycle.  Biochemistry 36  (1997).

6. Rice, S., Lin, A.W., Safer, D., Hart, C.L., Naber, N., Carragher, B.O., Cain, S.M., Pechatnikova, E., Wilson-Kubalek, E.M., Whittaker, M., Pate, E., Cooke, R., Taylor, E.W., Milligan, R.A., and Vale, R.D.    A Structural Change in the Kinesin Motor Protein that Drives Motility.   Nature  402:778-784  (1999).

7. Rice, S., Cui, Y., Sindelar, C., Naber, N., Matuska, M., Vale, R., Cooke, R. Thermodynamic Properties of the Kinesin Neck Region Docking to the Catalytic Core Biophysical Journal, 84:1844-1854 (2003).

8.  Karatzaferi, C., Chinn, M.K., and Cooke, R.   The Force Exerted By A Muscle Crossbridge Depends Directly On The Strength Of the Actomyosin Bond. Biophysical J. 87:2532-2544 (2004)

9.  Naber, N. , Purcell, T., Pate, E., and Cooke, R.    Dynamics Of The Nucleotide Pocket Of Myosin Measured By Spin-Labeled Nucleotides.  Biophys. J. 92:172-184 (2007).

10.  Cooke R.    Modulation Of The Actomyosin Interaction During Fatigue Of Skeletal Muscle.   Muscle Nerve. 36(6):756-77 (2007).

11.   Karatzaferi, C. Franks-Skiba, K., Cooke, R.    The Inhibition Of Shortening Velocity Of Skinned Skeletal Muscle Fibers In Conditions That Mimic Fatigue.  Am J. Physiol Regul Integr Comp Physiol. 294:948-955 (2008).

12. Wong*, Y.L., Dietrich*, K.A., Naber, N., Cooke R., and Rice, S.E. The Kinesin-1 Tail Conformationally Restricts the Nucleotide Pocket. Biophysical J.  96:2799-2807 (2009)

13.  Stewart M., Franks-Skiba K., Chen S. and Cooke R. Myosin ATP Turnover Rate is a Mechanism Involved In Thermogenesis in Resting Skeletal Muscle Fibers. Proc Natl Acad Sci U S A. 107:430-5 (2010)

14. Purcell, T., Naber, N. Franks-Skiba, K., Dunn, R. A., Eldred, C. C., Berger, C. L. ,  Málnási-Csizmadia, A. Spudich, J. A. Swank, D. M.  Pate, E., and Cooke, R. Nucleotide Pocket Thermodynamics Measured by EPR Reveal How Energy Partitioning Relates Speed to Efficiency. J Mol Biol. 407(1):79-91 (2011)