Roger Cooke

Position:      Professor, Principal Investigator
Specialty:    EPR, Muscle Mechanics, Fluorescence
Phone:         (415) 476-4836
Fax:             (415) 476-1902
Email:          cooke@cgl.ucsf.edu

Research Interests:
     - Molecular Mechanism of Muscle Fatigue
    -  Structural and Biochemical Events that Allow Motor Proteins to Turn       Chemical Energy into Force and Motion

Selected Publications:

• Cooke, R.  The Actomyosin Interaction in Striated Muscle.  Physiological Rev.  77: 671-697 (1997).
  
  
• Pate, E., Franks-Skiba, K., Cooke, R.  Depletion Of Phosphate In Active Muscle Probes Actomyosin States Within The Powerstroke.  Biophysical J. 74:369-380 (1998).
  
  
• Gollub, J., Cremo, C.R. and Cooke, R. The ADP-Induced Rotation Of The Light Chain Domain Of Smooth Muscle Myosin Is Regulated By Myosin Phosphorylation.  Biochemistry 38:10107-10118 (1999).
  
  
• Rice, S., Lin, A.W., Safer, D., Hart, C.L., Naber, N., Carragher, B.O., Cain, S.M., Pechatnikova, E., Wilson-Kubalek, E.M., Whittaker, M., Pate, E., Cooke, R., Taylor, E.W., Milligan, R.A., and Vale, R.D.    A Structural Change in the Kinesin Motor Protein that Drives Motility.   Nature  402:778-784  (1999).
  
  
• Rice, S., Cui, Y., Sindelar, C., Naber, N., Matuska, M., Vale, R., Cooke, R. Thermodynamic Properties of the Kinesin Neck Region Docking to the Catalytic Core Biophysical Journal, 84:1844-1854 (2003).  
  
  
• Naber, N., Minehardt, T.J., Rice, S., Chen, X., Grammer, J., Matuska, M., Car, R.,  Vale, R.D., Kollman, P.A., Yount, R.G., Cooke, R., and Pate, E..  The nucleotide pocket of kinesin-family motors closes upon binding to microtubules Science 300:798-801 (2003).  
  
  
• Karatzaferi, C., Chinn, M.K., and Cooke, R.   The Force Exerted By A Muscle Crossbridge Depends Directly On The Strength Of the Actomyosin Bond. Biophysical J. 87:2532-2544 (2004) 
  
  
• Naber, N. , Purcell, T., Pate, E., and Cooke, R.    Dynamics Of The Nucleotide Pocket Of Myosin Measured By Spin-Labeled Nucleotides.  Biophys. J. 92:172-184 (2007). 
  
  
• Franks-Skiba, K., Lardelli, R., Goh, G., and Cooke, R.    Myosin Light Chain Phosphorylation Inhibits Muscle Affects Fiber Shortening Velocity In The Presence Of Vanadate.  Am. J. Physiology 292:R1603-12. (2007). 
  
  
• Cooke R.    Modulation Of The Actomyosin Interaction During Fatigue Of Skeletal Muscle.   Muscle Nerve. 36(6):756-77 (2007).  
  
  
• Karatzaferi, C. Franks-Skiba, K., Cooke, R.    The Inhibition Of Shortening Velocity Of Skinned Skeletal Muscle Fibers In Conditions That Mimic Fatigue.  Am J. Physiol Regul Integr Comp Physiol. 294:948-955 (2008).