Electron Tomography

Since the early 1980's we have been involved in the development of technologies iutilized in many aspects of electron tomography. Whether improving biological sample preparations, pioneering the use of CCD based imaging, automating data collection or developing software to optimize alignment and reconstruction we have been devoted to generating to the best possible data. Of course, all of this was driven by the desire to observe and understand complex three dimensional biological structures. Over the years we have had a number of versions of our software. The current version takes advantage of the microscopes stage geometry in order to predict image movement in x,y,and z during tilting. The result is a simple robust program that is can be used for standard, cryo, or double tilt tomography.

For details, download the following:

Zheng et al J Structural Biol 147 (2004) 91-101

UCSF Tomography Software

UCSF Tomography is used to collect single-axis tilt series on electron transimission microscopes. This software was implemented based upon a novel approach in which the compustage tilting is modeled as geometric rotation. The spatial movement of the sample as a result of stage tilting can be predicted based upon previously collected tomographic images. Therefore, there is no need to collect tracking and focusing images during the entire data collection. A significant dose saving can thus be achieved and is critical in collecting cryo tilt series. This software has been proven to be very robust in collecting cryo tilt series on thin and thick samples at various magnifications up to 60,000x.

This software is free for non-commercial use and provided "AS IS". By downloading UCSF Tomography, you agree to all the terms and conditions defined in the document "Terms & Conditions" accompanying this software.

Download UCSF Tomography

NOTE: This software may only be downloaded and used by academic and/or non-profit users. All others are required to contact David Agard for licensing information.

CCD (updated 2/8/2013)

K2 (updated 07/01/2016)