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Sedat Lab Publications List

Schermelleh L, Carlton PM, Haase S, Shao L, Winoto L, Kner P, Burke B, Cardoso MC, Agard DA, Gustafsson MG, Leonhardt H, Sedat JW. Subdiffraction multicolor imaging of the nuclear periphery with 3D structured illumination microscopy. Science. 2008 Jun 6;320(5881):1332-6.

Chang HH, Falick AM, Carlton PM, Sedat JW, Derisi JL, Marletta MA. N-terminal processing of proteins exported by malaria parasites. Mol Biochem Parasitol. 2008 May 2. [Epub ahead of print]

Pielage J, Cheng L, Fetter RD, Carlton PM, Sedat JW, Davis GW. A presynaptic giant ankyrin stabilizes the NMJ through regulation of presynaptic microtubules and transsynaptic cell adhesion. Neuron. 2008 Apr 24;58(2):195-209.

Gustafsson MG, Shao L, Carlton PM, Wang CJ, Golubovskaya IN, Cande WZ, Agard DA, Sedat JW. Three-dimensional resolution doubling in wide-field fluorescence microscopy by structured illumination. Biophys J. 2008 Jun;94(12):4957-70. Epub 2008 Mar 7.

Shao L, Isaac B, Uzawa S, Agard DA, Sedat JW, Gustafsson MG. I5S: wide-field light microscopy with 100-nm-scale resolution in three dimensions. Biophys J. 2008 Jun;94(12):4971-83. Epub 2008 Mar 7.

Hom EF, Marchis F, Lee TK, Haase S, Agard DA, Sedat JW. AIDA: an adaptive image deconvolution algorithm with application to multi-frame and three-dimensional data. J Opt Soc Am A Opt Image Sci Vis. 2007 Jun;24(6):1580-600.

Kam Z, Kner P, Agard D, Sedat JW. Modelling the application of adaptive optics to wide-field microscope live imaging. J Microsc. 2007 Apr;226(Pt 1):33-42.

Krug R, Carballido-Gamio J, Burghardt AJ, Haase S, Sedat JW, Moss WC, Majumdar S. Wavelet-based characterization of vertebral trabecular bone structure from magnetic resonance images at 3 T compared with micro-computed tomographic measurements. Magn Reson Imaging. 2007 Apr;25(3):392-8. Epub 2006 Nov 14.

König P, Braunfeld MB, Sedat JW, Agard DA. The three-dimensional structure of in vitro reconstituted Xenopus laevis chromosomes by EM tomography. Chromosoma. 2007 Aug;116(4):349-72. Epub 2007 Feb 28.

Zheng SQ, Kollman JM, Braunfeld MB, Sedat JW, Agard DA. Automated acquisition of electron microscopic random conical tilt sets. J Struct Biol. 2007 Jan;157(1):148-55. Epub 2006 Nov 3.

Zheng SQ, Keszthelyi B, Branlund E, Lyle JM, Braunfeld MB, Sedat JW, Agard DA. UCSF tomography: an integrated software suite for real-time electron microscopic tomographic data collection, alignment, and reconstruction. J Struct Biol. 2007 Jan;157(1):138-47. Epub 2006 Jun 23.

Vazquez J, Müller M, Pirrotta V, Sedat JW. The Mcp element mediates stable long-range chromosome-chromosome interactions in Drosophila. Mol Biol Cell. 2006 May;17(5):2158-65. Epub 2006 Feb 22.

Moss WC, Haase S, Lyle JM, Agard DA, Sedat JW. A novel 3D wavelet-based filter for visualizing features in noisy biological data. J Microsc. 2005 Aug;219(Pt 2):43-9.

Lowenstein MG, Goddard TD, Sedat JW. Long-range interphase chromosome organization in Drosophila: a study using color barcoded fluorescence in situ hybridization and structural clustering analysis. Mol Biol Cell. 2004 Dec;15(12):5678-92. Epub 2004 Sep 15.

Hanser, B.M., Gustafsson, M.G.L., Agard, D.A., and Sedat, J.W. (2004). Phase-Retrieved Pupil Functions in Wide-Field Fluorescence Microscopy. J. of Microscopy, in publication.

Zheng, Q.S., Braunfeld, M.B., Sedat, J.W., Agard, D.A. (2004). An improved strategy for automated electron microscopic tomography. J Structural Biology, 147, 91-101.

Hanser, B.M., Gustafsson, M.G.L., Agard, D.A., and Sedat, J.W. (2003). Phase retrieval for high-numerical-aperture optical systems. Optics Letters, 28, 801-803.

Vazquez, J., Belmont, A.S., and Sedat, J.W. (2002). The dynamics of homologous chromosome pairing during male Drosophila meiosis. Current Biology 12, 1473-1483.

Hanser, B., Gustafsson, M.G.L., Agard, D.A., and Sedat, J.W. (2002).Application of Phase Retrieved Pupil Functions in Wide-Field Microscopy. Proceedings of SPIE 4621, 40-46.

Hanser, B., Gustaffson, M.G.L., Agard, D.A., Sedat., J.W. (2001). Phase Retrieval of Widefield Microscopy Point Spread Functions. Three Dimensional and Multidimensional Microscopy: Image Acquisition and Processing VIII, Jose-Angel Conchello, Carol J. Cogswell, Tony Wilson, Editors, Proceedings of SPIE Vol. 4261, p. 60-68.

Vazquez, J., Belmont, A.S., and Sedat, J.W. (2001). Multiple regimes of constrained chromosome motion are regulated in the interphase Drosophila nucleus. Current Biology 11: 1227-1239.

Marshall, W.F., Marko, J.F., Agard, D.A., and Sedat, J.W. (2001). Chromosome elasticity and mitotic polar ejection force measured in living Drosophila embryos by four-dimesional microscopy-based motion analysis. Current Biology 11: 569-578.

Grogan, J.L., Mohrs, M., Harmon, B., Lacy, D.A., Sedat, J.W., and Locksley, R.M. (2001). Early transcription and silencing of cytokine genes underlie polarization of T helper cells subsets. Immunity 14: 205-215.

Kam, Z., Hanser, B., Gustafsson, M.G.L., Agard, D.A., and Sedat, J.W. (2001). Computational adaptive optics for live three-dimensional biological imaging. PNAS 98: 3790-3795.

Bass, H.W., Riera-Lizarazu, O., Ananiev, E.V., Bordoli, S.J., Rines, H.W., Phillips, R.L., Sedat, J.W., Agard, D.A., and Cande, W.Z. (2000). Evidence for the coincident initiation of homolog pairing and synapsis during the telomere-clustering (bouquet) stage of meiotic prophase. J. of Cell Science 113: 1033-1042.

Servant, G., Weiner, O.D., Herzmark, P., Balla, T., Sedat, J.W. and Bourne, H.R. (2000). Polarization of chemoattractant receptor signaling during neutrophil cheotaxis. Science 287: 1037-1040.

Fung, J.C., Sedat, J.W., and Marshall, W.F. (2000). Homologous DNA interactions in interphase: spatial organization of interphase nucleus. In Encylopedia of Life Sciences, Nature Publishing Group, London UK.

Gustafsson, M.G., Agard, D.A. and Sedat, J.W. (1999). I5M: 3D widefield light microscopy with better than 100 nm axial resolution. J. Microscopy 195: 10-16.

Weiner, O.D., Servant, G., Welch, M.D., Mitchison, T.J., Sedat, J.W., and Bourne, H.R. (1999). Spatial control of actin polymerization during neutrophil chemotaxis. Nature Cell Biol. 1: 75-81.

Servant, G., Weiner, O.D., Neptune, E.R., Sedat, J.W. and Bourne, H.R. (1999). Dynamics of a chemoattractant receptor in living neutrophils during chemotaxis. Molec. Biol. Cell 10: 1163-1178.

Marshal, W.F., and Sedat, J.W. (1999). Nuclear Architecture. In Genomic Imprinting: an interdisciplinary approach. Rolf Ohlsson , ed., Springer-Verlag. pp 283-301.

Kalman, D., Weiner, O.D., Goosney, D.L., Sedat, J.W., Finlay, B.B., Abo, A., and Bishop, J.M. (1999). Enteropathogenic E. coli acts through WASP and Arp 2/3 complex to form actin pedestals. Nature Cell Biol. 1: 389-391.

Straight, A.F., Sedat, J.W. and Murray, A.W. (1998). Time-lapse microscopy reveals unique roles for kinesins during anaphase in budding yeast. J. Cell. Biol. 143:687-694.

Dernburg, A.F. and Sedat, J.W. (1998). Mapping three-dimensional chromosome architecture in situ. Methods in Cell Biology 53:187-233.

Laurer, J.H., Hajduk, D.A., Fung, J.C., Sedat, J.W., Smith, S.D., Gruner, S.M., Agard, D.A. and Spontak, R.J. (1998). Microstructural analysis of a cubic bicontinuous morphology in a neat SIS triblock copolymer. Macromolecules 30:3938-3941.

Fung, J.C., Marshall, W.F., Dernburg, A., Agard, D.A. and Sedat, J.W. (1998). Homologous chromosome pairing in Drosophila melanogaster proceeds through multiple initiations. J. Cell. Biol. 141:5-20.

Hiraoka, Y., Agard, D. and Sedat, J. (1997). Spatial arrangement of homologous chromosomes during anaphase in early embryos of Drosophila melanogaster studied by three-dimensional fluorescence microscopy. Bioimaging 5:183-193.

Marshall WF; Straight A; Marko JF; Swedlow J; Dernburg A; Belmont A; Murray AW; Agard DA; Sedat JW. 1997. Interphase chromosomes undergo constrained diffusional motion in living cells. Current Biology 7,930-939.

Straight AF; Marshall WF; Sedat JW; Murray AW. 1997. Mitosis in living budding yeast: anaphase A but no metaphase plate. Science 277,574-578.

Oegema, K; Marshall WF; Sedat JW; Alberts BM. 1997. Two proteins that cycle asynchronously between centrosomes and nuclear structures: Drosophila CP60 and CP190. J. Cell Sci. 110,1573-1583.

Nunnari J; Marshall WF; Straight A; Murray A; Sedat JW; Walter P. 1997. Mitochondrial transmission during mating in Saccharomyces cerevisiae is determined by mitochondrial fusion and fission and the intramitochondrial segregation of mitochondrial DNA. Mol. Biol. of the Cell 8,1233-1242.

Marshall WF; Fung JC; Sedat JW. 1997. Deconstructing the nucleus: global architecture from local interactions. Curr. Opin. Gen. Dev. 7,259-263.

Bass HW; Marshall WF; Sedat JW; Agard DA; Cande WZ. 1997. Telomeres cluster de novo before the initiation of synapsis; a 3-dimensional spatial analysis of telomere positions before and during meiotic prophase. J. Cell Biol. 137,5-18.

Kirk KE; Harmon BP; Reichardt IK; Sedat JW; Blackburn EH. 1997. Block in anaphase chromosome separation caused by a telomerase template mutation. Science 275,1478-81.

Han KR; Gubbens AJ; Sedat JW; Agard DA. 1996. Optimal strategies for imaging thick biological specimens - exit wavefront reconstruction and energy-filtered imaging. J. Microscopy 183,124-132.

Dernburg AF; Daily DR; Yook KJ; Corbin JA; Sedat JW; Sullivan W. 1996. Selective loss of sperm bearing a compound chromosome in the Drosophila female. Genetics 143,1629-1642.

Dernburg AF; Sedat JW; Hawley RS. 1996. Direct evidence of a role for heterochromatin in meiotic chromosome segregation. Cell 86,135-146.

Spontak RJ; Fung JC; Braunfeld MB; Sedat JW; et al. 1996. Phase behavior of ordered diblock copolymer blends - effect of compositional heterogeneity. Macromolecules 29,4494-4507.

Dernburg AF; Broman KW; Fung JC; Marshall WF; Agard DA; Sedat JW. 1996. Perturbation of nuclear architecture by long-distance chromosome interactions. Cell 85,745-759.

Marshall WF; Dernburg AF; Harmon B; Agard DA; Sedat JW. 1996. Specific interactions of chromatin with the nuclear envelope: Positional determination within the nucleus in Drosophila melanogaster. Molecular Biology of the Cell 7,825-842.

Scalettar BA; Swedlow JR; Sedat JW; Agard DA. 1996. Dispersion, aberration and deconvolution in multi-wavelength fluorescence images. Journal of Microscopy 182,50-60.

Spontak RJ; Fung JC; Braunfeld MB; Sedat JW; et al. 1996. Architecture-induced phase immiscibility in a diblock/multiblock copolymer blend. Macromolecules 29,2850-2856.

Paddy MR; Saumweber H; Agard DA; Sedat JW. 1996. Time-resolved, in vivo studies of mitotic spindle formation and nuclear lamina breakdown in Drosophila early embryos. Journal of Cell Science 109,591-607.

Chen H; Hughes DD; Chan TA; Sedat JW; Agard DA. 1996. IVE (image visualization environment) - A software platform for all three-dimensional microscopy applications. Journal of Structural Biology 116,56-60.

Fung JC; Liu WP; de Ruijter W; Chen H; Abbey CK; Sedat JW; Agard DA. 1996. Toward fully automated high-resolution electron tomography. Journal of Structural Biology 116,181-189

Moritz M; Braunfeld MB; Sedat JW; Alberts B; Agard DA. 1995. Microtubule nucleation by gamma-tubulin-containing rings in the centrosome. Nature 378(6557):638-40.

Urata Y; Parmelee SJ; Agard DA; Sedat JW. 1995. A three-dimensional structural dissection of Drosophila polytene chromosomes. Journal of Cell Biology 131(2):279-295.

Moritz M; Braunfeld MB; Fung JC; Sedat JW; Alberts BM; Agard DA. 1995. Three-dimensional structural characterization of centrosomes from early Drosophila embryos. Journal of Cell Biology 130(5):1149-59.

Han KF; Sedat JW; Agard DA. 1995. Mechanism of image formation for thick biological specimens: exit wavefront reconstruction and electron energy-loss spectroscopic imaging. Journal of Microscopy 178 ( Pt 2):107-19.

Braunfeld MB; Koster AJ; Sedat JW; Agard DA. 1994. Cryo automated electron tomography: towards high-resolution reconstructions of plastic-embedded structures. Journal of Microscopy 174 ( Pt 2):75-84.

Dawe RK; Sedat JW; Agard DA; Cande WZ. 1994. Meiotic chromosome pairing in maize is associated with a novel chromatin organization. Cell 76(5):901-12.

Swedlow JR; Sedat JW; Agard DA. 1993. Multiple chromosomal populations of topoisomerase II detected in vivo by time-lapse, three-dimensional wide-field microscopy. Cell 73(1):97-108.

Hiraoka Y; Dernburg AF; Parmelee SJ; Rykowski MC; Agard DA; Sedat JW. 1993. The onset of homologous chromosome pairing during Drosophila melanogaster embryogenesis. Journal of Cell Biology 120(3):591-600.

Hiraoka Y; Agard DA; Sedat JW. 1990. Temporal and spatial coordination of chromosome movement, spindle formation, and nuclear envelope breakdown during prometaphase in Drosophila melanogaster embryos. Journal of Cell Biology 111(6 Pt 2):2815-28.

Paddy MR; Belmont AS; Saumweber H; Agard DA; Sedat JW. 1990. Interphase nuclear envelope lamins form a discontinuous network that interacts with only a fraction of the chromatin in the nuclear periphery. Cell 62(1):89-106.

Hiraoka Y; Minden JS; Swedlow JR; Sedat JW; Agard DA. 1989. Focal points for chromosome condensation and decondensation revealed by three-dimensional in vivo time-lapse microscopy. Nature 342(6247):293-6.

Belmont AS; Braunfeld MB; Sedat JW; Agard DA. 1989. Large-scale chromatin structural domains within mitotic and interphase chromosomes in vivo and in vitro. Chromosoma 98(2):129-43.

Minden JS; Agard DA; Sedat JW; Alberts BM. 1989. Direct cell lineage analysis in Drosophila melanogaster by time-lapse, three-dimensional optical microscopy of living embryos. Journal of Cell Biology 109(2):505-16.

Mathog D; Sedat JW. 1989. The three-dimensional organization of polytene nuclei in male Drosophila melanogaster with compound XY or ring X chromosomes. Genetics 121(2):293-311.