Stroud Lab / MPEC / CSMP
CONSTRUCT Log
YOUR INFORMATION
First Name
*
:
Last Name
*
:
Email Address
*
:
Submit to :
4th floor
1st floor
PROTEIN
Protein Name (Ex. amtB)
*
:
Cistron Number
*
:
1
2
3
4
of
1
2
3
4
Species
*
:
H.sapiens
D.melanogaster
M.musculus
S.cerevisiae
C.elegans
R.norvegicus
E.coli
A.thaliana
P.furiosus
T.maritima
P.pastoris
D.radiodurans
Other
Codon Optimized for Species:
E.coli
S.cerevisiae
P.pastoris
SF9
Other
Genebank ID (Ex.GI:1103927 from NCBI Protein DB):
SwissProt ID (Ex. P69681):
Mutations (Ex. Y96W):
Status:
Expressed
Purified
Crystalized
Cloning
Genebank File (Vector and Insert) :
(
*
required for first cistron only)
Vector Common Name (Ex. pET29b)
*
:
Archive ID for Vector (Ex. FGA0002)
*
:
Cloning Method
*
:
LIC
Gateway
Ligation
3' Restriction Enzyme
5' Restriction Enzyme
Other
Tags for N-term
*
:
6His
10His
MBP
GFP
S-Tag
T7
8His
Flag
RFP
Mistic
Cys
None
Other
Protease Cleavage for N-term
*
:
Thrombin
TEV
3C
None
Other
Tags for C-term
*
:
6His
10His
MBP
GFP
S-Tag
T7
8His
Flag
RFP
Mistic
Cys
None
Other
Protease Cleavage for C-term
*
:
Thrombin
TEV
3C
None
Other
ADDITIONAL COMMENTS OR CHANGES
NOTEBOOK INFORMATION
Notebook Name
*
:
Page Number(s)
*
:
*
Required Field