John Gross, PhD

Enhanced NF-κB activation via HIV-1 Tat-TRAF6 cross-talk.

Science advances

Li Y, Liu X, Fujinaga K, Gross JD, Frankel AD

The structural basis for HIV-1 Vif antagonism of human APOBEC3G.

Nature

Li YL, Langley CA, Azumaya CM, Echeverria I, Chesarino NM, Emerman M, Cheng Y, Gross JD

A call to order: Examining structured domains in biomolecular condensates.

Journal of magnetic resonance (San Diego, Calif. : 1997)

Tibble RW, Gross JD

SIRT5 is a proviral factor that interacts with SARS-CoV-2 Nsp14 protein.

PLoS pathogens

Walter M, Chen IP, Vallejo-Gracia A, Kim IJ, Bielska O, Lam VL, Hayashi JM, Cruz A, Shah S, Soveg FW, Gross JD, Krogan NJ, Jerome KR, Schilling B, Ott M, Verdin E

Fluorescence-Based Activity Screening Assay Reveals Small Molecule Inhibitors of Vaccinia Virus mRNA Decapping Enzyme D9.

ACS chemical biology

Bednarczyk M, Peters JK, Kasprzyk R, Starek J, Warminski M, Spiewla T, Mugridge JS, Gross JD, Jemielity J, Kowalska J

Structure of the poxvirus decapping enzyme D9 reveals its mechanism of cap recognition and catalysis.

Structure (London, England : 1993)

Peters JK, Tibble RW, Warminski M, Jemielity J, Gross JD

SIRT5 is a proviral factor that interacts with SARS-CoV-2 Nsp14 protein.

bioRxiv : the preprint server for biology

Walter M, Chen IP, Vallejo-Gracia A, Kim IJ, Bielska O, Lam VL, Hayashi JM, Cruz A, Shah S, Gross JD, Krogan NJ, Schilling B, Ott M, Verdin E

Identification of recombinant Fabs for structural and functional characterization of HIV-host factor complexes.

PloS one

Sevillano N, Green EM, Votteler J, Kim DY, Ren X, Yang B, Liu X, Lourenço AL, Hurley JH, Farr-Jones S, Gross JD, Cheng Y, Craik CS

Biomolecular condensates amplify mRNA decapping by biasing enzyme conformation.

Nature chemical biology

Structural Insights into the Interaction of Clinically Relevant Phosphorothioate mRNA Cap Analogs with Translation Initiation Factor 4E Reveal Stabilization via Electrostatic Thio-Effect.

ACS chemical biology

Warminski M, Kowalska J, Nowak E, Kubacka D, Tibble R, Kasprzyk R, Sikorski PJ, Gross JD, Nowotny M, Jemielity J

Pat1 increases the range of decay factors and RNA bound by the Lsm1-7 complex.

RNA (New York, N.Y.)

Lobel JH, Gross JD

Biophysical Properties of HP1-Mediated Heterochromatin.

Cold Spring Harbor symposia on quantitative biology

Sanulli S, Gross JD, Narlikar GJ

A SARS-CoV-2 protein interaction map reveals targets for drug repurposing.

Nature

Gordon DE, Jang GM, Bouhaddou M, Xu J, Obernier K, White KM, O'Meara MJ, Rezelj VV, Guo JZ, Swaney DL, Tummino TA, Huettenhain R, Kaake RM, Richards AL, Tutuncuoglu B, Foussard H, Batra J, Haas K, Modak M, Kim M, Haas P, Polacco BJ, Braberg H, Fabius JM, Eckhardt M, Soucheray M, Bennett MJ, Cakir M, McGregor MJ, Li Q, Meyer B, Roesch F, Vallet T, Mac Kain A, Miorin L, Moreno E, Naing ZZC, Zhou Y, Peng S, Shi Y, Zhang Z, Shen W, Kirby IT, Melnyk JE, Chorba JS, Lou K, Dai SA, Barrio-Hernandez I, Memon D, Hernandez-Armenta C, Lyu J, Mathy CJP, Perica T, Pilla KB, Ganesan SJ, Saltzberg DJ, Rakesh R, Liu X, Rosenthal SB, Calviello L, Venkataramanan S, Liboy-Lugo J, Lin Y, Huang XP, Liu Y, Wankowicz SA, Bohn M, Safari M, Ugur FS, Koh C, Savar NS, Tran QD, Shengjuler D, Fletcher SJ, O'Neal MC, Cai Y, Chang JCJ, Broadhurst DJ, Klippsten S, Sharp PP, Wenzell NA, Kuzuoglu D, Wang HY, Trenker R, Young JM, Cavero DA, Hiatt J, Roth TL, Rathore U, Subramanian A, Noack J, Hubert M, Stroud RM, Frankel AD, Rosenberg OS, Verba KA, Agard DA, Ott M, Emerman M, Jura N, von Zastrow M, Verdin E, Ashworth A, Schwartz O, d'Enfert C, Mukherjee S, Jacobson M, Malik HS, Fujimori DG, Ideker T, Craik CS, Floor SN, Fraser JS, Gross JD, Sali A, Roth BL, Ruggero D, Taunton J, Kortemme T, Beltrao P, Vignuzzi M, García-Sastre A, Shokat KM, Shoichet BK, Krogan NJ

Structural Basis for a Species-Specific Determinant of an SIV Vif Protein toward Hominid APOBEC3G Antagonism.

Cell host & microbe

Binning JM, Chesarino NM, Emerman M, Gross JD

Pat1 activates late steps in mRNA decay by multiple mechanisms.

Proceedings of the National Academy of Sciences of the United States of America

Lobel JH, Tibble RW, Gross JD

HP1 reshapes nucleosome core to promote phase separation of heterochromatin.

Nature

Sanulli S, Trnka MJ, Dharmarajan V, Tibble RW, Pascal BD, Burlingame AL, Griffin PR, Gross JD, Narlikar GJ

ARIH2 Is a Vif-Dependent Regulator of CUL5-Mediated APOBEC3G Degradation in HIV Infection.

Cell host & microbe

Hüttenhain R, Xu J, Burton LA, Gordon DE, Hultquist JF, Johnson JR, Satkamp L, Hiatt J, Rhee DY, Baek K, Crosby DC, Frankel AD, Marson A, Harper JW, Alpi AF, Schulman BA, Gross JD, Krogan NJ

Conformational Dynamics of the HIV-Vif Protein Complex.

Biophysical journal

Ball KA, Chan LM, Stanley DJ, Tierney E, Thapa S, Ta HM, Burton L, Binning JM, Jacobson MP, Gross JD

Structural and molecular mechanisms for the control of eukaryotic 5'-3' mRNA decay.

Nature structural & molecular biology

Mugridge JS, Coller J, Gross JD

Decapping enzymes STOP "cancer" ribosomes in their tracks.

The EMBO journal

Mugridge JS, Gross JD

A Viral Protein Restricts Drosophila RNAi Immunity by Regulating Argonaute Activity and Stability.

Cell host & microbe

Nayak A, Kim DY, Trnka MJ, Kerr CH, Lidsky PV, Stanley DJ, Rivera BM, Li KH, Burlingame AL, Jan E, Frydman J, Gross JD, Andino R

CRL4AMBRA1 targets Elongin C for ubiquitination and degradation to modulate CRL5 signaling.

The EMBO journal

Chen SH, Jang GM, HÃ¼ttenhain R, Gordon DE, Du D, Newton BW, Johnson JR, Hiatt J, Hultquist JF, Johnson TL, Liu YL, Burton LA, Ye J, Reichermeier KM, Stroud RM, Marson A, Debnath J, Gross JD, Krogan NJ

Control of mRNA decapping by autoinhibition.

Nucleic acids research

Paquette DR, Tibble RW, Daifuku TS, Gross JD

Structure of the activated Edc1-Dcp1-Dcp2-Edc3 mRNA decapping complex with substrate analog poised for catalysis.

Nature communications

Mugridge JS, Tibble RW, Ziemniak M, Jemielity J, Gross JD

Fab-based inhibitors reveal ubiquitin independent functions for HIV Vif neutralization of APOBEC3 restriction factors.

PLoS pathogens

Binning JM, Smith AM, Hultquist JF, Craik CS, Caretta Cartozo N, Campbell MG, Burton L, La Greca F, McGregor MJ, Ta HM, Bartholomeeusen K, Peterlin BM, Krogan NJ, Sevillano N, Cheng Y, Gross JD

Application of a Schizosaccharomyces pombe Edc1-fused Dcp1-Dcp2 decapping enzyme for transcription start site mapping

RNA

DR Paquette, JS Mugridge, DE Weinberg, and JD Gross

Making Sense of Multifunctional Proteins: Human Immunodeficiency Virus Type 1 Accessory and Regulatory Proteins and Connections to Transcription.

Annual review of virology

Faust TB, Binning JM, Gross JD, Frankel AD

Biochemical Basis for Distinct Roles of the Heterochromatin Proteins Swi6 and Chp2.

Journal of molecular biology

Isaac RS, Sanulli S, Tibble R, Hornsby M, Ravalin M, Craik CS, Gross JD, Narlikar GJ

Distortion of histone octamer core promotes nucleosome mobilization by a chromatin remodeler.

Science (New York, N.Y.)

Sinha KK, Gross JD, Narlikar GJ

CBFß and HIV Infection.

Advances in experimental medicine and biology

Kim DY, Gross JD

Molecular biology: Messenger RNAs marked for longer life.

Nature

Weinberg DE, Gross JD

Structural basis of mRNA-cap recognition by Dcp1-Dcp2.

Nature structural & molecular biology

Mugridge JS, Ziemniak M, Jemielity J, Gross JD

Two-headed tetraphosphate cap analogs are inhibitors of the Dcp1/2 RNA decapping complex.

RNA (New York, N.Y.)

Ziemniak M, Mugridge JS, Kowalska J, Rhoads RE, Gross JD, Jemielity J

Thermodynamics of Rev-RNA interactions in HIV-1 Rev-RRE assembly.

Biochemistry

Jayaraman B, Mavor D, Gross JD, Frankel AD

Lineage-Specific Viral Hijacking of Non-canonical E3 Ubiquitin Ligase Cofactors in the Evolution of Vif Anti-APOBEC3 Activity.

Cell reports

Kane JR, Stanley DJ, Hultquist JF, Johnson JR, Mietrach N, Binning JM, Jónsson SR, Barelier S, Newton BW, Johnson TL, Franks-Skiba KE, Li M, Brown WL, Gunnarsson HI, Adalbjornsdóttir A, Fraser JS, Harris RS, Andrésdóttir V, Gross JD, Krogan NJ

A mutually assured destruction mechanism attenuates light signaling in Arabidopsis.

Science (New York, N.Y.)

Ni W, Xu SL, Tepperman JM, Stanley DJ, Maltby DA, Gross JD, Burlingame AL, Wang ZY, Quail PH

Active site conformational dynamics are coupled to catalysis in the mRNA decapping enzyme Dcp2.

Structure (London, England : 1993)

Aglietti RA, Floor SN, McClendon CL, Jacobson MP, Gross JD

The glucocorticoid receptor dimer interface allosterically transmits sequence-specific DNA signals.

Nature structural & molecular biology

Watson LC, Kuchenbecker KM, Schiller BJ, Gross JD, Pufall MA, Yamamoto KR

Judge, jury, and executioner: DXO functions as a decapping enzyme and exoribonuclease in pre-mRNA quality control.

Molecular cell

Mugridge JS, Gross JD

From systems to structure: bridging networks and mechanism.

Molecular cell

Fraser JS, Gross JD, Krogan NJ

CBFß stabilizes HIV Vif to counteract APOBEC3 at the expense of RUNX1 target gene expression.

Molecular cell

Kim DY, Kwon E, Hartley PD, Crosby DC, Mann S, Krogan NJ, Gross JD

Inhibition of a NEDD8 Cascade Restores Restriction of HIV by APOBEC3G.

PLoS pathogens

Stanley DJ, Bartholomeeusen K, Crosby DC, Kim DY, Kwon E, Yen L, Cartozo NC, Li M, Jäger S, Mason-Herr J, Hayashi F, Yokoyama S, Krogan NJ, Harris RS, Peterlin BM, Gross JD

In support of the BMRB.

Nature structural & molecular biology

Markley JL, Akutsu H, Asakura T, Baldus M, Boelens R, Bonvin A, Kaptein R, Bax A, Bezsonova I, Gryk MR, Hoch JC, Korzhnev DM, Maciejewski MW, Case D, Chazin WJ, Cross TA, Dames S, Kessler H, Lange O, Madl T, Reif B, Sattler M, Eliezer D, Fersht A, Forman-Kay J, Kay LE, Fraser J, Gross J, Kortemme T, Sali A, Fujiwara T, Gardner K, Luo X, Rizo-Rey J, Rosen M, Gil RR, Ho C, Rule G, Gronenborn AM, Ishima R, Klein-Seetharaman J, Tang P, van der Wel P, Xu Y, Grzesiek S, Hiller S, Seelig J, Laue ED, Mott H, Nietlispach D, Barsukov I, Lian LY, Middleton D, Blumenschein T, Moore G, Campbell I, Schnell J, Vakonakis IJ, Watts A, Conte MR, Mason J, Pfuhl M, Sanderson MR, Craven J, Williamson M, Dominguez C, Roberts G, Günther U, Overduin M, Werner J, Williamson P, Blindauer C, Crump M, Driscoll P, Frenkiel T, Golovanov A, Matthews S, Parkinson J, Uhrin D, Williams M, Neuhaus D, Oschkinat H, Ramos A, Shaw DE, Steinbeck C, Vendruscolo M, Vuister GW, Walters KJ, Weinstein H, Wüthrich K, Yokoyama S

The crystal structure of the periplasmic domain of Vibrio parahaemolyticus CpxA.

Protein science : a publication of the Protein Society

Kwon E, Kim DY, Ngo TD, Gross CA, Gross JD, Kim KK

Interdomain dynamics and coactivation of the mRNA decapping enzyme Dcp2 are mediated by a gatekeeper tryptophan.

Proceedings of the National Academy of Sciences of the United States of America

Floor SN, Borja MS, Gross JD

Vif hijacks CBF-ß to degrade APOBEC3G and promote HIV-1 infection.

Nature

Jäger S, Kim DY, Hultquist JF, Shindo K, LaRue RS, Kwon E, Li M, Anderson BD, Yen L, Stanley D, Mahon C, Kane J, Franks-Skiba K, Cimermancic P, Burlingame A, Sali A, Craik CS, Harris RS, Gross JD, Krogan NJ