
Professor
Department of Biochemistry and Biophysics
+1 415 476-5143
Discovering the Structural Basis for Biological Function at the Molecular and Cellular Levels
Our research is focused on elucidating the mechanism of Hsp90 chaperone function and its role in human disease, microtubule nucleation and centrosome structure, and the structure and cell biology of phage-encoded tubulins.
In efforts to understand the spatial and temporal regulation of tubulin polymerization into microtubules (MTs), we seek to understand, in atomic detail, the molecular mechanisms underlying dynamic MT behavior and MT nucleation.
Accompanying this is an effort to develop new technologies for high-resolution cryoEM and fluorescence light microscopy.
Publications
Real-space heterogeneous reconstruction, refinement, and disentanglement of CryoEM conformational states with HetSIREN.
Nature communications
Deconvolution to restore cryo-EM maps with anisotropic resolution.
bioRxiv : the preprint server for biology
The structure of basal body inner junctions from Tetrahymena revealed by electron cryo-tomography.
The EMBO journal
A novel lncRNA FAM151B-DT regulates autophagy and degradation of aggregation prone proteins.
medRxiv : the preprint server for health sciences
Real-space heterogeneous reconstruction, refinement, and disentanglement of CryoEM conformational states with HetSIREN.
bioRxiv : the preprint server for biology
The Structure of Cilium Inner Junctions Revealed by Electron Cryo-tomography.
bioRxiv : the preprint server for biology
Characterization of a lipid-based jumbo phage compartment as a hub for early phage infection.
Cell host & microbe
Poc1 bridges basal body inner junctions to promote triplet microtubule integrity and connections.
The Journal of cell biology
Functionalized graphene-oxide grids enable high-resolution cryo-EM structures of the SNF2h-nucleosome complex without crosslinking.
Nature communications
Poc1 is a basal body inner junction protein that promotes triplet microtubule integrity and interconnections.
bioRxiv : the preprint server for biology
Cryo-EM reveals how Hsp90 and FKBP immunophilins co-regulate the glucocorticoid receptor.
Nature structural & molecular biology
The next decade in structural biology.
Structure (London, England : 1993)
Next-generation proteomics for quantitative Jumbophage-bacteria interaction mapping.
Nature communications
Functionalized graphene-oxide grids enable high-resolution cryo-EM structures of the SNF2h-nucleosome complex without crosslinking.
bioRxiv : the preprint server for biology
Hsp90 provides a platform for kinase dephosphorylation by PP5.
Nature communications
Next-generation interaction proteomics for quantitative Jumbophage-bacteria interaction mapping.
bioRxiv : the preprint server for biology
The ΦPA3 phage nucleus is enclosed by a self-assembling 2D crystalline lattice.
Nature communications
Cryo-EM reveals how Hsp90 and FKBP immunophilins co-regulate the Glucocorticoid Receptor.
bioRxiv : the preprint server for biology
Improved Analysis of Cross-Linking Mass Spectrometry Data with Kojak 2.0, Advanced by Integration into the Trans-Proteomic Pipeline.
Journal of proteome research
In situ cryo-electron tomography reveals the asymmetric architecture of mammalian sperm axonemes.
Nature structural & molecular biology
Solution of the protein structure prediction problem at last: crucial innovations and next frontiers.
Faculty reviews
Interactions between mTORC2 core subunits Rictor and mSin1 dictate selective and context-dependent phosphorylation of substrate kinases SGK1 and Akt.
The Journal of biological chemistry
AreTomo: An integrated software package for automated marker-free, motion-corrected cryo-electron tomographic alignment and reconstruction.
Journal of structural biology: X
Author Correction: Structures of the HER2-HER3-NRG1β complex reveal a dynamic dimer interface.
Nature
Electron cryo-tomography structure of axonemal doublet microtubule from Tetrahymena thermophila.
Life science alliance
Entropy-regularized deconvolution of cellular cryotransmission electron tomograms.
Proceedings of the National Academy of Sciences of the United States of America
Proteomic analysis of microtubule inner proteins (MIPs) in Rib72 null Tetrahymena cells reveals functional MIPs.
Molecular biology of the cell
CryoEM and AI reveal a structure of SARS-CoV-2 Nsp2, a multifunctional protein involved in key host processes.
Research square
CryoEM and AI reveal a structure of SARS-CoV-2 Nsp2, a multifunctional protein involved in key host processes.
bioRxiv : the preprint server for biology
Practical considerations for using K3 cameras in CDS mode for high-resolution and high-throughput single particle cryo-EM.
Journal of structural biology
Microtubules form by progressively faster tubulin accretion, not by nucleation-elongation.
The Journal of cell biology
Hsp70 chaperone blocks a-synuclein oligomer formation via a novel engagement mechanism.
The Journal of biological chemistry
[The basics of immobilisation].
Revue de l'infirmiere
Comparative host-coronavirus protein interaction networks reveal pan-viral disease mechanisms.
Science (New York, N.Y.)
General and robust covalently linked graphene oxide affinity grids for high-resolution cryo-EM.
Proceedings of the National Academy of Sciences of the United States of America
A molecular pore spans the double membrane of the coronavirus replication organelle.
Science (New York, N.Y.)
XMAP215 and ?-tubulin additively promote microtubule nucleation in purified solutions.
Molecular biology of the cell
Author Correction: Sas-4 provides a scaffold for cytoplasmic complexes and tethers them in a centrosome.
Nature communications
A SARS-CoV-2-Human Protein-Protein Interaction Map Reveals Drug Targets and Potential Drug-Repurposing.
bioRxiv : the preprint server for biology
Electron cryotomography of intact motile cilia defines the basal body to axoneme transition.
The Journal of cell biology
Amino and PEG-Amino Graphene Oxide Grids Enrich and Protect Samples for High-resolution Single Particle Cryo-electron Microscopy.
Journal of structural biology
Novel Small Molecules Targeting the Intrinsically Disordered Structural Ensemble of a-Synuclein Protect Against Diverse a-Synuclein Mediated Dysfunctions.
Scientific reports
Mind the gap: micro-expansion joints drastically decrease the bending of FIB-milled cryo-lamellae.
Journal of structural biology
Electron cryo-tomography provides insight into procentriole architecture and assembly mechanism.
eLife
Consideration of sample motion in cryo-tomography based on alignment residual interpolation.
Journal of structural biology
Competing protein-protein interactions regulate binding of Hsp27 to its client protein tau.
Nature communications
Insights into centriole geometry revealed by cryoTomography of doublet and triplet centrioles.
eLife
A simple and robust procedure for preparing graphene-oxide cryo-EM grids.
Journal of structural biology
Calcium binding to a remote site can replace magnesium as cofactor for mitochondrial Hsp90 (TRAP1) ATPase activity.
The Journal of biological chemistry
Cryo-tomography tilt-series alignment with consideration of the beam-induced sample motion.
Journal of structural biology
The Structural Asymmetry of Mitochondrial Hsp90 (Trap1) Determines Fine Tuning of Functional Dynamics.
Journal of chemical theory and computation
The centrosomin CM2 domain is a multi-functional binding domain with distinct cell cycle roles.
PloS one
Protein Expression and Purification of the Hsp90-Cdc37-Cdk4 Kinase Complex from Saccharomyces cerevisiae.
Bio-protocol
How Hsp90 and Cdc37 Lubricate Kinase Molecular Switches.
Trends in biochemical sciences
Symmetry broken and rebroken during the ATP hydrolysis cycle of the mitochondrial Hsp90 TRAP1.
eLife
NMR characterization of HtpG, the E. coli Hsp90, using sparse labeling with 13C-methyl alanine.
Journal of biomolecular NMR
Grafting both acute wound site and adjacent donor site with the same graft: an easy and safe procedure to improve healing and minimize pain in elderly and bedridden patients.
Annals of burns and fire disasters
Design of Allosteric Stimulators of the Hsp90 ATPase as New Anticancer Leads.
Chemistry (Weinheim an der Bergstrasse, Germany)
MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy.
Nature methods
Assembly of a nucleus-like structure during viral replication in bacteria.
Science (New York, N.Y.)
Internal Structure and Preferential Protein Binding of Colloidal Aggregates.
ACS chemical biology
Atomic structure of Hsp90-Cdc37-Cdk4 reveals that Hsp90 traps and stabilizes an unfolded kinase.
Science (New York, N.Y.)
Higher-order oligomerization of Spc110p drives ?-tubulin ring complex assembly.
Molecular biology of the cell
A Fortunate Story of an Unusual AK-47 Bullet Trajectory: Always Keep a Smartphone in Your Pocket.
Prehospital and disaster medicine
The Seckel syndrome and centrosomal protein Ninein localizes asymmetrically to stem cell centrosomes but is not required for normal development, behavior, or DNA damage response in Drosophila.
Molecular biology of the cell
Molecular Dynamics Simulations Reveal the Mechanisms of Allosteric Activation of Hsp90 by Designed Ligands.
Scientific reports
Structure of ?-tubulin small complex based on a cryo-EM map, chemical cross-links, and a remotely related structure.
Journal of structural biology
Clinical validation of Anyplex™ II HPV HR Detection according to the guidelines for HPV test requirements for cervical cancer screening.
Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology
Activity-Independent Discovery of Secondary Metabolites Using Chemical Elicitation and Cheminformatic Inference.
ACS chemical biology
Asynchronous data acquisition and on-the-fly analysis of dose fractionated cryoEM images by UCSFImage.
Journal of structural biology
Evaluation of super-resolution performance of the K2 electron-counting camera using 2D crystals of aquaporin-0.
Journal of structural biology
Activation of Hsp90 Enzymatic Activity and Conformational Dynamics through Rationally Designed Allosteric Ligands.
Chemistry (Weinheim an der Bergstrasse, Germany)
Interaction of CK1d with ?TuSC ensures proper microtubule assembly and spindle positioning.
Molecular biology of the cell
MRC2014: Extensions to the MRC format header for electron cryo-microscopy and tomography.
Journal of structural biology
Ring closure activates yeast ?TuRC for species-specific microtubule nucleation.
Nature structural & molecular biology
Elucidating the mechanism of substrate recognition by the bacterial Hsp90 molecular chaperone.
Journal of molecular biology
Sparse labeling of proteins: structural characterization from long range constraints.
Journal of magnetic resonance (San Diego, Calif. : 1997)
Microarray-based screening of heat shock protein inhibitors.
Journal of biotechnology
The structure and assembly mechanism of a novel three-stranded tubulin filament that centers phage DNA.
Structure (London, England : 1993)
Bacterial tubulin TubZ-Bt transitions between a two-stranded intermediate and a four-stranded filament upon GTP hydrolysis.
Proceedings of the National Academy of Sciences of the United States of America
The free energy profile of tubulin straight-bent conformational changes, with implications for microtubule assembly and drug discovery.
PLoS computational biology
Structural asymmetry in the closed state of mitochondrial Hsp90 (TRAP1) supports a two-step ATP hydrolysis mechanism.
Molecular cell
Amorphous no more: subdiffraction view of the pericentriolar material architecture.
Trends in cell biology
A new approach for the laboratory culture of the fathead minnow, Pimephales promelas.
Environmental toxicology and chemistry
Cell-cycle regulation of formin-mediated actin cable assembly.
Proceedings of the National Academy of Sciences of the United States of America
High-resolution restoration of 3D structures from widefield images with extreme low signal-to-noise-ratio.
Proceedings of the National Academy of Sciences of the United States of America
The Aptima HPV assay fulfills the cross-sectional clinical and reproducibility criteria of international guidelines for human papillomavirus test requirements for cervical screening.
Journal of clinical microbiology
Influence of electron dose rate on electron counting images recorded with the K2 camera.
Journal of structural biology
Electron counting and beam-induced motion correction enable near-atomic-resolution single-particle cryo-EM.
Nature methods
Uncovering a region of heat shock protein 90 important for client binding in E. coli and chaperone function in yeast.
Molecular cell
The bacterial actin MamK: in vitro assembly behavior and filament architecture.
The Journal of biological chemistry
A method for integrative structure determination of protein-protein complexes.
Bioinformatics (Oxford, England)
Subdiffraction-resolution fluorescence microscopy reveals a domain of the centrosome critical for pericentriolar material organization.
Nature cell biology
The conserved arginine 380 of Hsp90 is not a catalytic residue, but stabilizes the closed conformation required for ATP hydrolysis.
Protein science : a publication of the Protein Society
Functional modulation of a protein folding landscape via side-chain distortion.
Proceedings of the National Academy of Sciences of the United States of America
Interferometer-based structured-illumination microscopy utilizing complementary phase relationship through constructive and destructive image detection by two cameras.
Journal of microscopy
Effect of depth dependent spherical aberrations in 3D structured illumination microscopy.
Optics express
Three-dimensional structure of basal body triplet revealed by electron cryo-tomography.
The EMBO journal
Cross-monomer substrate contacts reposition the Hsp90 N-terminal domain and prime the chaperone activity.
Journal of molecular biology
Microtubule nucleation by ?-tubulin complexes.
Nature reviews. Molecular cell biology
A small molecule that preferentially binds the closed conformation of Hsp90.
Bioorganic & medicinal chemistry letters
Crystal structure of ?-tubulin complex protein GCP4 provides insight into microtubule nucleation.
Nature structural & molecular biology
Client-loading conformation of the Hsp90 molecular chaperone revealed in the cryo-EM structure of the human Hsp90:Hop complex.
Molecular cell
Sas-4 provides a scaffold for cytoplasmic complexes and tethers them in a centrosome.
Nature communications
Substrate binding drives large-scale conformational changes in the Hsp90 molecular chaperone.
Molecular cell
A distributed multi-GPU system for high speed electron microscopic tomographic reconstruction.
Ultramicroscopy
Conformational dynamics of the molecular chaperone Hsp90.
Quarterly reviews of biophysics
Architecture and assembly of a divergent member of the ParM family of bacterial actin-like proteins.
The Journal of biological chemistry
Condensed mitotic chromosome structure at nanometer resolution using PALM and EGFP- histones.
PloS one
Fast live simultaneous multiwavelength four-dimensional optical microscopy.
Proceedings of the National Academy of Sciences of the United States of America
Microtubule nucleating gamma-TuSC assembles structures with 13-fold microtubule-like symmetry.
Nature
High-performance iterative electron tomography reconstruction with long-object compensation using graphics processing units (GPUs).
Journal of structural biology
Conserved tertiary couplings stabilize elements in the PDZ fold, leading to characteristic patterns of domain conformational flexibility.
Protein science : a publication of the Protein Society
Unfolding simulations reveal the mechanism of extreme unfolding cooperativity in the kinetically stable alpha-lytic protease.
PLoS computational biology
Closed loop adaptive optics for microscopy without a wavefront sensor.
Proceedings of SPIE--the International Society for Optical Engineering
High-resolution wide-field microscopy with adaptive optics for spherical aberration correction and motionless focusing.
Journal of microscopy
Self-assembling SAS-6 multimer is a core centriole building block.
The Journal of biological chemistry
Automated data collection for electron microscopic tomography.
Methods in enzymology
Mechanistic studies of Sansalvamide A-amide: an allosteric modulator of Hsp90.
ACS medicinal chemistry letters
Osmolyte-induced conformational changes in the Hsp90 molecular chaperone.
Protein science : a publication of the Protein Society
On the efficiency of iterative ordered subset reconstruction algorithms for acceleration on GPUs.
Computer methods and programs in biomedicine
Grp94, the endoplasmic reticulum Hsp90, has a similar solution conformation to cytosolic Hsp90 in the absence of nucleotide.
Protein science : a publication of the Protein Society
Localization and orientation of the gamma-tubulin small complex components using protein tags as labels for single particle EM.
Journal of structural biology
The structure and assembly dynamics of plasmid actin AlfA imply a novel mechanism of DNA segregation.
Journal of bacteriology
Dual-axis target mapping and automated sequential acquisition of dual-axis EM tomographic data.
Journal of structural biology
pH-dependent conformational changes in bacterial Hsp90 reveal a Grp94-like conformation at pH 6 that is highly active in suppression of citrate synthase aggregation.
Journal of molecular biology
Fully automated, sequential tilt-series acquisition with Leginon.
Journal of structural biology
Probing the flexibility of large conformational changes in protein structures through local perturbations.
PLoS computational biology
Species-dependent ensembles of conserved conformational states define the Hsp90 chaperone ATPase cycle.
Molecular cell
Identification of new, well-populated amino-acid sidechain rotamers involving hydroxyl-hydrogen atoms and sulfhydryl-hydrogen atoms.
BMC structural biology
Integration of small-angle X-ray scattering data into structural modeling of proteins and their assemblies.
Journal of molecular biology
Subdiffraction multicolor imaging of the nuclear periphery with 3D structured illumination microscopy.
Science (New York, N.Y.)
Intra- and intermonomer interactions are required to synergistically facilitate ATP hydrolysis in Hsp90.
The Journal of biological chemistry
Multiple conformations of E. coli Hsp90 in solution: insights into the conformational dynamics of Hsp90.
Structure (London, England : 1993)
Mob4 plays a role in spindle focusing in Drosophila S2 cells.
Journal of cell science
The lattice as allosteric effector: structural studies of alphabeta- and gamma-tubulin clarify the role of GTP in microtubule assembly.
Proceedings of the National Academy of Sciences of the United States of America
I5S: wide-field light microscopy with 100-nm-scale resolution in three dimensions.
Biophysical journal
Three-dimensional resolution doubling in wide-field fluorescence microscopy by structured illumination.
Biophysical journal
The structure of the gamma-tubulin small complex: implications of its architecture and flexibility for microtubule nucleation.
Molecular biology of the cell
AIDA: an adaptive image deconvolution algorithm with application to multi-frame and three-dimensional data.
Journal of the Optical Society of America. A, Optics, image science, and vision
Mesophile versus thermophile: insights into the structural mechanisms of kinetic stability.
Journal of molecular biology
Modelling the application of adaptive optics to wide-field microscope live imaging.
Journal of microscopy
Structural and mechanistic exploration of acid resistance: kinetic stability facilitates evolution of extremophilic behavior.
Journal of molecular biology
Automated acquisition of electron microscopic random conical tilt sets.
Journal of structural biology
Tunable instability mechanisms of polymer thin films by molecular self-assembly.
Langmuir : the ACS journal of surfaces and colloids
Electron energy filtering significantly improves amplitude contrast of frozen-hydrated protein at 300kV.
Journal of structural biology
Subangstrom crystallography reveals that short ionic hydrogen bonds, and not a His-Asp low-barrier hydrogen bond, stabilize the transition state in serine protease catalysis.
Journal of the American Chemical Society
UCSF tomography: an integrated software suite for real-time electron microscopic tomographic data collection, alignment, and reconstruction.
Journal of structural biology
Intramolecular signaling pathways revealed by modeling anisotropic thermal diffusion.
Journal of molecular biology
A novel 3D wavelet-based filter for visualizing features in noisy biological data.
Journal of microscopy
Comprehensive analysis of protein folding activation thermodynamics reveals a universal behavior violated by kinetically stable proteases.
Journal of molecular biology
Use of surface affinity enrichment and cryo-embedding to prepare in vitro reconstituted mitotic chromosomes for EM tomography.
Ultramicroscopy
Suite of three protein crystallography beamlines with single superconducting bend magnet as the source.
Journal of synchrotron radiation
Phase-retrieved pupil functions in wide-field fluorescence microscopy.
Journal of microscopy
Spindle pole body duplication in fission yeast occurs at the G1/S boundary but maturation is blocked until exit from S by an event downstream of cdc10+.
Molecular biology of the cell
An improved strategy for automated electron microscopic tomography.
Journal of structural biology
The crystal structure of the carboxy-terminal dimerization domain of htpG, the Escherichia coli Hsp90, reveals a potential substrate binding site.
Structure (London, England : 1993)
The 0.83 A resolution crystal structure of alpha-lytic protease reveals the detailed structure of the active site and identifies a source of conformational strain.
Journal of molecular biology
Disabling the folding catalyst is the last critical step in alpha-lytic protease folding.
Protein science : a publication of the Protein Society
The folding landscape of Streptomyces griseus protease B reveals the energetic costs and benefits associated with evolving kinetic stability.
Protein science : a publication of the Protein Society
Interdependent folding of the N- and C-terminal domains defines the cooperative folding of alpha-lytic protease.
Biochemistry
Phase retrieval for high-numerical-aperture optical systems.
Optics letters
Assessment of the role of computations in structural biology.
Advances in protein chemistry
Clathrin light and heavy chain interface: alpha-helix binding superhelix loops via critical tryptophans.
The EMBO journal
Centriole duplication: centrin in on answers?
Current biology : CB
Structural characterization of a subtype-selective ligand reveals a novel mode of estrogen receptor antagonism.
Nature structural biology
Structural studies of the scrapie prion protein by electron crystallography.
Proceedings of the National Academy of Sciences of the United States of America
Binding mode prediction for a flexible ligand in a flexible pocket using multi-conformation simulated annealing pseudo crystallographic refinement.
Journal of molecular biology
GCP5 and GCP6: two new members of the human gamma-tubulin complex.
Molecular biology of the cell
Enzyme specificity under dynamic control II: Principal component analysis of alpha-lytic protease using global and local solvent boundary conditions.
Protein science : a publication of the Protein Society
Chromosome elasticity and mitotic polar ejection force measured in living Drosophila embryos by four-dimensional microscopy-based motion analysis.
Current biology : CB
Gamma-tubulin complexes and microtubule nucleation.
Current opinion in structural biology
Computational adaptive optics for live three-dimensional biological imaging.
Proceedings of the National Academy of Sciences of the United States of America
Reconstitution of centrosome microtubule nucleation in Drosophila.
Methods in cell biology
Estrogen receptor pathways to AP-1.
The Journal of steroid biochemistry and molecular biology
Structure of the gamma-tubulin ring complex: a template for microtubule nucleation.
Nature cell biology
Two energetically disparate folding pathways of alpha-lytic protease share a single transition state.
Nature structural biology
Evidence for the coincident initiation of homolog pairing and synapsis during the telomere-clustering (bouquet) stage of meiotic prophase.
Journal of cell science
Direct measurement of interfacial curvature distributions in a bicontinuous block copolymer morphology.
Physical review letters
Oestrogen receptor function at classical and alternative response elements.
Novartis Foundation symposium
Kinetic stability as a mechanism for protease longevity.
Proceedings of the National Academy of Sciences of the United States of America
I5M: 3D widefield light microscopy with better than 100 nm axial resolution.
Journal of microscopy
Enzyme specificity under dynamic control: a normal mode analysis of alpha-lytic protease.
Journal of molecular biology
Structure of alpha-lytic protease complexed with its pro region.
Nature structural biology
Relationship between enzyme specificity and the backbone dynamics of free and inhibited alpha-lytic protease.
Biochemistry
Homologous chromosome pairing in Drosophila melanogaster proceeds through multiple independent initiations.
The Journal of cell biology
Activation of a retroviral membrane fusion protein: soluble receptor-induced liposome binding of the ALSV envelope glycoprotein.
The Journal of cell biology
Interphase chromosomes undergo constrained diffusional motion in living cells.
Current biology : CB
Practical image restoration of thick biological specimens using multiple focus levels in transmission electron microscopy.
Journal of structural biology
Alterations in chemical shifts and exchange broadening upon peptide boronic acid inhibitor binding to alpha-lytic protease.
Journal of biomolecular NMR
Conformational substates in enzyme mechanism: the 120 K structure of alpha-lytic protease at 1.5 A resolution.
Protein science : a publication of the Protein Society
Telomeres cluster de novo before the initiation of synapsis: a three-dimensional spatial analysis of telomere positions before and during meiotic prophase.
The Journal of cell biology
Inhibition of alpha-lytic protease by pro region C-terminal steric occlusion of the active site.
Biochemistry
Optimal strategies for imaging thick biological specimens: exit wavefront reconstruction and energy-filtered imaging.
Journal of microscopy
Specific interactions of chromatin with the nuclear envelope: positional determination within the nucleus in Drosophila melanogaster.
Molecular biology of the cell
Dispersion, aberration and deconvolution in multi-wavelength fluorescence images.
Journal of microscopy
Time-resolved, in vivo studies of mitotic spindle formation and nuclear lamina breakdown in Drosophila early embryos.
Journal of cell science
IVE (Image Visualization Environment): a software platform for all three-dimensional microscopy applications.
Journal of structural biology
Toward fully automated high-resolution electron tomography.
Journal of structural biology
Kinetic and structural characterization of mutations of glycine 216 in alpha-lytic protease: a new target for engineering substrate specificity.
Journal of molecular biology
A three-dimensional structural dissection of Drosophila polytene chromosomes.
The Journal of cell biology
Three-dimensional structural characterization of centrosomes from early Drosophila embryos.
The Journal of cell biology
Functional linkage between the active site of alpha-lytic protease and distant regions of structure: scanning alanine mutagenesis of a surface loop affects activity and substrate specificity.
Journal of molecular biology
Mechanism of image formation for thick biological specimens: exit wavefront reconstruction and electron energy-loss spectroscopic imaging.
Journal of microscopy
Influenza hemagglutinin: kinetic control of protein function.
Structure (London, England : 1993)
Human apolipoprotein E. Role of arginine 61 in mediating the lipoprotein preferences of the E3 and E4 isoforms.
The Journal of biological chemistry
Salt bridge relay triggers defective LDL receptor binding by a mutant apolipoprotein.
Structure (London, England : 1993)
Kinetics versus thermodynamics in protein folding.
Biochemistry
Cryo automated electron tomography: towards high-resolution reconstructions of plastic-embedded structures.
Journal of microscopy
The three-dimensional architecture of chromatin in situ: electron tomography reveals fibers composed of a continuously variable zig-zag nucleosomal ribbon.
The Journal of cell biology
The role of pro regions in protein folding.
Current opinion in cell biology
PRISM: application to the solution of two protein structures.
Acta crystallographica. Section D, Biological crystallography
PRISM: topologically constrained phased refinement for macromolecular crystallography.
Acta crystallographica. Section D, Biological crystallography
To fold or not to fold....
Science (New York, N.Y.)
Chromosome structure inside the nucleus.
Current opinion in cell biology
Modeling side-chain conformation for homologous proteins using an energy-based rotamer search.
Journal of molecular biology
The onset of homologous chromosome pairing during Drosophila melanogaster embryogenesis.
The Journal of cell biology
Uniqueness and the ab initio phase problem in macromolecular crystallography.
Acta crystallographica. Section D, Biological crystallography
Automated microscopy for electron tomography.
Ultramicroscopy
An extended view of nuclear lamin structure, function, and dynamics.
Seminars in cell biology
Correct folding of alpha-lytic protease is required for its extracellular secretion from Escherichia coli.
The Journal of cell biology
Computational method for the design of enzymes with altered substrate specificity.
Journal of molecular biology
Three-dimensional structure of the LDL receptor-binding domain of human apolipoprotein E.
Science (New York, N.Y.)
Drosophila gastrulation: analysis of cell shape changes in living embryos by three-dimensional fluorescence microscopy.
Development (Cambridge, England)
Three-dimensional multiple-wavelength fluorescence microscopy for the structural analysis of biological phenomena.
Seminars in cell biology
Crystal structures of alpha-lytic protease complexes with irreversibly bound phosphonate esters.
Biochemistry
Mutational remodeling of enzyme specificity.
Methods in enzymology
Temporal and spatial coordination of chromosome movement, spindle formation, and nuclear envelope breakdown during prometaphase in Drosophila melanogaster embryos.
The Journal of cell biology
Determination of three-dimensional imaging properties of a light microscope system. Partial confocal behavior in epifluorescence microscopy.
Biophysical journal
Structural analysis of specificity: alpha-lytic protease complexes with analogues of reaction intermediates.
Biochemistry
Direct cell lineage analysis in Drosophila melanogaster by time-lapse, three-dimensional optical microscopy of living embryos.
The Journal of cell biology
Analysis of prepro-alpha-lytic protease expression in Escherichia coli reveals that the pro region is required for activity.
Journal of bacteriology
Fluorescence microscopy in three dimensions.
Methods in cell biology
Solid-state imagers for microscopy.
Methods in cell biology
Tilted view reconstruction in optical microscopy. Three-dimensional reconstruction of Drosophila melanogaster embryo nuclei.
Biophysical journal
Crystallization and preliminary X-ray diffraction studies on the amino-terminal (receptor-binding) domain of human apolipoprotein E3 from serum very low density lipoproteins.
Journal of molecular biology
Three-dimensional light microscopy of diploid Drosophila chromosomes.
Cell motility and the cytoskeleton
The use of a charge-coupled device for quantitative optical microscopy of biological structures.
Science (New York, N.Y.)
[Hepatonephritis caused by piroxicam].
Gastroenterologie clinique et biologique
A three-dimensional approach to mitotic chromosome structure: evidence for a complex hierarchical organization.
The Journal of cell biology
Quantitative analysis of nucleic acids, proteins, and viruses by Raman band deconvolution.
Biophysical journal
[Anorectal manifestations of sexually transmissible diseases. Kaposi's sarcoma].
Annales de gastroenterologie et d'hepatologie
Optical sectioning microscopy: cellular architecture in three dimensions.
Annual review of biophysics and bioengineering
Spatial organization of the Drosophila nucleus: a three-dimensional cytogenetic study.
Journal of cell science. Supplement
A least-squares method for determining structure factors in three-dimensional tilted-view reconstructions.
Journal of molecular biology
[Syndrome of inappropriate antidiuretic hormone secretion. A report of two cases with hyponatremia and hypouricemia].
La semaine des hopitaux : organe fonde par l'Association d'enseignement medical des hopitaux de Paris
Linking regions between helices in bacteriorhodopsin revealed.
Biophysical journal
Studies on the phosphorylation and synthesis of type I regulatory subunit of cyclic AMP-dependent protein kinase in intact S49 mouse lymphoma cells.
The Journal of biological chemistry
Turnover of regulatory subunit of cyclic AMP-dependent protein kinase in S49 mouse lymphoma cells. Regulation by catalytic subunit and analogs of cyclic AMP.
The Journal of biological chemistry
Quantitative analysis of electrophoretograms: a mathematical approach to super-resolution.
Analytical biochemistry
[Hemorrhoids. Physiopathogenic concepts and approach to a rational treatment].
Gastroenterologie clinique et biologique
Structure determination of asymmetric membrane profiles using an iterative Fourier method.
Biophysical journal
[Gastrointestinal haemorrhage in the cirrhotic. The value of injectable posterior pituitary extract (author's transl)].
La Nouvelle presse medicale
[The excision of villous tumours of the rectum via a trans-sphincterian anal approach (author's transl)].
La Nouvelle presse medicale
[Secondary melanomas of the liver. Appropos of 3 cases with hepatic symptomatology].
Medecine & chirurgie digestives
Structural studies of a membrane-bound acetylcholine receptor from Torpedo californica.
Journal of molecular biology
[Systemic amyloidosis and multiple myeloma. Report of a case with the association of light chain myeloma and gastric plasmacytosis (author's transl)].
Gastroenterologie clinique et biologique