
Professor
Department of Biochemistry and Biophysics
+1 415 987-7535
We seek to understand molecular mechanisms of certain key biological processes, as well as signal transduction between processes at the level of protein structure, dynamics, and mechanism. Molecular cloning, expression, site-directed mutagenesis, and strong focus onto biochemistry are a key part of the arsenal for defining molecular mechanisms. We focus on the questions of how signals cross membranes, and how membrane proteins and membrane channels work at the level of atomic structure. We also focus on how the signal recognition particle (SRP) directs membrane and secreted proteins to the membrane.
Publications
Substrate recognition and allosteric regulation of synaptic vesicle glutamate transporter VGLUT2.
Nature structural & molecular biology
Structure and inhibition mechanisms of Mycobacterium tuberculosis essential transporter efflux protein A.
Nature communications
Structural basis of disease mutation and substrate recognition by the human SLC2A9 transporter.
Proceedings of the National Academy of Sciences of the United States of America
Structure and inhibition mechanisms of Mycobacterium tuberculosis essential transporter efflux protein A.
bioRxiv : the preprint server for biology
AcrIF11 is a potent CRISPR-specific ADP-ribosyltransferase encoded by phage and plasmid.
bioRxiv : the preprint server for biology
Biochemical characterization of a high affinity phosphate transporter (PiPT) from root endophyte fungus Piriformospora indica.
Protein expression and purification
Structural and mechanistic insights into a lysosomal membrane enzyme HGSNAT involved in Sanfilippo syndrome.
Nature communications
Structural Basis of Aquaporin-4 Autoantibody Binding in Neuromyelitis Optica.
bioRxiv : the preprint server for biology
Kinetic Network Modeling with Molecular Simulation Inputs: A Proton-Coupled Phosphate Symporter.
Biophysical journal
Structural basis of prostaglandin efflux by MRP4.
Nature structural & molecular biology
Substrate recognition and proton coupling by a bacterial member of solute carrier family 17.
The Journal of biological chemistry
Structure and dynamics of the essential endogenous mycobacterial polyketide synthase Pks13.
Nature structural & molecular biology
Structure and dynamics of the essential endogenous mycobacterial polyketide synthase Pks13.
bioRxiv : the preprint server for biology
Diversity of function and mechanism in a family of organic anion transporters.
Current opinion in structural biology
Molecular basis of multistep voltage activation in plant two-pore channel 1.
Proceedings of the National Academy of Sciences of the United States of America
Allosteric Inhibition of a Vesicular Glutamate Transporter by an Isoform-Specific Antibody.
Biochemistry
Key computational findings reveal proton transfer as driving the functional cycle in the phosphate transporter PiPT.
Proceedings of the National Academy of Sciences of the United States of America
CryoEM and AI reveal a structure of SARS-CoV-2 Nsp2, a multifunctional protein involved in key host processes.
Research square
CryoEM and AI reveal a structure of SARS-CoV-2 Nsp2, a multifunctional protein involved in key host processes.
bioRxiv : the preprint server for biology
Structural basis for Clostridium perfringens enterotoxin targeting of claudins at tight junctions in mammalian gut.
Proceedings of the National Academy of Sciences of the United States of America
Caught in Action: X-ray Structure of Thymidylate Synthase with Noncovalent Intermediate Analog.
Biochemistry
Highlighting membrane protein structure and function: A celebration of the Protein Data Bank.
The Journal of biological chemistry
Structural and mechanistic basis of the EMC-dependent biogenesis of distinct transmembrane clients.
eLife
Comparative host-coronavirus protein interaction networks reveal pan-viral disease mechanisms.
Science (New York, N.Y.)
Ion transport and regulation in a synaptic vesicle glutamate transporter.
Science (New York, N.Y.)
Resting state structure of the hyperdepolarization activated two-pore channel 3.
Proceedings of the National Academy of Sciences of the United States of America
Claudin-9 structures reveal mechanism for toxin-induced gut barrier breakdown.
Proceedings of the National Academy of Sciences of the United States of America
Structures suggest a mechanism for energy coupling by a family of organic anion transporters.
PLoS biology
High-pressure, high-temperature molecular doping of nanodiamond.
Science advances
High-Throughput Nano-Scale Characterization of Membrane Proteins Using Fluorescence-Detection Size-Exclusion Chromatography.
Methods in molecular biology (Clifton, N.J.)
Structural basis for activation of voltage sensor domains in an ion channel TPC1.
Proceedings of the National Academy of Sciences of the United States of America
CRL4AMBRA1 targets Elongin C for ubiquitination and degradation to modulate CRL5 signaling.
The EMBO journal
Crystal Structure of a ligand-bound LacY-Nanobody Complex.
Proceedings of the National Academy of Sciences of the United States of America
A Single Mutation Traps a Half-Sites Reactive Enzyme in Midstream, Explaining Asymmetry in Hydride Transfer.
Biochemistry
On the structure and mechanism of two-pore channels.
The FEBS journal
Crystal structure and mechanistic basis of a functional homolog of the antigen transporter TAP.
Proceedings of the National Academy of Sciences of the United States of America
The Preserved HTH-Docking Cleft of HIV-1 Integrase Is Functionally Critical.
Structure (London, England : 1993)
Structure of Bor1 supports an elevator transport mechanism for SLC4 anion exchangers.
Proceedings of the National Academy of Sciences of the United States of America
Mechanism of inhibition of human glucose transporter GLUT1 is conserved between cytochalasin B and phenylalanine amides.
Proceedings of the National Academy of Sciences of the United States of America
Integral Membrane Protein Expression in Saccharomyces cerevisiae.
Methods in molecular biology (Clifton, N.J.)
Small-Scale Screening to Large-Scale Over-Expression of Human Membrane Proteins for Structural Studies.
Methods in molecular biology (Clifton, N.J.)
Crystal Structure of the Human tRNA m(1)A58 Methyltransferase-tRNA(3)(Lys) Complex: Refolding of Substrate tRNA Allows Access to the Methylation Target.
Journal of molecular biology
Fungal association and utilization of phosphate by plants: success, limitations, and future prospects.
Frontiers in microbiology
Structure of LacY with an a-substituted galactoside: Connecting the binding site to the protonation site.
Proceedings of the National Academy of Sciences of the United States of America
Hotspots in an obligate homodimeric anticancer target. Structural and functional effects of interfacial mutations in human thymidylate synthase.
Journal of medicinal chemistry
Alanine mutants of the interface residues of human thymidylate synthase decode key features of the binding mode of allosteric anticancer peptides.
Journal of medicinal chemistry
General qPCR and Plate Reader Methods for Rapid Optimization of Membrane Protein Purification and Crystallization Using Thermostability Assays.
Current protocols in protein science
Tetra detector analysis of membrane proteins.
Current protocols in protein science
Structure of a new DNA-binding domain which regulates pathogenesis in a wide variety of fungi.
Proceedings of the National Academy of Sciences of the United States of America
Architecture of a single membrane spanning cytochrome P450 suggests constraints that orient the catalytic domain relative to a bilayer.
Proceedings of the National Academy of Sciences of the United States of America
Structure of sugar-bound LacY.
Proceedings of the National Academy of Sciences of the United States of America
The mechanism of pseudouridine synthases from a covalent complex with RNA, and alternate specificity for U2605 versus U2604 between close homologs.
Nucleic acids research
Structures of intermediate transport states of ZneA, a Zn(II)/proton antiporter.
Proceedings of the National Academy of Sciences of the United States of America
Development and binding mode assessment of N-[4-[2-propyn-1-yl[(6S)-4,6,7,8-tetrahydro-2-(hydroxymethyl)-4-oxo-3H-cyclopenta[g]quinazolin-6-yl]amino]benzoyl]-l-?-glutamyl-D-glutamic acid (BGC 945), a novel thymidylate synthase inhibitor that targets tumor
Journal of medicinal chemistry
In human pseudouridine synthase 1 (hPus1), a C-terminal helical insert blocks tRNA from binding in the same orientation as in the Pus1 bacterial homologue TruA, consistent with their different target selectivities.
Journal of molecular biology
Mg2+ binds to the surface of thymidylate synthase and affects hydride transfer at the interior active site.
Journal of the American Chemical Society
Coordinating the impact of structural genomics on the human a-helical transmembrane proteome.
Nature structural & molecular biology
Inhibitor of ovarian cancer cells growth by virtual screening: a new thiazole derivative targeting human thymidylate synthase.
Journal of medicinal chemistry
A remote mutation affects the hydride transfer by disrupting concerted protein motions in thymidylate synthase.
Journal of the American Chemical Society
LpxI structures reveal how a lipid A precursor is synthesized.
Nature structural & molecular biology
The structure of Enterococcus faecalis thymidylate synthase provides clues about folate bacterial metabolism.
Acta crystallographica. Section D, Biological crystallography
Aquaporin 4-specific T cells in neuromyelitis optica exhibit a Th17 bias and recognize Clostridium ABC transporter.
Annals of neurology
Fabs enable single particle cryoEM studies of small proteins.
Structure (London, England : 1993)
Overexpressing human membrane proteins in stably transfected and clonal human embryonic kidney 293S cells.
Nature protocols
Rapid identification of recombinant Fabs that bind to membrane proteins.
Methods (San Diego, Calif.)
Efficient expression screening of human membrane proteins in transiently transfected Human Embryonic Kidney 293S cells.
Methods (San Diego, Calif.)
Membranes.
Current opinion in structural biology
EspR, a key regulator of Mycobacterium tuberculosis virulence, adopts a unique dimeric structure among helix-turn-helix proteins.
Proceedings of the National Academy of Sciences of the United States of America
New tools in membrane protein determination.
F1000 biology reports
Lateral opening of a translocon upon entry of protein suggests the mechanism of insertion into membranes.
Proceedings of the National Academy of Sciences of the United States of America
Structural context shapes the aquaporin selectivity filter.
Proceedings of the National Academy of Sciences of the United States of America
Unlocking the eukaryotic membrane protein structural proteome.
Current opinion in structural biology
Metal-induced conformational changes in ZneB suggest an active role of membrane fusion proteins in efflux resistance systems.
Proceedings of the National Academy of Sciences of the United States of America
Function of human Rh based on structure of RhCG at 2.1 A.
Proceedings of the National Academy of Sciences of the United States of America
Comparison of human solute carriers.
Protein science : a publication of the Protein Society
Overexpression and purification of integral membrane proteins in yeast.
Methods in enzymology
A survey of integral alpha-helical membrane proteins.
Journal of structural and functional genomics
Catalytically-active complex of HIV-1 integrase with a viral DNA substrate binds anti-integrase drugs.
Proceedings of the National Academy of Sciences of the United States of America
Crystal structure of human aquaporin 4 at 1.8 A and its mechanism of conductance.
Proceedings of the National Academy of Sciences of the United States of America
Crystal structure of an RluF-RNA complex: a base-pair rearrangement is the key to selectivity of RluF for U2604 of the ribosome.
Journal of molecular biology
2007 annual progress report synopsis of the Center for Structures of Membrane Proteins.
Journal of structural and functional genomics
A general protocol for the crystallization of membrane proteins for X-ray structural investigation.
Nature protocols
Selecting optimum eukaryotic integral membrane proteins for structure determination by rapid expression and solubilization screening.
Journal of molecular biology
Ratiocinative screen of eukaryotic integral membrane protein expression and solubilization for structure determination.
Journal of structural and functional genomics
Michael A. Raftery (1936-2007)--the first enzyme mechanism, sequential cooperativity, and the nicotinic acetylcholine receptor defined.
Protein science : a publication of the Protein Society
Crystal structure of bovine mitochondrial factor B at 0.96-A resolution.
Proceedings of the National Academy of Sciences of the United States of America
Crystal structure of the aquaglyceroporin PfAQP from the malarial parasite Plasmodium falciparum.
Nature structural & molecular biology
Structure of a TrmA-RNA complex: A consensus RNA fold contributes to substrate selectivity and catalysis in m5U methyltransferases.
Proceedings of the National Academy of Sciences of the United States of America
Structural and biochemical insights into the dicing mechanism of mouse Dicer: a conserved lysine is critical for dsRNA cleavage.
Proceedings of the National Academy of Sciences of the United States of America
TARP auxiliary subunits switch AMPA receptor antagonists into partial agonists.
Science (New York, N.Y.)
X-ray structures of the signal recognition particle receptor reveal targeting cycle intermediates.
PloS one
X-ray crystallographic and NMR studies of protein-protein and protein-nucleic acid interactions involving the KH domains from human poly(C)-binding protein-2.
RNA (New York, N.Y.)
Cell-free complements in vivo expression of the E. coli membrane proteome.
Protein science : a publication of the Protein Society
Crystal structure of the third KH domain of human poly(C)-binding protein-2 in complex with a C-rich strand of human telomeric DNA at 1.6 A resolution.
Nucleic acids research
Structural basis of aquaporin inhibition by mercury.
Journal of molecular biology
The crystal structure of E. coli rRNA pseudouridine synthase RluE.
Journal of molecular biology
Transmembrane transporters: an open and closed case.
Proceedings of the National Academy of Sciences of the United States of America
Inhibitory complex of the transmembrane ammonia channel, AmtB, and the cytosolic regulatory protein, GlnK, at 1.96 A.
Proceedings of the National Academy of Sciences of the United States of America
The Amt/MEP/Rh family: structure of AmtB and the mechanism of ammonia gas conduction.
Physiology (Bethesda, Md.)
Substrate recognition by RNA 5-methyluridine methyltransferases and pseudouridine synthases: a structural perspective.
The Journal of biological chemistry
Pyrrolidine carboxamides as a novel class of inhibitors of enoyl acyl carrier protein reductase from Mycobacterium tuberculosis.
Journal of medicinal chemistry
The structure of a ketoreductase determines the organization of the beta-carbon processing enzymes of modular polyketide synthases.
Structure (London, England : 1993)
On the mechanism of sensing unfolded protein in the endoplasmic reticulum.
Proceedings of the National Academy of Sciences of the United States of America
Structural basis for conductance by the archaeal aquaporin AqpM at 1.68 A.
Proceedings of the National Academy of Sciences of the United States of America
The structure of Cryptococcus neoformans thymidylate synthase suggests strategies for using target dynamics for species-specific inhibition.
Acta crystallographica. Section D, Biological crystallography
Crystal structure of the first KH domain of human poly(C)-binding protein-2 in complex with a C-rich strand of human telomeric DNA at 1.7 A.
The Journal of biological chemistry
Targeting proteins to membranes: structure of the signal recognition particle.
Current opinion in structural biology
De novo design of an IL-4 antagonist and its structure at 1.9 A.
Proceedings of the National Academy of Sciences of the United States of America
Unraveling the interface of signal recognition particle and its receptor by using chemical cross-linking and tandem mass spectrometry.
Proceedings of the National Academy of Sciences of the United States of America
The channel architecture of aquaporin 0 at a 2.2-A resolution.
Proceedings of the National Academy of Sciences of the United States of America
Mechanism of ammonia transport by Amt/MEP/Rh: structure of AmtB at 1.35 A.
Science (New York, N.Y.)
An antibiotic factory caught in action.
Nature structural & molecular biology
Redox reactions of the iron-sulfur cluster in a ribosomal RNA methyltransferase, RumA: optical and EPR studies.
The Journal of biological chemistry
Mechanistic diversity of cytokine receptor signaling across cell membranes.
Science's STKE : signal transduction knowledge environment
Crystal structure of RumA, an iron-sulfur cluster containing E. coli ribosomal RNA 5-methyluridine methyltransferase.
Structure (London, England : 1993)
The first structure of an RNA m5C methyltransferase, Fmu, provides insight into catalytic mechanism and specific binding of RNA substrate.
Structure (London, England : 1993)
Crystal structure of an Acyl-ACP dehydrogenase from the FK520 polyketide biosynthetic pathway: insights into extender unit biosynthesis.
Journal of molecular biology
Water and glycerol permeation through the glycerol channel GlpF and the aquaporin family.
Journal of synchrotron radiation
Selectivity and conductance among the glycerol and water conducting aquaporin family of channels.
FEBS letters
Structure of tRNA pseudouridine synthase TruB and its RNA complex: RNA recognition through a combination of rigid docking and induced fit.
Proceedings of the National Academy of Sciences of the United States of America
Phylogenetic classification of protozoa based on the structure of the linker domain in the bifunctional enzyme, dihydrofolate reductase-thymidylate synthase.
The Journal of biological chemistry
Glycerol facilitator GlpF and the associated aquaporin family of channels.
Current opinion in structural biology
The structural roles of conserved Pro196, Pro197 and His199 in the mechanism of thymidylate synthase.
Protein engineering
Crystal structure and molecular modeling of 17-DMAG in complex with human Hsp90.
Chemistry & biology
Structure of human pro-chymase: a model for the activating transition of granule-associated proteases.
Biochemistry
The only active mutant of thymidylate synthase D169, a residue far from the site of methyl transfer, demonstrates the exquisite nature of enzyme specificity.
Protein engineering
Catalysis, specificity, and ACP docking site of Streptomyces coelicolor malonyl-CoA:ACP transacylase.
Structure (London, England : 1993)
Conformational dynamics along an enzymatic reaction pathway: thymidylate synthase, "the movie".
Biochemistry
The crystal structure of dihydrofolate reductase-thymidylate synthase from Cryptosporidium hominis reveals a novel architecture for the bifunctional enzyme.
The Journal of eukaryotic microbiology
The glycerol facilitator GlpF its aquaporin family of channels, and their selectivity.
Advances in protein chemistry
Crystal structure of the priming beta-ketosynthase from the R1128 polyketide biosynthetic pathway.
Structure (London, England : 1993)
Structural basis for mobility in the 1.1 A crystal structure of the NG domain of Thermus aquaticus Ffh.
Journal of molecular biology
Structure-based studies on species-specific inhibition of thymidylate synthase.
Biochimica et biophysica acta
Control of the selectivity of the aquaporin water channel family by global orientational tuning.
Science (New York, N.Y.)
Encoding Selectivity of a Transmembrane Channel.
TheScientificWorldJournal
Characterization of the 23 S ribosomal RNA m5U1939 methyltransferase from Escherichia coli.
The Journal of biological chemistry
The structure of GlpF, a glycerol conducting channel.
Novartis Foundation symposium
Crystal structure of the macrocycle-forming thioesterase domain of the erythromycin polyketide synthase: versatility from a unique substrate channel.
Proceedings of the National Academy of Sciences of the United States of America
Multi-targeted antifolates aimed at avoiding drug resistance form covalent closed inhibitory complexes with human and Escherichia coli thymidylate synthases.
Journal of molecular biology
Predicting and harnessing protein flexibility in the design of species-specific inhibitors of thymidylate synthase.
Chemistry & biology
Atomic structure of a glycerol channel and implications for substrate permeation in aqua(glycero)porins.
FEBS letters
Structure of Cry2Aa suggests an unexpected receptor binding epitope.
Structure (London, England : 1993)
Approaches to solving the rigid receptor problem by identifying a minimal set of flexible residues during ligand docking.
Chemistry & biology
Enzyme-catalyzed therapeutic agent (ECTA) design: activation of the antitumor ECTA compound NB1011 by thymidylate synthase.
Biochemical pharmacology
The signal recognition particle.
Annual review of biochemistry
Structure of a glycerol-conducting channel and the basis for its selectivity.
Science (New York, N.Y.)
Site-directed ligand discovery.
Proceedings of the National Academy of Sciences of the United States of America
Replacement set mutagenesis of the four phosphate-binding arginine residues of thymidylate synthase.
Protein engineering
Crystal structure of the HIV-1 integrase catalytic core and C-terminal domains: a model for viral DNA binding.
Proceedings of the National Academy of Sciences of the United States of America
Mesoscopic surfactant organization and membrane protein crystallization.
Protein science : a publication of the Protein Society
The crystal structure of thymidylate synthase from Pneumocystis carinii reveals a fungal insert important for drug design.
Journal of molecular biology
Effects of subunit occupancy on partitioning of an intermediate in thymidylate synthase mutants.
Biochemistry
Active site water molecules revealed in the 2.1 A resolution structure of a site-directed mutant of isocitrate dehydrogenase.
Journal of molecular biology
The structural basis for tRNA recognition and pseudouridine formation by pseudouridine synthase I.
Nature structural biology
Signal sequence recognition and protein targeting.
Current opinion in structural biology
Expression, purification, and structural characterization of the bacteriorhodopsin-aspartyl transcarbamylase fusion protein.
Protein expression and purification
Functional changes in the structure of the SRP GTPase on binding GDP and Mg2+GDP.
Nature structural biology
Phthalein derivatives as a new tool for selectivity in thymidylate synthase inhibition.
Journal of medicinal chemistry
Engineering a soluble extracellular erythropoietin receptor (EPObp) in Pichia pastoris to eliminate microheterogeneity, and its complex with erythropoietin.
Protein engineering
Inactivity of N229A thymidylate synthase due to water-mediated effects: isolating a late stage in methyl transfer.
Journal of molecular biology
Crystal structures of a unique thermal-stable thymidylate synthase from Bacillus subtilis.
Biochemistry
Efficiency of signalling through cytokine receptors depends critically on receptor orientation.
Nature
D221 in thymidylate synthase controls conformation change, and thereby opening of the imidazolidine.
Biochemistry
Helical protein design.
Current opinion in biotechnology
Ion-channel-forming colicins.
Current opinion in structural biology
The additivity of substrate fragments in enzyme-ligand binding.
Structure (London, England : 1993)
The separate effects of E60Q in Lactobacillus casei thymidylate synthase delineate between mechanisms for formation of intermediates in catalysis.
Protein engineering
Domain flexibility in retroviral proteases: structural implications for drug resistant mutations.
Biochemistry
Contributions of orientation and hydrogen bonding to catalysis in Asn229 mutants of thymidylate synthase.
Journal of molecular biology
A designed four helix bundle protein with native-like structure.
Nature structural biology
The discovery, characterization and crystallographically determined binding mode of an FMOC-containing inhibitor of HIV-1 protease.
Bioorganic & medicinal chemistry
Binding of the anticancer drug ZD1694 to E. coli thymidylate synthase: assessing specificity and affinity.
Structure (London, England : 1993)
A new class of HIV-1 protease inhibitor: the crystallographic structure, inhibition and chemical synthesis of an aminimide peptide isostere.
Bioorganic & medicinal chemistry
Balancing ATP in the cell.
Nature structural biology
Asparagine 229 mutants of thymidylate synthase catalyze the methylation of 3-methyl-2'-deoxyuridine 5'-monophosphate.
Biochemistry
Entropy in bi-substrate enzymes: proposed role of an alternate site in chaperoning substrate into, and products out of, thymidylate synthase.
Journal of molecular biology
The complex of the anti-cancer therapeutic, BW1843U89, with thymidylate synthase at 2.0 A resolution: implications for a new mode of inhibition.
Structure (London, England : 1993)
Contribution of a salt bridge to binding affinity and dUMP orientation to catalytic rate: mutation of a substrate-binding arginine in thymidylate synthase.
Protein engineering
Crystal structure of human thymidylate synthase: a structural mechanism for guiding substrates into the active site.
Biochemistry
Significance of structural changes in proteins: expected errors in refined protein structures.
Protein science : a publication of the Protein Society
Topochemistry for preparing ligands that dimerize receptors.
Chemistry & biology
Ion channel forming colicins.
Current opinion in structural biology
The domain structure of the ion channel-forming protein colicin Ia.
Nature structural biology
An electrostatic highway.
Nature structural biology
Water-mediated substrate/product discrimination: the product complex of thymidylate synthase at 1.83 A.
Biochemistry
Structure of the protease from simian immunodeficiency virus: complex with an irreversible nonpeptide inhibitor.
Biochemistry
Structure at 2.5 A of a designed peptide that maintains solubility of membrane proteins.
Science (New York, N.Y.)
Two-dimensional crystallization of Escherichia coli-expressed bacteriorhodopsin and its D96N variant: high resolution structural studies in projection.
Biophysical journal
Refined structures of substrate-bound and phosphate-bound thymidylate synthase from Lactobacillus casei.
Journal of molecular biology
A carboxy-terminal fragment of colicin Ia forms ion channels.
The Journal of membrane biology
Stereochemistry of a multistep/bipartite methyl transfer reaction: thymidylate synthase.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology
Structure-based discovery of inhibitors of thymidylate synthase.
Science (New York, N.Y.)
Cofactor triggers the conformational change in thymidylate synthase: implications for an ordered binding mechanism.
Biochemistry
Reversible dissociation and unfolding of the dimeric protein thymidylate synthase.
Protein science : a publication of the Protein Society
Inclusion of solvation free energy with molecular mechanics energy: alanyl dipeptide as a test case.
Protein science : a publication of the Protein Society
Epidermal growth factor (EGF) induces oligomerization of soluble, extracellular, ligand-binding domain of EGF receptor. A low resolution projection structure of the ligand-binding domain.
The Journal of biological chemistry
Crystallization of human thymidylate synthase.
Journal of molecular biology
Ion channels formed by a highly charged peptide.
Biochemistry
Wild-type and mutant bacterioopsins D85N, D96N, and R82Q: high-level expression in Escherichia coli.
Biochemistry
Resonance Raman spectra of bacteriorhodopsin mutants with substitutions at Asp-85, Asp-96, and Arg-82.
Photochemistry and photobiology
Effects of detergent environments on the photocycle of purified monomeric bacteriorhodopsin.
Biochemistry
1.59 A structure of trypsin at 120 K: comparison of low temperature and room temperature structures.
Proteins
Saturation site-directed mutagenesis of thymidylate synthase.
The Journal of biological chemistry
Regulation of an enzyme by phosphorylation at the active site.
Science (New York, N.Y.)
Regulation of isocitrate dehydrogenase by phosphorylation involves no long-range conformational change in the free enzyme.
The Journal of biological chemistry
High sensitivity electron diffraction analysis. A study of divalent cation binding to purple membrane.
Biophysical journal
Plastic adaptation toward mutations in proteins: structural comparison of thymidylate synthases.
Proteins
Prediction of homologous protein structures based on conformational searches and energetics.
Proteins
Preparative purification of functional bacteriorhodopsin by high-performance size-exclusion chromatography.
Journal of chromatography
Structure of a bacterial enzyme regulated by phosphorylation, isocitrate dehydrogenase.
Proceedings of the National Academy of Sciences of the United States of America
Structure, oligosaccharide structures, and posttranslationally modified sites of the nicotinic acetylcholine receptor.
Proceedings of the National Academy of Sciences of the United States of America
Three-dimensional structure of the nicotinic acetylcholine receptor and location of the major associated 43-kD cytoskeletal protein, determined at 22 A by low dose electron microscopy and x-ray diffraction to 12.5 A.
The Journal of cell biology
Changes in conformation upon agonist binding, and nonequivalent labeling, of the membrane-spanning regions of the nicotinic acetylcholine receptor subunits.
The Journal of biological chemistry
Purification of bacteriorhodopsin and characterization of mature and partially processed forms.
The Journal of biological chemistry
Cesium ion liquid secondary ion mass spectrometry of membrane-bound glycoproteins: structural and topological considerations of acetylcholine receptor from Torpedo californica.
Biomedical & environmental mass spectrometry
Structural and functional conservation between yeast and human 3-hydroxy-3-methylglutaryl coenzyme A reductases, the rate-limiting enzyme of sterol biosynthesis.
Molecular and cellular biology
Structural studies of alpha-bungarotoxin. 1. Sequence-specific 1H NMR resonance assignments.
Biochemistry
The three-dimensional structure of Asn102 mutant of trypsin: role of Asp102 in serine protease catalysis.
Science (New York, N.Y.)
Independent mutations at the amino terminus of a protein act as surrogate signals for mitochondrial import.
The EMBO journal
Atomic structure of thymidylate synthase: target for rational drug design.
Science (New York, N.Y.)
An archetypal molecular transducer of the nervous system: the acetylcholine receptor.
Research publications - Association for Research in Nervous and Mental Disease
Topological mapping and the ionic channel in an acetylcholine receptor.
Society of General Physiologists series
Family of G protein alpha chains: amphipathic analysis and predicted structure of functional domains.
Protein engineering
The crystal structure of alpha-bungarotoxin at 2.5 A resolution: relation to solution structure and binding to acetylcholine receptor.
Protein engineering
Cation binding sites on the projected structure of bacteriorhodopsin.
Biophysical journal
Location of terbium binding sites on acetylcholine receptor-enriched membranes.
Journal of molecular biology
Purification and crystallization of creatine kinase from rabbit skeletal muscle.
The Journal of biological chemistry
The molecular neurobiology of the acetylcholine receptor.
Annual review of neuroscience
Domain structure of 3-hydroxy-3-methylglutaryl coenzyme A reductase, a glycoprotein of the endoplasmic reticulum.
The Journal of biological chemistry
Acetylcholine receptor structure, function, and evolution.
Annual review of cell biology
Topological mapping of acetylcholine receptor: evidence for a model with five transmembrane segments and a cytoplasmic COOH-terminal peptide.
Proceedings of the National Academy of Sciences of the United States of America
Location of an extrinsic label in the primary and tertiary structure of bacteriorhodopsin.
Biophysical journal
Subunit secondary structure in filamentous viruses: predictions and observations.
Journal of biomolecular structure & dynamics
Amphipathic analysis and possible formation of the ion channel in an acetylcholine receptor.
Proceedings of the National Academy of Sciences of the United States of America
Subunit organization and structure of an acetylcholine receptor.
Cold Spring Harbor symposia on quantitative biology
Linking regions between helices in bacteriorhodopsin revealed.
Biophysical journal
Structure and function of an acetylcholine receptor.
Biophysical journal
Assembly of bacteriophage T7. Dimensions of the bacteriophage and its capsids.
Biophysical journal
Projected structure of purple membrane determined to 3.7 A resolution by low temperature electron microscopy.
Journal of molecular biology
Attachment site(s) of retinal in bacteriorhodopsin.
Proceedings of the National Academy of Sciences of the United States of America
Crystalline arrays of membrane-bound acetylcholine receptor.
Proceedings of the National Academy of Sciences of the United States of America
Quantitative analysis of electrophoretograms: a mathematical approach to super-resolution.
Analytical biochemistry
Purification and characterization of a macromolecular weight cofactor for C3b-inactivator, C4bC3bINA-cofactor, of human plasma.
Molecular immunology
Protease effects on the structure of acetylcholine receptor membranes from Torpedo californica.
The Journal of cell biology
Immunospecific identification and three-dimensional structure of a membrane-bound acetylcholine receptor from Torpedo californica.
Journal of molecular biology
Structure determination of asymmetric membrane profiles using an iterative Fourier method.
Biophysical journal
Structural studies of a membrane-bound acetylcholine receptor from Torpedo californica.
Journal of molecular biology
Structure of bovine trypsinogen at 1.9 A resolution.
Biochemistry
Mechanisms of zymogen activation.
Annual review of biophysics and bioengineering
pH dependence of tritium exchange with the C-2 protons of the histidines in bovine trypsin.
Biochemistry
A pulsed diffusion technique for the growth of protein crystals for x-ray diffraction.
Journal of molecular biology
The structural basis for binding of complement by immunoglobulin M.
The Journal of experimental medicine
Silver ion inhibition of serine proteases: crystallographic study of silver-trypsin.
Biochemical and biophysical research communications
A family of protein-cutting proteins.
Scientific American
Structure and specific binding of trypsin: comparison of inhibited derivatives and a model for substrate binding.
Journal of molecular biology
The structure of bovine trypsin: electron density maps of the inhibited enzyme at 5 Angstrom and at 2-7 Angstron resolution.
Journal of molecular biology
The crystal and molecular structure of DIP-inhibited bovine trypsin at2.7Angstrom resolution.
Cold Spring Harbor symposia on quantitative biology