
Professor
Cellular Molecular Pharmacology
+1 415 502-8080
Publications
Programmable microparticles rewire CAR signaling to enable super-physiological expansion of human T cells in vitro.
bioRxiv : the preprint server for biology
Engineered SH3-Derived Sherpabodies Function as a Modular Platform for Targeted T-cell Immunotherapy.
Cancer research
Abstract 6114: Engineering novel synNotch CAR T cells to precisely and effectively target medulloblastoma.
Cancer research
Engineering synthetic suppressor T cells that execute locally targeted immunoprotective programs.
Science (New York, N.Y.)
Programming tissue-sensing T cells that deliver therapies to the brain.
Science (New York, N.Y.)
De novo-designed minibinders expand the synthetic biology sensing repertoire.
bioRxiv : the preprint server for biology
Degron-Based bioPROTACs for Controlling Signaling in CAR T Cells.
ACS synthetic biology
DIPG-15. NOVEL CNS SENSING SYNNOTCH-CAR T CELLS FOR TARGETING DIFFUSE MIDLINE GLIOMA.
Neuro-Oncology
Degron-based bioPROTACs for controlling signaling in CAR T cells.
bioRxiv : the preprint server for biology
The General Characteristics and Design Taxonomy of Chatbots for COVID-19: A Systematic Review.
Journal of medical Internet research
Golden age of immunoengineering.
Immunological reviews
An immune-based tool platform for in vivo cell clearance.
Life science alliance
Instructional materials that control cellular activity through synthetic Notch receptors.
Biomaterials
CAR-T Cell-Mediated B-Cell Depletion in Central Nervous System Autoimmunity.
Neurology(R) neuroimmunology & neuroinflammation
Synthetic cytokine circuits that drive T cells into immune-excluded tumors.
Science (New York, N.Y.)
Decoding CAR T cell phenotype using combinatorial signaling motif libraries and machine learning.
Science (New York, N.Y.)
The emerging era of cell engineering: Harnessing the modularity of cells to program complex biological function.
Science (New York, N.Y.)
Rethinking cancer targeting strategies in the era of smart cell therapeutics.
Nature reviews. Cancer
Deep-learning-based analysis of combinatorial synthetic T-cell receptor libraries.
Biophysical journal
Precise control of immune modulation using DNA scaffold-mediated biomaterial functionalization.
The Journal of Immunology
SynNotch-CAR T cells overcome challenges of specificity, heterogeneity, and persistence in treating glioblastoma.
Science translational medicine
T cells selectively filter oscillatory signals on the minutes timescale.
Proceedings of the National Academy of Sciences of the United States of America
T cell circuits that sense antigen density with an ultrasensitive threshold.
Science (New York, N.Y.)
DNA scaffolds enable efficient and tunable functionalization of biomaterials for immune cell modulation.
Nature nanotechnology
Engineering cytokines and cytokine circuits.
Science (New York, N.Y.)
Precise T cell recognition programs designed by transcriptionally linking multiple receptors.
Science (New York, N.Y.)
Engineering synthetic morphogen systems that can program multicellular patterning.
Science (New York, N.Y.)
What is the Optimal Design-Build-Test Cycle for Clinically Relevant Synthetic CAR T Cell Therapies?
Cell systems
DNA-scaffolded biomaterials enable modular and tunable presentation of proteins to control immune cell therapies.
The Journal of Immunology
Engineering T Cells to Treat Cancer: The Convergence of Immuno-Oncology and Synthetic Biology.
Annual Review of Cancer Biology
IMMU-21. SEQUENTIAL TWO-RECEPTOR PRIMING CAR SYSTEM TO OVERCOME HETEROGENEOUS ANTIGEN EXPRESSION.
Neuro-Oncology
The Design Principles of Biochemical Timers: Circuits that Discriminate between Transient and Sustained Stimulation.
Cell systems
High-throughput multicolor optogenetics in microwell plates.
Nature protocols
Engineering cell-cell communication networks: programming multicellular behaviors.
Current opinion in chemical biology
Abstract A031: Engineering antigen density sensors for T-cell immunotherapy.
Cancer Immunology Research
Cancer mutations and targeted drugs can disrupt dynamic signal encoding by the Ras-Erk pathway.
Science (New York, N.Y.)
Programming self-organizing multicellular structures with synthetic cell-cell signaling.
Science (New York, N.Y.)
Tracing Information Flow from Erk to Target Gene Induction Reveals Mechanisms of Dynamic and Combinatorial Control.
Molecular cell
Synthetic Immunology: Hacking Immune Cells to Expand Their Therapeutic Capabilities.
Annual review of immunology
CRISPR/Cas9-mediated PD-1 disruption enhances anti-tumor efficacy of human chimeric antigen receptor T cells.
Scientific reports
Interrogating cellular perception and decision making with optogenetic tools.
The Journal of cell biology
Engineering Therapeutic T Cells: From Synthetic Biology to Clinical Trials.
Annual review of pathology
Engineering dynamical control of cell fate switching using synthetic phospho-regulons.
Proceedings of the National Academy of Sciences of the United States of America
Engineering T Cells with Customized Therapeutic Response Programs Using Synthetic Notch Receptors.
Cell
Cellular perception and misperception: Internal models for decision-making shaped by evolutionary experience.
BioEssays : news and reviews in molecular, cellular and developmental biology
Modular engineering of cellular signaling proteins and networks.
Current opinion in structural biology
CRISPR/Cas9 for Human Genome Engineering and Disease Research.
Annual review of genomics and human genetics
CRISPR/Cas9-mediated PD-1 disruption enhances anti-tumor efficacy of human chimeric antigen receptor T cells.
The Journal of Immunology
Expanding the CRISPR imaging toolset with Staphylococcus aureus Cas9 for simultaneous imaging of multiple genomic loci.
Nucleic acids research
Beyond editing: repurposing CRISPR-Cas9 for precision genome regulation and interrogation.
Nature reviews. Molecular cell biology
Oscillatory stress stimulation uncovers an Achilles' heel of the yeast MAPK signaling network.
Science (New York, N.Y.)
Remote control of therapeutic T cells through a small molecule-gated chimeric receptor.
Science (New York, N.Y.)
Synthetic biology approaches to engineer T cells.
Current opinion in immunology
Differential genetic interactions of yeast stress response MAPK pathways.
Molecular systems biology
Specific gene repression by CRISPRi system transferred through bacterial conjugation.
ACS synthetic biology
Synthetic control of mammalian-cell motility by engineering chemotaxis to an orthogonal bioinert chemical signal.
Proceedings of the National Academy of Sciences of the United States of America
Exploitation of latent allostery enables the evolution of new modes of MAP kinase regulation (609.5).
The FASEB Journal
Secreting and sensing the same molecule allows cells to achieve versatile social behaviors.
Science (New York, N.Y.)
Using optogenetics to interrogate the dynamic control of signal transmission by the Ras/Erk module.
Cell
Genetic sensor for strong methylating compounds.
ACS synthetic biology
Actin dynamics rapidly reset chemoattractant receptor sensitivity following adaptation in neutrophils.
Philosophical transactions of the Royal Society of London. Series B, Biological sciences
Counting molecules in single organelles with superresolution microscopy allows tracking of the endosome maturation trajectory.
Proceedings of the National Academy of Sciences of the United States of America
Signalling change: signal transduction through the decades.
Nature reviews. Molecular cell biology
Cell-based therapeutics: the next pillar of medicine.
Science translational medicine
Bridging cross-cultural gaps in scientific exchange through innovative team challenge workshops.
Quantitative biology (Beijing, China)
Design principles of regulatory networks: searching for the molecular algorithms of the cell.
Molecular cell
Cell biology 2.0.
Trends in cell biology
Wendell Lim: exploring the path not chosen. Interview by Caitlin Sedwick.
The Journal of cell biology
The first World Cell Race.
Current biology : CB
Conformational control of the Ste5 scaffold protein insulates against MAP kinase misactivation.
Science (New York, N.Y.)
SYNZIP protein interaction toolbox: in vitro and in vivo specifications of heterospecific coiled-coil interaction domains.
ACS synthetic biology
Control of protein signaling using a computationally designed GTPase/GEF orthogonal pair.
Proceedings of the National Academy of Sciences of the United States of America
Recruitment interactions can override catalytic interactions in determining the functional identity of a protein kinase.
Proceedings of the National Academy of Sciences of the United States of America
Scaffold proteins: hubs for controlling the flow of cellular information.
Science (New York, N.Y.)
Regulation of NR4A nuclear receptor expression by oncogenic BRAF in melanoma cells.
Pigment cell & melanoma research
Light control of plasma membrane recruitment using the Phy-PIF system.
Methods in enzymology
The promise of optogenetics in cell biology: interrogating molecular circuits in space and time.
Nature methods
Build life to understand it.
Nature
Designing customized cell signalling circuits.
Nature reviews. Molecular cell biology
Rapid diversification of cell signaling phenotypes by modular domain recombination.
Science (New York, N.Y.)
Defining network topologies that can achieve biochemical adaptation.
The FASEB Journal
The Design Logic of Cell Signaling Systems.
The FASEB Journal
Deciphering protein kinase specificity through large-scale analysis of yeast phosphorylation site motifs.
Science signaling
Rewiring cells: synthetic biology as a tool to interrogate the organizational principles of living systems.
Annual review of biophysics
A reciprocal interdependence between Nck and PI(4,5)P(2) promotes localized N-WASp-mediated actin polymerization in living cells.
Molecular cell
Scaffolds: interaction platforms for cellular signalling circuits.
Trends in cell biology
Positive selection of tyrosine loss in metazoan evolution.
Science (New York, N.Y.)
Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.
PLoS biology
Frederic M Richards 1925-2009.
Nature structural & molecular biology
Evolution of the phospho-tyrosine signaling machinery in premetazoan lineages.
Proceedings of the National Academy of Sciences of the United States of America
Using engineered scaffold interactions to reshape MAP kinase pathway signaling dynamics.
Science (New York, N.Y.)
A microfluidic system for dynamic yeast cell imaging.
BioTechniques
Synthetic biology: lessons from the history of synthetic organic chemistry.
Nature chemical biology
Engineering synthetic signaling proteins with ultrasensitive input/output control.
Nature biotechnology
Engineering modular protein interaction switches by sequence overlap.
Journal of the American Chemical Society
Multiple WASP-interacting protein recognition motifs are required for a functional interaction with N-WASP.
The Journal of biological chemistry
Beta strand peptidomimetics as potent PDZ domain ligands.
Chemistry & biology
Docking interactions in protein kinase and phosphatase networks.
Current opinion in structural biology
The Modular Logic of Cell Signaling Systems.
The FASEB Journal
The Ste5 scaffold allosterically modulates signaling output of the yeast mating pathway.
Science (New York, N.Y.)
Domains, motifs, and scaffolds: the role of modular interactions in the evolution and wiring of cell signaling circuits.
Annual review of biochemistry
The role of docking interactions in mediating signaling input, output, and discrimination in the yeast MAPK network.
Molecular cell
A general model for preferential hetero-oligomerization of LIN-2/7 domains: mechanism underlying directed assembly of supramolecular signaling complexes.
The Journal of biological chemistry
Rewiring cell signaling: the logic and plasticity of eukaryotic protein circuitry.
Current opinion in structural biology
Sho1 and Pbs2 act as coscaffolds linking components in the yeast high osmolarity MAP kinase pathway.
Molecular cell
Reprogramming control of an allosteric signaling switch through modular recombination.
Science (New York, N.Y.)
Complex inheritance of familial hypercholanemia with associated mutations in TJP2 and BAAT.
Nature genetics
The structure and function of proline recognition domains.
Science's STKE : signal transduction knowledge environment
Rewiring MAP kinase pathways using alternative scaffold assembly mechanisms.
Science (New York, N.Y.)
Shared receptors in axon guidance: SAX-3/Robo signals via UNC-34/Enabled and a Netrin-independent UNC-40/DCC function.
Nature neuroscience
Coordinated folding and association of the LIN-2, -7 (L27) domain. An obligate heterodimerization involved in assembly of signaling and cell polarity complexes.
The Journal of biological chemistry
How signaling proteins integrate multiple inputs: a comparison of N-WASP and Cdk2.
Current opinion in cell biology
From folding towards function.
Current opinion in structural biology
The modular logic of signaling proteins: building allosteric switches from simple binding domains.
Current opinion in structural biology
Structure of the SH3-guanylate kinase module from PSD-95 suggests a mechanism for regulated assembly of MAGUK scaffolding proteins.
Molecular cell
Mechanism and role of PDZ domains in signaling complex assembly.
Journal of cell science
The double life of PX domains.
Nature structural biology
An analysis of the interactions between the Sem-5 SH3 domain and its ligands using molecular dynamics, free energy calculations, and sequence analysis.
Journal of the American Chemical Society
Integration of multiple signals through cooperative regulation of the N-WASP-Arp2/3 complex.
Science (New York, N.Y.)
Converging on proline: the mechanism of WW domain peptide recognition.
Nature structural biology
Improving SH3 domain ligand selectivity using a non-natural scaffold.
Chemistry & biology
PSD-95 assembles a ternary complex with the N-methyl-D-aspartic acid receptor and a bivalent neuronal NO synthase PDZ domain.
The Journal of biological chemistry
Unexpected modes of PDZ domain scaffolding revealed by structure of nNOS-syntrophin complex.
Science (New York, N.Y.)
Exploiting the basis of proline recognition by SH3 and WW domains: design of N-substituted inhibitors.
Science (New York, N.Y.)
How Src exercises self-restraint.
Nature structural biology
Reading between the lines: SH3 recognition of an intact protein.
Structure (London, England : 1993)
Structural determinants of peptide-binding orientation and of sequence specificity in SH3 domains.
Nature
Stability and peptide binding affinity of an SH3 domain from the Caenorhabditis elegans signaling protein Sem-5.
Protein science : a publication of the Protein Society
Critical residues in an SH3 domain from Sem-5 suggest a mechanism for proline-rich peptide recognition.
Nature structural biology
The crystal structure of a mutant protein with altered but improved hydrophobic core packing.
Proceedings of the National Academy of Sciences of the United States of America
An analysis of packing in the protein folding problem.
Quarterly reviews of biophysics
The role of internal packing interactions in determining the structure and stability of a protein.
Journal of molecular biology
Analysis of DNA-protein interactions by affinity coelectrophoresis.
Methods in enzymology
Random mutagenesis of protein sequences using oligonucleotide cassettes.
Methods in enzymology
Deciphering the message in protein sequences: tolerance to amino acid substitutions.
Science (New York, N.Y.)