HSP90 PDB, SAXS, and EM Codes

 

PDB code revision reference resolution description download PDB
5FWK   Verba, et al, 2016 3.9Å cryoEM structure of Hsp90-Cdc37-Cdk4 complex 5FWK
5FWL   9.0Å cryoEM structure of Hsp90-Cdc37-Cdk4 complex 5FWL
5FWM   8.0Å cryoEM structure of Hsp90-Cdc37-Cdk4 complex 5FWM
5FWP   7.2Å cryoEM structure of Hsp90-Cdc37-Cdk4 complex 5FWP
    Kollman, et al, 2015   TuSC closed state TuSC-Closed
4IYN   Lavery, et al, 2014 2.31Å Structure of mitochondrial Hsp90 (TRAP1) with ADP-AlF4- 4IYN
4J0B   2.35Å Structure of mitochondrial Hsp90 (TRAP1) with ADP-BeF3 4J0B
4IPE   2.29Å Structure of mitochondrial Hsp90 (TRAP1) with AMPPNP 4IPE
4IVG   1.75Å Crystal structure of mitochondrial Hsp90 (TRAP1) NTD-Middle domain dimer with AMPPNP 4IVG
    Krukenberg, et al, 2009   Grp94 Extended SAXS Model Grp94
      Hsc82 Extended SAXS Model Hsc82
      Hsp90 Extended SAXS Model Hsp90
      HtpG High pH SAXS Model HtpG-Hi
      HtpG Low pH SAXS Model HtpG-Lo
2GQ0   Shiau, et al, 2006 1.9Å Crystal Structure of the Middle Domain of HtpG, the E. coli Hsp90 2GQ0
2IOR   1.65Å Crystal Structure of the N-terminal Domain of HtpG, the Escherichia coli Hsp90, Bound to ADP 2IOR
2IOQ   3.5Å Crystal Structure of Full-length HtpG, Escherichia coli Hsp90 2IOQ
2IOP    3.55Å Crystal Structure of Full-length HtpG, the Escherichia coli Hsp90, Bound to ADP 2IOP
      Structure of the extended dimer of HtpG, the Escherichia coli Hsp90, Bound to ADP ADP-EXT
      Structure of the proposed compact dimer of HtpG, the Escheria coli Hsp90, Bound to ADP ADP-CLOSED
1SF8 19 Feb 2004

Harris, et al, 2004

 2.60Å Crystal structure of the carboxy-terminal domain of htpG, the E. coli Hsp90 1SF8

References

"Atomic Structure of Hsp90:Cdc37:Cdk4 Reveals that Hsp90 Traps and Stabilizes an Unfolded Kinase, Verba, K.A., Wang, R.Y.R., Arakawa, A., Liu, Y., Yokoyama, S., Agard, D.A. , (2016) Science 352: 1542. (html, pdf)

"Hsp70 Forms Antiparallel Dimers Stabilized by Post-translational Modifications to Position Clients for Transfer to Hsp90," Morgner, Nina; Schmidt, Carla; Beilsten-Edmands, Victoria; Ebong, Ima-obong; Patel, Nisha A.; Clerico, Eugenia M; Kirschke, Elaine; Daturpalli, Soumya; Jackson, Sophie E; Agard, David; Robinson, Carol V., Cell Reports (2015), 11(5), 759-69. (html, pdf)

"Structural Asymmetry in the Closed State of Mitochondrial Hsp90 (TRAP1) Supports a Two-Step ATP Hydrolysis Mechanism," Laura A. Lavery, James R. Partridge, Theresa A. Ramelot, Daniel Elnatan, Michael A. Kennedy, David A. Agard, Molecular Cell, Volume 53, Issue 2, 23 January 2014, Pages 330-343 (html, pdf)

Krukenberg KA, Southworth DR, Street TO, Agard DA, "pH-Dependent Conformational Changes in Bacterial Hsp90 Reveal a Grp94-Like Conformation at pH 6 That Is Highly Active in Suppression of Citrate Synthase Aggregation," J Mol Biol. 2009 May 7. (html, pdf)

Shiau AK, Harris SF, Southworth DR, Agard DA, "Structural Analysis of E. coli hsp90 Reveals Dramatic Nucleotide-Dependent Conformational Rearrangements," Cell. 2006 Oct 20;127(2):329-340 (html or pdf).

Seth F. Harris, Andrew K. Shiau and David A. Agard, "The Crystal Structure of the Carboxy-Terminal Dimerization Domain of htpG, the Escherichia coli Hsp90, Reveals a Potential Substrate Binding Site," Structure, (2004) 12(6):1087-1097 (pdf).