Aashish Manglik, MD, PhD

Assistant Professor
Department of Pharmaceutical Chemistry
+1 415 502-3744

Aashish was an undergraduate at Washington University in St. Louis, where he worked in the lab of Jeff McKinney on Salmonella-host interactions. He moved to California in 2008 to join the Stanford Medical Scientist Training Program. There, he worked with Brian Kobilka as a graduate student to elucidate different aspects of GPCR function, resulting in a number of important contributions to our current understanding of opioid and adrenergic receptors. After finishing his medical training in May 2016, Aashish began his independent research career as the first Stanford Distinguished Fellow at Stanford University School of Medicine within the Department of Molecular and Cellular Physiology. He subsequently began as an assistant professor at UCSF in fall of 2017.

Publications: 

Development of an antibody fragment that stabilizes GPCR/G-protein complexes.

Nature communications

Maeda S, Koehl A, Matile H, Hu H, Hilger D, Schertler GFX, Manglik A, Skiniotis G, Dawson RJP, Kobilka BK

Measuring ligand efficacy at the mu-opioid receptor using a conformational biosensor.

eLife

Livingston KE, Mahoney JP, Manglik A, Sunahara R, Traynor JR

Structure of the µ-opioid receptor-Gi protein complex.

Nature

Koehl A, Hu H, Maeda S, Zhang Y, Qu Q, Paggi JM, Latorraca NR, Hilger D, Dawson R, Matile H, Schertler GFX, Granier S, Weis WI, Dror RO, Manglik A, Skiniotis G, Kobilka BK

A Genetically Encoded Biosensor Reveals Location Bias of Opioid Drug Action.

Neuron

Stoeber M, Jullié D, Lobingier BT, Laeremans T, Steyaert J, Schiller PW, Manglik A, von Zastrow M

Sortase ligation enables homogeneous GPCR phosphorylation to reveal diversity in ß-arrestin coupling.

Proceedings of the National Academy of Sciences of the United States of America

Staus DP, Wingler LM, Choi M, Pani B, Manglik A, Kruse AC, Lefkowitz RJ

Yeast surface display platform for rapid discovery of conformationally selective nanobodies.

Nature structural & molecular biology

McMahon C, Baier AS, Pascolutti R, Wegrecki M, Zheng S, Ong JX, Erlandson SC, Hilger D, Rasmussen SGF, Ring AM, Manglik A, Kruse AC

Nanobodies to Study G Protein-Coupled Receptor Structure and Function.

Annual review of pharmacology and toxicology

Manglik A, Kobilka BK, Steyaert J

Structure-based discovery of opioid analgesics with reduced side effects.

Nature

Manglik A, Lin H, Aryal DK, McCorvy JD, Dengler D, Corder G, Levit A, Kling RC, Bernat V, Hübner H, Huang XP, Sassano MF, Giguère PM, Löber S

Allosteric nanobodies reveal the dynamic range and diverse mechanisms of G-protein-coupled receptor activation.

Nature

Staus DP, Strachan RT, Manglik A, Pani B, Kahsai AW, Kim TH, Wingler LM, Ahn S, Chatterjee A, Masoudi A, Kruse AC, Pardon E, Steyaert J, Weis WI, Prosser RS, Kobilka BK, Costa T, Lefkowitz RJ

Allosteric coupling from G protein to the agonist-binding pocket in GPCRs.

Nature

DeVree BT, Mahoney JP, Vélez-Ruiz GA, Rasmussen SG, Kuszak AJ, Edwald E, Fung JJ, Manglik A, Masureel M, Du Y, Matt RA, Pardon E, Steyaert J, Kobilka BK, Sunahara RK

Crystal structure of the human s1 receptor.

Nature

Schmidt HR, Zheng S, Gurpinar E, Koehl A, Manglik A, Kruse AC

High-density grids for efficient data collection from multiple crystals.

Acta crystallographica. Section D, Structural biology

Baxter EL, Aguila L, Alonso-Mori R, Barnes CO, Bonagura CA, Brehmer W, Brunger AT, Calero G, Caradoc-Davies TT, Chatterjee R, Degrado WF, Fraser JS, Ibrahim M, Kern J, Kobilka BK, Kruse AC, Larsson KM, Lemke HT, Lyubimov AY, Manglik A, McPhillips SE, Norgren E, Pang SS, Soltis SM, Song J, Thomaston J, Tsai Y, Weis WI, Woldeyes RA, Yachandra V, Yano J, Zouni A, Cohen AE

Engineering high-affinity PD-1 variants for optimized immunotherapy and immuno-PET imaging.

Proceedings of the National Academy of Sciences of the United States of America

Maute RL, Gordon SR, Mayer AT, McCracken MN, Natarajan A, Ring NG, Kimura R, Tsai JM, Manglik A, Kruse AC, Gambhir SS, Weissman IL, Ring AM

Propagation of conformational changes during µ-opioid receptor activation.

Nature

Sounier R, Mas C, Steyaert J, Laeremans T, Manglik A, Huang W, Kobilka BK, Déméné H, Granier S

Structural insights into µ-opioid receptor activation.

Nature

Huang W, Manglik A, Venkatakrishnan AJ, Laeremans T, Feinberg EN, Sanborn AL, Kato HE, Livingston KE, Thorsen TS, Kling RC, Granier S, Gmeiner P, Husbands SM, Traynor JR, Weis WI, Steyaert J, Dror RO, Kobilka BK

SIGNAL TRANSDUCTION. Structural basis for nucleotide exchange in heterotrimeric G proteins.

Science (New York, N.Y.)

Dror RO, Mildorf TJ, Hilger D, Manglik A, Borhani DW, Arlow DH, Philippsen A, Villanueva N, Yang Z, Lerch MT, Hubbell WL, Kobilka BK, Sunahara RK, Shaw DE

Structural Insights into the Dynamic Process of ß2-Adrenergic Receptor Signaling.

Cell

Manglik A, Kim TH, Masureel M, Altenbach C, Yang Z, Hilger D, Lerch MT, Kobilka TS, Thian FS, Hubbell WL, Prosser RS, Kobilka BK

G Protein-coupled Receptor Kinases of the GRK4 Protein Subfamily Phosphorylate Inactive G Protein-coupled Receptors (GPCRs).

The Journal of biological chemistry

Li L, Homan KT, Vishnivetskiy SA, Manglik A, Tesmer JJ, Gurevich VV, Gurevich EV

Goniometer-based femtosecond crystallography with X-ray free electron lasers.

Proceedings of the National Academy of Sciences of the United States of America

Cohen AE, Soltis SM, González A, Aguila L, Alonso-Mori R, Barnes CO, Baxter EL, Brehmer W, Brewster AS, Brunger AT, Calero G, Chang JF, Chollet M, Ehrensberger P, Eriksson TL, Feng Y, Hattne J, Hedman B, Hollenbeck M, Holton JM, Keable S, Kobilka BK, Kovaleva EG, Kruse AC, Lemke HT, Lin G, Lyubimov AY, Manglik A, Mathews II, McPhillips SE, Nelson S, Peters JW, Sauter NK, Smith CA, Song J, Stevenson HP, Tsai Y, Uervirojnangkoorn M, Vinetsky V, Wakatsuki S, Weis WI, Zadvornyy OA, Zeldin OB, Zhu D, Hodgson KO

Covalent agonists for studying G protein-coupled receptor activation.

Proceedings of the National Academy of Sciences of the United States of America

Weichert D, Kruse AC, Manglik A, Hiller C, Zhang C, Hübner H, Kobilka BK, Gmeiner P

Activation and allosteric modulation of a muscarinic acetylcholine receptor.

Nature

Kruse AC, Ring AM, Manglik A, Hu J, Hu K, Eitel K, Hübner H, Pardon E, Valant C, Sexton PM, Christopoulos A, Felder CC, Gmeiner P, Steyaert J, Weis WI, Garcia KC, Wess J, Kobilka BK

Applications of molecular replacement to G protein-coupled receptors.

Acta crystallographica. Section D, Biological crystallography

Kruse AC, Manglik A, Kobilka BK, Weis WI

Adrenaline-activated structure of ß2-adrenoceptor stabilized by an engineered nanobody.

Nature

Ring AM, Manglik A, Kruse AC, Enos MD, Weis WI, Garcia KC, Kobilka BK

The role of ligands on the equilibria between functional states of a G protein-coupled receptor.

Journal of the American Chemical Society

Kim TH, Chung KY, Manglik A, Hansen AL, Dror RO, Mildorf TJ, Shaw DE, Kobilka BK, Prosser RS

Structure of active ß-arrestin-1 bound to a G-protein-coupled receptor phosphopeptide.

Nature

Shukla AK, Manglik A, Kruse AC, Xiao K, Reis RI, Tseng WC, Staus DP, Hilger D, Uysal S, Huang LY, Paduch M, Tripathi-Shukla P, Koide A, Koide S, Weis WI, Kossiakoff AA, Kobilka BK, Lefkowitz RJ

The dynamic process of ß(2)-adrenergic receptor activation.

Cell

Nygaard R, Zou Y, Dror RO, Mildorf TJ, Arlow DH, Manglik A, Pan AC, Liu CW, Fung JJ, Bokoch MP, Thian FS, Kobilka TS, Shaw DE, Mueller L, Prosser RS, Kobilka BK

Role of detergents in conformational exchange of a G protein-coupled receptor.

The Journal of biological chemistry

Chung KY, Kim TH, Manglik A, Alvares R, Kobilka BK, Prosser RS

A new class of amphiphiles bearing rigid hydrophobic groups for solubilization and stabilization of membrane proteins.

Chemistry (Weinheim an der Bergstrasse, Germany)

Chae PS, Rasmussen SG, Rana RR, Gotfryd K, Kruse AC, Manglik A, Cho KH, Nurva S, Gether U, Guan L, Loland CJ, Byrne B, Kobilka BK, Gellman SH

Structure of the d-opioid receptor bound to naltrindole.

Nature

Granier S, Manglik A, Kruse AC, Kobilka TS, Thian FS, Weis WI, Kobilka BK

Crystal structure of the µ-opioid receptor bound to a morphinan antagonist.

Nature

Manglik A, Kruse AC, Kobilka TS, Thian FS, Mathiesen JM, Sunahara RK, Pardo L, Weis WI, Kobilka BK, Granier S

Stably integrated luxCDABE for assessment of Salmonella invasion kinetics.

Molecular imaging

Flentie KN, Qi M, Gammon ST, Razia Y, Lui F, Marpegan L, Manglik A, Piwnica-Worms D, McKinney JS