Adam Frost, PhD, MD

ASSOC ADJ PROF-FY
Biochemistry and BIophysics
[email protected]
+1 415 514-4825
Publications: 

Directed evolution of the rRNA methylating enzyme Cfr reveals molecular basis of antibiotic resistance.

eLife

Tsai K, Stojkovic V, Noda-Garcia L, Young ID, Myasnikov AG, Kleinman J, Palla A, Floor SN, Frost A, Fraser JS, Tawfik DS, Fujimori DG

Viral evasion of the integrated stress response through antagonism of eIF2-P binding to eIF2B.

Nature communications

Schoof M, Wang L, Cogan JZ, Lawrence RE, Boone M, Wuerth JD, Frost A, Walter P

Ribosome-associated quality control and CAT tailing.

Critical reviews in biochemistry and molecular biology

Howard CJ, Frost A

Activation of the Exocyst Tethering Complex for SNARE Complex Regulation and Membrane Fusion.

The FASEB Journal

Mary Munson, Dante Lepore, Michael Feyder, Guendalina Rossi, Alexander Czuchra, Leonora Martínez-Núñez, Lillian Kenner, Adam Frost, Patrick Brennwald

eIF2B Conformation and Assembly State Regulate the Integrated Stress Response.

The FASEB Journal

Michael Schoof, Morgane Boone, Lan Wang, Rosalie Lawrence, Adam Frost, Peter Walter

Practical considerations for using K3 cameras in CDS mode for high-resolution and high-throughput single particle cryo-EM.

Journal of structural biology

Sun M, Azumaya C, Tse E, Frost A, Southworth D, Verba KA, Cheng Y, Agard DA

Structural and mechanistic basis of the EMC-dependent biogenesis of distinct transmembrane clients.

eLife

Miller-Vedam LE, Bräuning B, Popova KD, Schirle Oakdale NT, Bonnar JL, Prabu JR, Boydston EA, Sevillano N, Shurtleff MJ, Stroud RM, Craik CS, Schulman BA, Frost A, Weissman JS

Practical Considerations of Using the K3 Camera in CDS Mode for High-resolution and High-throughput Single Particle Cryo-EM.

Microscopy and Microanalysis

Ming Sun, Caleigh Azumaya, Eric Tse, Daniel Southworth, Kliment Verba, Adam Frost, Yifan Cheng, David Agard

GIGYF2 and 4EHP Inhibit Translation Initiation of Defective Messenger RNAs to Assist Ribosome-Associated Quality Control.

Molecular cell

Hickey KL, Dickson K, Cogan JZ, Replogle JM, Schoof M, D'Orazio KN, Sinha NK, Hussmann JA, Jost M, Frost A, Green R, Weissman JS, Kostova KK

LEM2 phase separation promotes ESCRT-mediated nuclear envelope reformation.

Nature

von Appen A, LaJoie D, Johnson IE, Trnka MJ, Pick SM, Burlingame AL, Ullman KS, Frost A

Membrane constriction and thinning by sequential ESCRT-III polymerization.

Nature structural & molecular biology

Nguyen HC, Talledge N, McCullough J, Sharma A, Moss FR, Iwasa JH, Vershinin MD, Sundquist WI, Frost A

Activation of the Exocyst Tethering Complex for SNARE Complex Regulation and Membrane Fusion.

The FASEB Journal

Mary Munson, Dante Lepore, Michael Feyder, Guendalina Rossi, Alexander B Czuchra, Lillian Kenner, Leonora Martínez-Núñez, Adam Frost, Patrick Brennwald

Assessment of the nucleotide modifications in the high-resolution cryo-electron microscopy structure of the Escherichia coli 50S subunit.

Nucleic acids research

Stojkovic V, Myasnikov AG, Young ID, Frost A, Fraser JS, Fujimori DG

Exocyst structural changes associated with activation of tethering downstream of Rho/Cdc42 GTPases.

The Journal of cell biology

Rossi G, Lepore D, Kenner L, Czuchra AB, Plooster M, Frost A, Munson M, Brennwald P

Exocyst Tethering Complex Regulation of SNARE Proteins and Membrane Fusion.

Biophysical journal

Mary Munson, Dante Lepore, Michael Feyder, Guendalina Rossi, Alexander B. Czuchra, Lillian Kenner, Leonora Martinez-Nunez, Jacqueline M. Forson, Adam Frost, Patrick Brennwald

Membrane Constriction and Thinning by Sequential ESCRT-III Polymerization.

Biophysical journal

Henry C. Nguyen, Nathaniel Talledge, John McCullough, Abhimanyu Sharma, Frank R. Moss, Janet Iwasa, Michael Vershinin, Wesley I. Sundquist, Adam Frost

eIF2B-catalyzed nucleotide exchange and phosphoregulation by the integrated stress response.

Science (New York, N.Y.)

Kenner LR, Anand AA, Nguyen HC, Myasnikov AG, Klose CJ, McGeever LA, Tsai JC, Miller-Vedam LE, Walter P, Frost A

Functional role of PGAM5 multimeric assemblies and their polymerization into filaments.

Nature communications

Ruiz K, Thaker TM, Agnew C, Miller-Vedam L, Trenker R, Herrera C, Ingaramo M, Toso D, Frost A, Jura N

FDM 3D Printing of High-Pressure, Heat-Resistant, Transparent Microfluidic Devices.

Analytical chemistry

Romanov V, Samuel R, Chaharlang M, Jafek AR, Frost A, Gale BK

Structures, Functions, and Dynamics of ESCRT-III/Vps4 Membrane Remodeling and Fission Complexes.

Annual review of cell and developmental biology

McCullough J, Frost A, Sundquist WI

Structural basis of mitochondrial receptor binding and constriction by DRP1.

Nature

Kalia R, Wang RY, Yusuf A, Thomas PV, Agard DA, Shaw JM, Frost A

Vms1p is a release factor for the ribosome-associated quality control complex.

Nature communications

Zurita Rendón O, Fredrickson EK, Howard CJ, Van Vranken J, Fogarty S, Tolley ND, Kalia R, Osuna BA, Shen PS, Hill CP, Frost A, Rutter J

The ER membrane protein complex interacts cotranslationally to enable biogenesis of multipass membrane proteins.

eLife

Shurtleff MJ, Itzhak DN, Hussmann JA, Schirle Oakdale NT, Costa EA, Jonikas M, Weibezahn J, Popova KD, Jan CH, Sinitcyn P, Vembar SS, Hernandez H, Cox J, Burlingame AL, Brodsky J, Frost A, Borner GH, Weissman JS

Structure of the nucleotide exchange factor eIF2B reveals mechanism of memory-enhancing molecule.

Science (New York, N.Y.)

Tsai JC, Miller-Vedam LE, Anand AA, Jaishankar P, Nguyen HC, Renslo AR, Frost A, Walter P

Structural inhibition of dynamin-mediated membrane fission by endophilin.

eLife

Hohendahl A, Talledge N, Galli V, Shen PS, Humbert F, De Camilli P, Frost A, Roux A

CAT-tailing as a fail-safe mechanism for efficient degradation of stalled nascent polypeptides.

Science (New York, N.Y.)

Kostova KK, Hickey KL, Osuna BA, Hussmann JA, Frost A, Weinberg DE, Weissman JS

LEM2 recruits CHMP7 for ESCRT-mediated nuclear envelope closure in fission yeast and human cells.

Proceedings of the National Academy of Sciences of the United States of America

Gu M, LaJoie D, Chen OS, von Appen A, Ladinsky MS, Redd MJ, Nikolova L, Bjorkman PJ, Sundquist WI, Ullman KS, Frost A

Membrane fission by dynamin: what we know and what we need to know.

The EMBO journal

Antonny B, Burd C, De Camilli P, Chen E, Daumke O, Faelber K, Ford M, Frolov VA, Frost A, Hinshaw JE, Kirchhausen T, Kozlov MM, Lenz M, Low HH, McMahon H, Merrifield C, Pollard TD, Robinson PJ, Roux A, Schmid S

A Golgi rhomboid protease Rbd2 recruits Cdc48 to cleave yeast SREBP.

The EMBO journal

Hwang J, Ribbens D, Raychaudhuri S, Cairns L, Gu H, Frost A, Urban S, Espenshade PJ

Subunit connectivity, assembly determinants and architecture of the yeast exocyst complex.

Nature structural & molecular biology

Heider MR, Gu M, Duffy CM, Mirza AM, Marcotte LL, Walls AC, Farrall N, Hakhverdyan Z, Field MC, Rout MP, Frost A, Munson M

Structure and membrane remodeling activity of ESCRT-III helical polymers.

Science (New York, N.Y.)

McCullough J, Clippinger AK, Talledge N, Skowyra ML, Saunders MG, Naismith TV, Colf LA, Afonine P, Arthur C, Sundquist WI, Hanson PI, Frost A

Structural and functional studies of membrane remodeling machines.

Methods in cell biology

Kalia R, Talledge N, Frost A

Molecular Mechanism of ESCRT-III Filament Formation.

The FASEB Journal

John McCullough, Leremy Colf, Marissa Saunders, Matthew Lalonde, Gaelle Mercenne, Wesley Sundquist, Adam Frost

Protein synthesis. Rqc2p and 60S ribosomal subunits mediate mRNA-independent elongation of nascent chains.

Science (New York, N.Y.)

Shen PS, Park J, Qin Y, Li X, Parsawar K, Larson MH, Cox J, Cheng Y, Lambowitz AM, Weissman JS, Brandman O, Frost A

Structural basis of membrane deformation by the ESCRT-III family of proteins (107.4).

The FASEB Journal

Adam Frost, Wes Sundquist, John McCullough, Marissa Saunders

Interchangeable adaptors regulate mitochondrial dynamin assembly for membrane scission.

Proceedings of the National Academy of Sciences of the United States of America

Koirala S, Guo Q, Kalia R, Bui HT, Eckert DM, Frost A, Shaw JM

A ribosome-bound quality control complex triggers degradation of nascent peptides and signals translation stress.

Cell

Brandman O, Stewart-Ornstein J, Wong D, Larson A, Williams CC, Li GW, Zhou S, King D, Shen PS, Weibezahn J, Dunn JG, Rouskin S, Inada T, Frost A, Weissman JS

Functional repurposing revealed by comparing S. pombe and S. cerevisiae genetic interactions.

Cell

Frost A, Elgort MG, Brandman O, Ives C, Collins SR, Miller-Vedam L, Weibezahn J, Hein MY, Poser I, Mann M, Hyman AA, Weissman JS

Endosis and exosis: new names for fusion and budding.

The Journal of membrane biology

Busath DD, Woodbury DJ, Frost A

Structural basis of membrane bending by the N-BAR protein endophilin.

Cell

Mim C, Cui H, Gawronski-Salerno JA, Frost A, Lyman E, Voth GA, Unger VM

The F-BAR domain of srGAP2 induces membrane protrusions required for neuronal migration and morphogenesis.

Cell

Guerrier S, Coutinho-Budd J, Sassa T, Gresset A, Jordan NV, Chen K, Jin WL, Frost A, Polleux F

Mechanistic Insights into Membrane Remodeling Through BAR-Domain Proteins.

The FASEB Journal

Adam Frost, Carsten Mim, Rushika Perera, Krasimir Spasov, Edward Egelman, Pietro De Camilli, Vinzenz M Unger

Structural basis of membrane invagination by F-BAR domains.

Cell

Frost A, Perera R, Roux A, Spasov K, Destaing O, Egelman EH, De Camilli P, Unger VM

F-BAR proteins join the BAR family fold.

Structure (London, England : 1993)

Frost A, De Camilli P, Unger VM

Noncontact dipole effects on channel permeation. VI. 5F- and 6F-Trp gramicidin channel currents.

Biophysical journal

Cole CD, Frost AS, Thompson N, Cotten M, Cross TA, Busath DD

The docking protein FRS2alpha controls a MAP kinase-mediated negative feedback mechanism for signaling by FGF receptors.

Molecular cell

Lax I, Wong A, Lamothe B, Lee A, Frost A, Hawes J, Schlessinger J