Christopher Waddling, PhD

University of California, San Francisco
Department of Biochemistry & Biophysics
600 16th St., S126C
San Francisco CA
94143-2140
(415) 476-8288 (Ph)

X-Ray Facility Manager

UCSF X-ray facility manager is a wide-ranging and dynamic position that includes maintenance of our Rigaku RU-200 X-ray machine and our Anton Paar SAXS system; x-ray safety training; and coordination, maintenance, and operation of our facility. For the past four years, it has included teaching the laboratory section of UCSF's Biophysics219 crystallography course, and this past year, teaching the crystallography component of BI204.

Research

Research projects in the Agard lab have included work on the refinement of a sub-angstrom structure of a mutant of alpha-lytic protease complexed with a BoroAla transition state mimic. Most recently, it has involved solving the crystal structure of a phage tubulin known as PhuZ.

Education and Training

In 2000 I completed a two-year post doctorate at the Wadsworth Center with Dr. Patrick Van Roey where I was involved in the determination of several crystal structures including a high-resolution structure of the peptide-N-acetyl glycoamidase F mutant in complexation with its glycopeptide substrate, the endoglucosidase Endo F3, and complexes with its products; a deletion mutant of human thymadylate synthase complexed with dUMP and the antifolate drug Tomudex; and a novel intertwined structure of the DNA-binding domain of intron endonuclease I-TevI with its substrate.

In 1998 I earned a PhD in Inorganic Chemistry at the University of Hawaii in Honolulu with Dr. Roger Cramer, specializing in the synthesis, kinetics, and structural studies via crystallography of inclusion complexes of a cyclic pyrimidiniophane degradation product of Thiamin.

In 1993 I earned an MSc in Inorganic Chemistry from Queen's University in Kingston, Ontario, Canada, with Dr. Donal Macartney, specializing in reaction mechanisms of inclusion complex formation using alpha-cyclodextrin.

In 1991 I earned a BSc in Chemistry and Physics from the University of Toronto in Toronto, Ontario, where I studied novel phosphonitrile main-group inorganic polymers with Dr. Ian Manners.

Other Interests

Current member of the UCSF University Community Partnerships Councilproject leader of the Palou Community Garden; board member of the Portola Neighborhood Association; maintain and edit D10Watch, a community-based blog that focuses on news and events happening in the Southeast neighborhoods of San Francisco; district representative and Vice Chair of the San Francisco County Transportation Authority Citizens Advisory Committee; founder of Bike Bayview. Past member of the UCSF Chancellor's Advisory Committee on GLBT issues (awarded the 2011 Chancellor's Award for GLBT Leadership for my contributions to the GLBT community at UCSF); former Binational Couples Liaison for Marriage Equality USA.

Publications

"Cytochrome P450 125A4, the Third Cholesterol C-26 Hydroxylase from Myobacterium smagmatis, " Daniel J. Frank, Christopher A. Waddling, Maggie La, and Paul R. Ortiz de Montellano, Biochemistry (2015), 54(46), 6909-16 (html or pdf)

"Broad-Spectrum Allosteric Inhibition of Herpesvirus Proteases, " Jonathan E. Gable, Gregory M. Lee, Priyadarshini Jaishankar, Brian R. Hearn, Christopher A. Waddling, Adam R. Renslo, and Charles S. Craik, Biochemistry (2014), 53(28), 4648-60 (html or pdf)

"A phage tubulin assembles dynamic filaments by a novel mechanism to center viral DNA within the host cell," J. Kraemer, M.L. Erb, C.A. Waddling, E.A. Montabana, E.A. Zehr, H. Wang, K. Nguyen, D.S.L. Pham, D. Agard, and J. Pogliano, Cell, 2012, 149(7), 1488-99 (html or pdf).

"Crystal structures of the dinitrate-bis-(acetylenedicarboxylate) and acetylenedicarboxylate pseudorotaxane complexes of [24-pyrimidinium crown 6]," C.A. Waddling and R.E. Cramer, J. Incl. Phen. and Macro. Chem., 2008, 60(1-2), 1-8 (pdf or htmlabstract online).

"Intertwined Structure of the DNA-Binding Domain of Intron Endonuclease I-TevI with its Substrate: Functional and Evolutionary Insights", P. VanRoey, C.A. Waddling, K.M. Fox, M. Belfort,  and V. Derbyshire, EMBO J., 2001, 20(14), 3631-7. (pdf or html, PDB Code 1I3Jarticles citing this article)

"Crystal Structure of a Deletion Mutant of Human Thymidylate Synthase D(7-29) and its Ternary Complex with Tomudex and dUMP," R. Almog, C.A. Waddling, F. Maley, G. Maley, and P. VanRoeyProtein Science, 2001, 10(5), 988-996. (pdf or html, PDB Code 1HZW and 1I00articles citing this article)

"Structural Basis for the Substrate Specificity of Endo-b-N-acetylglucosamine F3," C.A. Waddling, T.H. Plummer, A.L. Tarentino, and P. VanRoeyBiochemistry, 2000, 39(27), 7878-85. (pdf or html, PDB Code 1EOK and 1EOMarticles citing this article)

"Structurally Novel Bioconversion Products of the Marine Natural Product Sarcophine Effectively Inhibit JB6 Cell Transformation," K.A. El Sayed, M.T. Hamann, C.A. Waddling, C. Jensen, S.K. Lee, C. Andersson Dunstan, and J.M. Pezzuto, J. Org. Chem., 1998, 63(21), 7449-55. (pdf or htmlarticles citing this article)

"Crystal Structures of the Nitrate Salt of 24-Pyrimidinium Crown 6 {5, 12, 19, 26, 33, 40 - hexamino - 3, 10, 17, 24, 31, 38 - hexamethyl [1·6]-(1, 5)pyrimidinionphane and its Degradation Product in Water-Methanol Mixtures," R.E. Cramer, C.A. Waddling, C.H. Fujimoto, D.W. Smith, and K.E. Kim, J. Chem. Soc., Dalton Trans., 1997, 1675-83. (pdfarticles citing this article)

"Polymers with Sulfur(VI)-Nitrogen-Phosphorus Backbones:  Synthesis, Characterization, and Properties of Atactic Poly[(amino)thionylphosphazenes]," Y. Ni, P. Park, M. Liang, J. Massey, C. Waddling, and I. MannersMacromolec., 1996, 29(10), 3401-8. (pdf or htmlarticlesciting this article)

"Kinetic and Spectroscopic Studies of Symmetrical and Asymmetrical Pentascyano(N-heterocycle)ferrate(II) Rotaxanes of a-CD," D.H. Macartney and C.A. Waddling, Inorg. Chem., 1994, 33(25), 5912-19. (abstract or pdf and articles citing this article)

"Catalytic Elimination of Organics over Noble Metal Catalysts Deposited on Metal Structures of Low Mass," S. Suppiah, C. Waddling, and K.J. Kutchkoskie, Stud. Surf. Sci. Catal., 1992, 73 (Prog. Catal.), 187-95. (abstract)

"Ring-opening polymerization as a route to new inorganic macromolecules, "M. Liang, C. Waddling, C. Honeyman, D. Foucher, I. Manners, Phosphorus, Sulfur and Silicon and the Related Elements 1992, 64(1-4), 113-20. (abstractPatent citing this article)

"Poly(thionylphosphazenes); a new class of inorganic polymers with skeletal phosphorus, nitrogen, and sulfur(VI) atoms," M. Liang, C. Waddling, and I. MannersPolymer Preprints, 1991, 32(3), 487-8. (articles citing this article)