Kliment Verba

Department of Biochemistry & Biophysics
University of California, San Francisco
600 16th St, S414
San Francisco CA
94143-2240
(415) 476-5143 (Ph)

Research

Science: I am trying to elucidate Hsp90 interactions with one of its most predominant protein client classes, kinases. Even though for decades Hsp90 interactions have been functionally shown, reconstituting them in vitro is still a major challenge. Using Sf9 expression with our collaborators, we were able to generate a stable Hsp90/Cdc37/kinase complex. Currently, I am working on getting a high resolution cryo EM reconstruction of the Hsp90/Cdc37/Cdk4 complex utilizing the new Direct Detect K2 Summit camera. Such reconstruction will describe what state the proteins are in while interacting and maybe what Hsp90 does to the kinase while bound. Also, I have been working on setting up an E.coli expression system to be able to manipulate the proteins for biochemistry and structural studies using techniques other than EM, like X-ray crystallography and NMR.

Hobbies: in times when I am not observing the alien green glow from the microscope, I like to make stuff. Currently, building a car for rally racing here in California takes up most of my free time. When not working with metal, I like making things out of other materials, like using teat to make kombucha. And when not making stuff I enjoy running or going to Tahoe for crazy snowboarding with my lab mates.

 

Publications:

Cross-Monomer Substrate Contacts Reposition the Hsp90 N-Terminal Domain and Prime the Chaperone Activity. Street TO, Lavery LA, Verba KA, Lee CT, Mayer MP, Agard DA. J Mol Biol. 2012 Oct 31. (pdf)

Atomic structure of Hsp90-Cdc37-Cdk4 reveals that Hps90 traps and stabilizes an unfolded kinase, Verba, Kliment A.; Wang, Ray Yu-Ruei; Arakawa, Akihiko; Liu, Yanxin; Shirouzu, Mikako; Yokoyama, Shigeyuki; Agard, David A., Science. (2016) 352(6293), 1542-7.(htmlpdf)