Michael Braunfeld

Department of Biochemistry & Biophysics
University of California, San Francisco
600 16th St., Room S101
San Francisco CA 94143-2140
(415) 476-4441 (Ph)
(415) 514-4142 (Fax)

Publications:

"Electron counting and beam-induced motion correction enable near-atomic-resolution single-particle cryo-EM," Li, Xueming; Mooney, Paul; Zheng, Shawn; Booth, Christopher R.; Braunfeld, Michael B.; Gubbens, Sander; Agard, David A.; Cheng, Yifan, Nature Methods (2013), 10(6), 584-590. (html, pdf)

"A distributed multi-GPU system for high speed electron microscopic tomographic reconstruction," Zheng, Shawn Q.; Branlund, Eric; Kesthelyi, Bettina; Braunfeld, Michael B.; Cheng, Yifan; Sedat, John W.; Agard, David A., Ultramicroscopy (2011), 111(8), 1137-1143. (pdf)

Zheng SQ, Matsuda A, Braunfeld MB, Sedat JW, Agard DA, "Dual-axis target mapping and automated sequential acquisition of dual-axis EM tomographic data," J Struct Biol. 2009 Jun 21. (html, pdf)

Zheng SQ, Kollman JM, Braunfeld MB, Sedat JW, Agard DA, "Automated acquisition of electron microscopic random conical tilt sets," J Struct Biol. 2007 Jan;157(1):148-55.161.(html or pdf).

Peter König, Michael B. Braunfeld, John W. Sedat and David A. Agard, "The three-dimensional structure of in vitro reconstituted Xenopus laevis chromosomes by EM tomography, " Chromosoma 2007 Mar (pdf).

Wei B, Lam PG, Braunfeld MB, Agard DA, Genzer J, Spontak RJ., "Tunable instability mechanisms of polymer thin films by molecular self-assembly," Langmuir. 2006 Oct 10;22(21):8642-5 (html or pdf).

Yonekura K, Braunfeld MB, Maki-Yonekura S, and Agard DA"Electron energy filtering significantly improves amplitude contrast of frozen-hydrated protein at 300kV," J Struct Biol. 2006 Aug 15 (html or pdf).

Zheng SQ, Keszthelyi B, Branlund E, Lyle JM, Braunfeld MB, Sedat JW, and Agard DA,"UCSF tomography: An integrated software suite for real-time electron microscopic tomographic data collection, alignment, and reconstruction," J Struct Biol. 2006 Jun 23 (html or pdf).

Koenig P, Braunfeld M, Agard DA., "Use of surface affinity enrichment and cryo-embedding to prepare in vitro reconstituted mitotic chromosomes for EM tomography." Ultramicroscopy. 2005 Jul;103(4):261-74. (html or pdf)

Satoru Uzawa, Fei Li, Ye Jin, Kent L. McDonald, Michael B. Braunfeld , David A. Agard , and W. Zacheus Cande, "Spindle Pole Body Duplication in Fission Yeast Occurs at the G1/S Boundary but Maturation Is Blocked until Exit from S by an Event Downstream of Cdc10+." Mol. Biol. Cell, 15(12), 5219-5230. (html or pdf)

Zheng, Q.S., Braunfeld, M.B., Sedat, J.W, and Agard, DA. "An improved strategy for automated electron microscopic tomography," J. Struct. Biol., (2004) 147(2):91-101 (html or pdf)

Moritz, M., Braunfeld, M.B., Alberts, B.M., Agard, D.A. (2001) Reconstitution of centrosome microtubule nucleation in Drosophila. Methods Cell Biol. (2001) 67:141-8.

Moritz M, Braunfeld MB, Guenebaut V, Heuser J, Agard DA. "Structure of the gamma-tubulin ring complex: a template for microtubule nucleation," Nat Cell Biol. 2000, 2(6), 365-70 (Abstract).

Moritz M, Braunfeld MB, Sedat JW, Alberts B, Agard DA. "Microtubule nucleation by gamma-tubulin-containing rings in the centrosome," Nature, 1995, 378(6557), 638-40.

Moritz M, Braunfeld MB, Fung JC, Sedat JW, Alberts BM, Agard DA."Three-dimensional structural characterization of centrosomes from early Drosophila embryos," J Cell Biol. 1995, 130(5), 1149-59 (Abstract)

Braunfeld MB, Koster AJ, Sedat JW, Agard DA. "Cryo automated electron tomography: towards high-resolution reconstructions of plastic-embedded structures," J Microsc. 1994, 174 ( Pt 2), 75-84.

Belmont AS, Braunfeld MB, Sedat JW, Agard DA. "Large-scale chromatin structural domains within mitotic and interphase chromosomes in vivo and in vitro," Chromosoma. 1989, 98(2), 129-43.

McKinley MP, Braunfeld MB, Bellinger CG, Prusiner SB. "Molecular characteristics of prion rods purified from scrapie-infected hamster brains," J Infect Dis., 1986, 154(1), 110-20.
Meyer RK, McKinley MP, Bowman KA, Braunfeld MB, Barry RA, Prusiner SB. "Separation and properties of cellular and scrapie prion proteins," Proc Natl Acad Sci U S A., 1986, 83(8), 2310-4.

DeArmond SJ, McKinley MP, Barry RA, Braunfeld MB, McColloch JR, Prusiner SB. "Identification of prion amyloid filaments in scrapie-infected brain," Cell, 1985, 41(1), 221-35.