Robert Fletterick, PhD

Professor
Biochemistry and BIophysics
[email protected]
+1 415 476-2829

Robert Fletterick is in the Department of Biochemistry at the University of California, San Francisco where he has served as Chair and Vice Chair.  He is also jointly appointed with the Departments of Pharmaceutical Chemistry and Cellular and Molecular Pharmacology at UCSF.  Robert is a world-renowned researcher in the area of structural biology.  His laboratory has determined high-resolution X-ray crystal structures of nuclear receptors, kinesin molecular motors, clathrin, and many enzymes.  He is known for his work on constructing engineered proteins with new function. For about ten years from 1975-1985, the structures of glycogen phosphorylase determined in his lab were record holders. Glycogen phosphorylase, which controls glucose metabolism but knowing when to degrade glycogen to feed the glycolytic pathway, was the largest molecule imaged by X-ray crystallography. Robert Fletterick’s laboratory solved the first structure of a nuclear receptor bound to its hormone and the first structure of the molecular motor kinesin found in nerve cells, showing it related to the well known myosin motor protein of muscle. Presently his lab studies hormone receptors that regulate embryogenesis, steroid metabolism and prostate development and cancer.
Robert received his Ph.D. from Cornell University and did his postdoctoral research in molecular biophysics in the laboratory of Dr. Thomas Steitz at Yale University.  Before settling at UCSF, Robert was a professor of Biochemistry at the University of Alberta, and a founding member of the MRC Group on Protein Structure and Function. He has coauthored over two hundred eighty basic science papers.

Dr. Fletterick was elected to the National Academy of Sciences, April 2010.

Publications: 

Covalent Modification and Regulation of the Nuclear Receptor Nurr1 by a Dopamine Metabolite.

Cell chemical biology

Bruning JM, Wang Y, Oltrabella F, Tian B, Kholodar SA, Liu H, Bhattacharya P, Guo S, Holton JM, Fletterick RJ, Jacobson MP, England PM

LRH-1 mitigates intestinal inflammatory disease by maintaining epithelial homeostasis and cell survival.

Nature communications

Bayrer JR, Wang H, Nattiv R, Suzawa M, Escusa HS, Fletterick RJ, Klein OD, Moore DD, Ingraham HA

Development of 5N-Bicalutamide, a High-Affinity Reversible Covalent Antiandrogen.

ACS chemical biology

de Jesus Cortez F, Nguyen P, Truillet C, Tian B, Kuchenbecker KM, Evans MJ, Webb P, Jacobson MP, Fletterick RJ, England PM

NR5A2 discovering compounds that block tumor growth in PDAC.

Journal of surgical oncology

Fletterick R

Disulfide-Trapping Identifies a New, Effective Chemical Probe for Activating the Nuclear Receptor Human LRH-1 (NR5A2).

PloS one

de Jesus Cortez F, Suzawa M, Irvy S, Bruning JM, Sablin E, Jacobson MP, Fletterick RJ, Ingraham HA, England PM

Dlx5 Homeodomain:DNA Complex: Structure, Binding and Effect of Mutations Related to Split Hand and Foot Malformation Syndrome.

Journal of molecular biology

Proudfoot A, Axelrod HL, Geralt M, Fletterick RJ, Yumoto F, Deacon AM, Elsliger MA, Wilson IA, Wüthrich K, Serrano P

A gene-expression screen identifies a non-toxic sumoylation inhibitor that mimics SUMO-less human LRH-1 in liver.

eLife

Suzawa M, Miranda DA, Ramos KA, Ang KK, Faivre EJ, Wilson CG, Caboni L, Arkin MR, Kim YS, Fletterick RJ, Diaz A, Schneekloth JS, Ingraham HA

Structure of Liver Receptor Homolog-1 (NR5A2) with PIP3 hormone bound in the ligand binding pocket.

Journal of structural biology

Sablin EP, Blind RD, Uthayaruban R, Chiu HJ, Deacon AM, Das D, Ingraham HA, Fletterick RJ

The FKBP52 Cochaperone Acts in Synergy with ß-Catenin to Potentiate Androgen Receptor Signaling.

PloS one

Storer Samaniego C, Suh JH, Chattopadhyay A, Olivares K, Guy N, Sivils JC, Dey P, Yumoto F, Fletterick RJ, Strom AM, Gustafsson JÅ, Webb P, Cox MB

Structure-based discovery of NANOG variant with enhanced properties to promote self-renewal and reprogramming of pluripotent stem cells.

Proceedings of the National Academy of Sciences of the United States of America

Hayashi Y, Caboni L, Das D, Yumoto F, Clayton T, Deller MC, Nguyen P, Farr CL, Chiu HJ, Miller MD, Elsliger MA, Deacon AM, Godzik A, Lesley SA, Tomoda K, Conklin BR, Wilson IA, Yamanaka S, Fletterick RJ

Histone demethylase KDM5A is regulated by its reader domain through a positive-feedback mechanism.

Nature communications

Torres IO, Kuchenbecker KM, Nnadi CI, Fletterick RJ, Kelly MJ, Fujimori DG

Silencing LRH-1 in colon cancer cell lines impairs proliferation and alters gene expression programs.

Proceedings of the National Academy of Sciences of the United States of America

Bayrer JR, Mukkamala S, Sablin EP, Webb P, Fletterick RJ

Advances in our structural understanding of orphan nuclear receptors.

Trends in biochemical sciences

Gallastegui N, Mackinnon JA, Fletterick RJ, Estébanez-Perpiñá E

The signaling phospholipid PIP3 creates a new interaction surface on the nuclear receptor SF-1.

Proceedings of the National Academy of Sciences of the United States of America

Blind RD, Sablin EP, Kuchenbecker KM, Chiu HJ, Deacon AM, Das D, Fletterick RJ, Ingraham HA

Computer modeling of a newly identified THRB gene mutation (S350L) associated with resistance to thyroid hormone in three unrelated patients.

Thyroid : official journal of the American Thyroid Association

Nagavalli S, Fletterick RJ, Ford G, Boston BA, Gianoukakis AG

The human orphan nuclear receptor tailless (TLX, NR2E1) is druggable.

PloS one

Benod C, Villagomez R, Filgueira CS, Hwang PK, Leonard PG, Poncet-Montange G, Rajagopalan S, Fletterick RJ, Gustafsson JÅ, Webb P

Structural and functional insight into TAF1-TAF7, a subcomplex of transcription factor II D.

Proceedings of the National Academy of Sciences of the United States of America

Bhattacharya S, Lou X, Hwang P, Rajashankar KR, Wang X, Gustafsson JÅ, Fletterick RJ, Jacobson RH, Webb P

Incidental identification of a thyroid hormone receptor beta (THRB) gene variant in a family with autoimmune thyroid disease.

Thyroid : official journal of the American Thyroid Association

Larsen CC, Dumitrescu A, Guerra-Argüero LM, Gállego-Suárez C, Vazquez-Mellado A, Vinogradova M, Fletterick R, Refetoff S, Weiss RE

Plant Kinesin-Like Calmodulin Binding Protein Employs Its Regulatory Domain for Dimerization.

PloS one

Vinogradova MV, Malanina GG, Waitzman JS, Rice SE, Fletterick RJ

Structure-based discovery of antagonists of nuclear receptor LRH-1.

The Journal of biological chemistry

Benod C, Carlsson J, Uthayaruban R, Hwang P, Irwin JJ, Doak AK, Shoichet BK, Sablin EP, Fletterick RJ

Structure of a novel winged-helix like domain from human NFRKB protein.

PloS one

Kumar A, Möcklinghoff S, Yumoto F, Jaroszewski L, Farr CL, Grzechnik A, Nguyen P, Weichenberger CX, Chiu HJ, Klock HE, Elsliger MA, Deacon AM, Godzik A, Lesley SA, Conklin BR, Fletterick RJ, Wilson IA

Structural basis of coactivation of liver receptor homolog-1 by ß-catenin.

Proceedings of the National Academy of Sciences of the United States of America

Yumoto F, Nguyen P, Sablin EP, Baxter JD, Webb P, Fletterick RJ

Nuclear receptor liver receptor homologue 1 (LRH-1) regulates pancreatic cancer cell growth and proliferation.

Proceedings of the National Academy of Sciences of the United States of America

Benod C, Vinogradova MV, Jouravel N, Kim GE, Fletterick RJ, Sablin EP

A conserved surface on the ligand binding domain of nuclear receptors for allosteric control.

Molecular and cellular endocrinology

Buzón V, Carbó LR, Estruch SB, Fletterick RJ, Estébanez-Perpiñá E

Targeting the regulation of androgen receptor signaling by the heat shock protein 90 cochaperone FKBP52 in prostate cancer cells.

Proceedings of the National Academy of Sciences of the United States of America

De Leon JT, Iwai A, Feau C, Garcia Y, Balsiger HA, Storer CL, Suro RM, Garza KM, Lee S, Kim YS, Chen Y, Ning YM, Riggs DL, Fletterick RJ, Guy RK, Trepel JB, Neckers LM, Cox MB

Decoration of microtubules in solution by the kinesin-14, Ncd.

Acta crystallographica. Section D, Biological crystallography

Hjelm RP, Stone DB, Fletterick RJ, Mendelson RA

Conformation switching of clathrin light chain regulates clathrin lattice assembly.

Developmental cell

Wilbur JD, Hwang PK, Ybe JA, Lane M, Sellers BD, Jacobson MP, Fletterick RJ, Brodsky FM

Accommodation of structural rearrangements in the huntingtin-interacting protein 1 coiled-coil domain.

Acta crystallographica. Section D, Biological crystallography

Wilbur JD, Hwang PK, Brodsky FM, Fletterick RJ

Structure of the complex of a mitotic kinesin with its calcium binding regulator.

Proceedings of the National Academy of Sciences of the United States of America

Vinogradova MV, Malanina GG, Reddy AS, Fletterick RJ

Molecular basis for dimer formation of TRbeta variant D355R.

Proteins

Jouravel N, Sablin E, Togashi M, Baxter JD, Webb P, Fletterick RJ

The structure of corepressor Dax-1 bound to its target nuclear receptor LRH-1.

Proceedings of the National Academy of Sciences of the United States of America

Sablin EP, Woods A, Krylova IN, Hwang P, Ingraham HA, Fletterick RJ

Structure of SF-1 bound by different phospholipids: evidence for regulatory ligands.

Molecular endocrinology (Baltimore, Md.)

Sablin EP, Blind RD, Krylova IN, Ingraham JG, Cai F, Williams JD, Fletterick RJ, Ingraham HA

6-Azido-7-nitro-1,4-dihydroquinoxaline-2,3-dione (ANQX) forms an irreversible bond to the active site of the GluR2 AMPA receptor.

Journal of medicinal chemistry

Cruz LA, Estébanez-Perpiñá E, Pfaff S, Borngraeber S, Bao N, Blethrow J, Fletterick RJ, England PM

Actin binding by Hip1 (huntingtin-interacting protein 1) and Hip1R (Hip1-related protein) is regulated by clathrin light chain.

The Journal of biological chemistry

Wilbur JD, Chen CY, Manalo V, Hwang PK, Fletterick RJ, Brodsky FM

Structural dynamics of the microtubule binding and regulatory elements in the kinesin-like calmodulin binding protein.

Journal of structural biology

Vinogradova MV, Malanina GG, Reddy VS, Reddy AS, Fletterick RJ

Interaction between the androgen receptor and a segment of its corepressor SHP.

Acta crystallographica. Section D, Biological crystallography

Jouravel N, Sablin E, Arnold LA, Guy RK, Fletterick RJ

Inhibitors of the interaction of a thyroid hormone receptor and coactivators: preliminary structure-activity relationships.

Journal of medicinal chemistry

Arnold LA, Kosinski A, Estébanez-Perpiñá E, Fletterick RJ, Guy RK

A surface on the androgen receptor that allosterically regulates coactivator binding.

Proceedings of the National Academy of Sciences of the United States of America

Estébanez-Perpiñá E, Arnold LA, Arnold AA, Nguyen P, Rodrigues ED, Mar E, Bateman R, Pallai P, Shokat KM, Baxter JD, Guy RK, Webb P, Fletterick RJ

Perspectives on designs of antiandrogens for prostate cancer.

Expert opinion on drug discovery

Estébanez-Perpiñá E, Jouravel N, Fletterick RJ

Structural insight into the mode of action of a direct inhibitor of coregulator binding to the thyroid hormone receptor.

Molecular endocrinology (Baltimore, Md.)

Estébanez-Perpiñá E, Arnold LA, Jouravel N, Togashi M, Blethrow J, Mar E, Nguyen P, Phillips KJ, Baxter JD, Webb P, Guy RK, Fletterick RJ

The structure of chagasin in complex with a cysteine protease clarifies the binding mode and evolution of an inhibitor family.

Structure (London, England : 1993)

Wang SX, Pandey KC, Scharfstein J, Whisstock J, Huang RK, Jacobelli J, Fletterick RJ, Rosenthal PJ, Abrahamson M, Brinen LS, Rossi A, Sali A, McKerrow JH

Ca ion and the troponin switch.

Advances in experimental medicine and biology

Vinogradova MV, Stone DB, Malanina GG, Mendelson RA, Fletterick RJ

Structural basis for unique mechanisms of folding and hemoglobin binding by a malarial protease.

Proceedings of the National Academy of Sciences of the United States of America

Wang SX, Pandey KC, Somoza JR, Sijwali PS, Kortemme T, Brinen LS, Fletterick RJ, Rosenthal PJ, McKerrow JH

Directed evolution of an anti-prion protein scFv fragment to an affinity of 1 pM and its structural interpretation.

Journal of molecular biology

Luginbühl B, Kanyo Z, Jones RM, Fletterick RJ, Prusiner SB, Cohen FE, Williamson RA, Burton DR, Plückthun A

A high-throughput screening method to identify small molecule inhibitors of thyroid hormone receptor coactivator binding.

Science's STKE : signal transduction knowledge environment

Arnold LA, Estébanez-Perpiñá E, Togashi M, Shelat A, Ocasio CA, McReynolds AC, Nguyen P, Baxter JD, Fletterick RJ, Webb P, Guy RK

An atomic model of the thin filament in the relaxed and Ca2+-activated states.

Journal of molecular biology

Pirani A, Vinogradova MV, Curmi PM, King WA, Fletterick RJ, Craig R, Tobacman LS, Xu C, Hatch V, Lehman W

Dynamics of the C-terminal region of TnI in the troponin complex in solution.

Biophysical journal

Blumenschein TM, Stone DB, Fletterick RJ, Mendelson RA, Sykes BD

Discovery of small molecule inhibitors of the interaction of the thyroid hormone receptor with transcriptional coregulators.

The Journal of biological chemistry

Arnold LA, Estébanez-Perpiñá E, Togashi M, Jouravel N, Shelat A, McReynolds AC, Mar E, Nguyen P, Baxter JD, Fletterick RJ, Webb P, Guy RK

Calcium-dependent changes in the flexibility of the regulatory domain of troponin C in the troponin complex.

The Journal of biological chemistry

Blumenschein TM, Stone DB, Fletterick RJ, Mendelson RA, Sykes BD

Ca(2+)-regulated structural changes in troponin.

Proceedings of the National Academy of Sciences of the United States of America

Vinogradova MV, Stone DB, Malanina GG, Karatzaferi C, Cooke R, Mendelson RA, Fletterick RJ

Conformational adaptation of nuclear receptor ligand binding domains to agonists: potential for novel approaches to ligand design.

The Journal of steroid biochemistry and molecular biology

Togashi M, Borngraeber S, Sandler B, Fletterick RJ, Webb P, Baxter JD

Structural analyses reveal phosphatidyl inositols as ligands for the NR5 orphan receptors SF-1 and LRH-1.

Cell

Krylova IN, Sablin EP, Moore J, Xu RX, Waitt GM, MacKay JA, Juzumiene D, Bynum JM, Madauss K, Montana V, Lebedeva L, Suzawa M, Williams JD, Williams SP, Guy RK, Thornton JW, Fletterick RJ, Willson TM, Ingraham HA

The molecular mechanisms of coactivator utilization in ligand-dependent transactivation by the androgen receptor.

The Journal of biological chemistry

Estébanez-Perpiñá E, Moore JM, Mar E, Delgado-Rodrigues E, Nguyen P, Baxter JD, Buehrer BM, Webb P, Fletterick RJ, Guy RK

Thyroxine-thyroid hormone receptor interactions.

The Journal of biological chemistry

Sandler B, Webb P, Apriletti JW, Huber BR, Togashi M, Cunha Lima ST, Juric S, Nilsson S, Wagner R, Fletterick RJ, Baxter JD

Recognition and accommodation at the androgen receptor coactivator binding interface.

PLoS biology

Hur E, Pfaff SJ, Payne ES, Grøn H, Buehrer BM, Fletterick RJ

Structure of a kinesin microtubule depolymerization machine.

The EMBO journal

Shipley K, Hekmat-Nejad M, Turner J, Moores C, Anderson R, Milligan R, Sakowicz R, Fletterick R

Crystal structure of kinesin regulated by Ca(2+)-calmodulin.

The Journal of biological chemistry

Vinogradova MV, Reddy VS, Reddy AS, Sablin EP, Fletterick RJ

Coordination between motor domains in processive kinesins.

The Journal of biological chemistry

Sablin EP, Fletterick RJ

Ligand selectivity by seeking hydrophobicity in thyroid hormone receptor.

Proceedings of the National Academy of Sciences of the United States of America

Borngraeber S, Budny MJ, Chiellini G, Cunha-Lima ST, Togashi M, Webb P, Baxter JD, Scanlan TS, Fletterick RJ

The extended interactions and Gla domain of blood coagulation factor Xa.

Biochemistry

Wang SX, Hur E, Sousa CA, Brinen L, Slivka EJ, Fletterick RJ

The oligomeric structure of human granzyme A is a determinant of its extended substrate specificity.

Nature structural biology

Bell JK, Goetz DH, Mahrus S, Harris JL, Fletterick RJ, Craik CS

Structural basis for ligand-independent activation of the orphan nuclear receptor LRH-1.

Molecular cell

Sablin EP, Krylova IN, Fletterick RJ, Ingraham HA

Two resistance to thyroid hormone mutants with impaired hormone binding.

Molecular endocrinology (Baltimore, Md.)

Huber BR, Sandler B, West BL, Cunha Lima ST, Nguyen HT, Apriletti JW, Baxter JD, Fletterick RJ

Thyroid hormone receptor-beta mutations conferring hormone resistance and reduced corepressor release exhibit decreased stability in the N-terminal ligand-binding domain.

Molecular endocrinology (Baltimore, Md.)

Huber BR, Desclozeaux M, West BL, Cunha-Lima ST, Nguyen HT, Baxter JD, Ingraham HA, Fletterick RJ

Design of thyroid hormone receptor antagonists from first principles.

The Journal of steroid biochemistry and molecular biology

Webb P, Nguyen NH, Chiellini G, Yoshihara HA, Cunha Lima ST, Apriletti JW, Ribeiro RC, Marimuthu A, West BL, Goede P, Mellstrom K, Nilsson S, Kushner PJ, Fletterick RJ, Scanlan TS, Baxter JD

Two conformations in the human kinesin power stroke defined by X-ray crystallography and EPR spectroscopy.

Nature structural biology

Sindelar CV, Budny MJ, Rice S, Naber N, Fletterick R, Cooke R

Phosphorylation and intramolecular stabilization of the ligand binding domain in the nuclear receptor steroidogenic factor 1.

Molecular and cellular biology

Desclozeaux M, Krylova IN, Horn F, Fletterick RJ, Ingraham HA

Structure of an F-actin trimer disrupted by gelsolin and implications for the mechanism of severing.

The Journal of biological chemistry

Dawson JF, Sablin EP, Spudich JA, Fletterick RJ

How does ATP hydrolysis control actin's associations?

Proceedings of the National Academy of Sciences of the United States of America

Sablin EP, Dawson JF, VanLoock MS, Spudich JA, Egelman EH, Fletterick RJ

Structure-activity analysis of the purine binding site of human liver glycogen phosphorylase.

Chemistry & biology

Ekstrom JL, Pauly TA, Carty MD, Soeller WC, Culp J, Danley DE, Hoover DJ, Treadway JL, Gibbs EM, Fletterick RJ, Day YS, Myszka DG, Rath VL

Computer-assisted mutagenesis of ecotin to engineer its secondary binding site for urokinase inhibition.

The Journal of biological chemistry

Laboissière MC, Young MM, Pinho RG, Todd S, Fletterick RJ, Kuntz I, Craik CS

Structure-based design and synthesis of a thyroid hormone receptor (TR) antagonist.

Endocrinology

Baxter JD, Goede P, Apriletti JW, West BL, Feng W, Mellstrom K, Fletterick RJ, Wagner RL, Kushner PJ, Ribeiro RC, Webb P, Scanlan TS, Nilsson S

TR surfaces and conformations required to bind nuclear receptor corepressor.

Molecular endocrinology (Baltimore, Md.)

Marimuthu A, Feng W, Tagami T, Nguyen H, Jameson JL, Fletterick RJ, Baxter JD, West BL

Nucleotide switches in molecular motors: structural analysis of kinesins and myosins.

Current opinion in structural biology

Sablin EP, Fletterick RJ

Molecular heterogeneity of myophosphorylase deficiency (McArdle's disease): a genotype-phenotype correlation study.

Annals of neurology

Martín MA, Rubio JC, Buchbinder J, Fernández-Hojas R, del Hoyo P, Teijeira S, Gámez J, Navarro C, Fernández JM, Cabello A, Campos Y, Cervera C, Culebras JM, Andreu AL, Fletterick R, Arenas J

Switch-based mechanism of kinesin motors.

Nature

Kikkawa M, Sablin EP, Okada Y, Yajima H, Fletterick RJ, Hirokawa N

The role of ecotin dimerization in protease inhibition.

Journal of molecular biology

Eggers CT, Wang SX, Fletterick RJ, Craik CS

Crystal structure of the mitotic spindle kinesin Eg5 reveals a novel conformation of the neck-linker.

The Journal of biological chemistry

Turner J, Anderson R, Guo J, Beraud C, Fletterick R, Sakowicz R

Hormone selectivity in thyroid hormone receptors.

Molecular endocrinology (Baltimore, Md.)

Wagner RL, Huber BR, Shiau AK, Kelly A, Cunha Lima ST, Scanlan TS, Apriletti JW, Baxter JD, West BL, Fletterick RJ

Definition of the surface in the thyroid hormone receptor ligand binding domain for association as homodimers and heterodimers with retinoid X receptor.

The Journal of biological chemistry

Ribeiro RC, Feng W, Wagner RL, Costa CH, Pereira AC, Apriletti JW, Fletterick RJ, Baxter JD

Selective modulation of thyroid hormone receptor action.

The Journal of steroid biochemistry and molecular biology

Baxter JD, Dillmann WH, West BL, Huber R, Furlow JD, Fletterick RJ, Webb P, Apriletti JW, Scanlan TS

Structural relationships among regulated and unregulated phosphorylases.

Annual review of biophysics and biomolecular structure

Buchbinder JL, Rath VL, Fletterick RJ

Three-dimensional structural analysis of fibronectin heparin-binding domain mutations.

Journal of cellular biochemistry. Supplement

Kapila Y, Doan D, Tafolla E, Fletterick R

A target within the target: probing cruzain's P1' site to define structural determinants for the Chagas' disease protease.

Structure (London, England : 1993)

Brinen LS, Hansell E, Cheng J, Roush WR, McKerrow JH, Fletterick RJ

Compromise and accommodation in ecotin, a dimeric macromolecular inhibitor of serine proteases.

Journal of molecular biology

Gillmor SA, Takeuchi T, Yang SQ, Craik CS, Fletterick RJ

Searching for kinesin's mechanical amplifier.

Philosophical transactions of the Royal Society of London. Series B, Biological sciences

Vale RD, Case R, Sablin E, Hart C, Fletterick R

Shape and specificity in mammalian 15-lipoxygenase active site. The functional interplay of sequence determinants for the reaction specificity.

The Journal of biological chemistry

Borngräber S, Browner M, Gillmor S, Gerth C, Anton M, Fletterick R, Kühn H

Visualizing a new binding site of ncd-motor domain on tubulin.

Journal of structural biology

Han Y, Sablin EP, Nogales E, Fletterick RJ, Downing KH

Antibody binding defines a structure for an epitope that participates in the PrPC-->PrPSc conformational change.

Journal of molecular biology

Kanyo ZF, Pan KM, Williamson RA, Burton DR, Prusiner SB, Fletterick RJ, Cohen FE

Three-dimensional model of the ligand binding domain of the nuclear receptor for 1alpha,25-dihydroxy-vitamin D(3).

Journal of cellular biochemistry

Norman AW, Adams D, Collins ED, Okamura WH, Fletterick RJ

Clathrin self-assembly is mediated by a tandemly repeated superhelix.

Nature

Ybe JA, Brodsky FM, Hofmann K, Lin K, Liu SH, Chen L, Earnest TN, Fletterick RJ, Hwang PK

Nuclear-receptor ligands and ligand-binding domains.

Annual review of biochemistry

Weatherman RV, Fletterick RJ, Scanlan TS

Molecular and structural biology of thyroid hormone receptors.

Clinical and experimental pharmacology & physiology. Supplement

Apriletti JW, Ribeiro RC, Wagner RL, Feng W, Webb P, Kushner PJ, West BL, Nilsson S, Scanlan TS, Fletterick RJ, Baxter JD

Structure and specificity of nuclear receptor-coactivator interactions.

Genes & development

Darimont BD, Wagner RL, Apriletti JW, Stallcup MR, Kushner PJ, Baxter JD, Fletterick RJ, Yamamoto KR

The case for a common ancestor: kinesin and myosin motor proteins and G proteins.

Journal of muscle research and cell motility

Kull FJ, Vale RD, Fletterick RJ

A 1.4 A crystal structure for the hypoxanthine phosphoribosyltransferase of Trypanosoma cruzi.

Biochemistry

Focia PJ, Craig SP, Nieves-Alicea R, Fletterick RJ, Eakin AE

Direction determination in the minus-end-directed kinesin motor ncd.

Nature

Sablin EP, Case RB, Dai SC, Hart CL, Ruby A, Vale RD, Fletterick RJ

A comparison of the crystallographic structures of two catalytic antibodies with esterase activity.

Journal of molecular biology

Buchbinder JL, Stephenson RC, Scanlan TS, Fletterick RJ

A role for helix 3 of the TRbeta ligand-binding domain in coactivator recruitment identified by characterization of a third cluster of mutations in resistance to thyroid hormone.

The EMBO journal

Collingwood TN, Wagner R, Matthews CH, Clifton-Bligh RJ, Gurnell M, Rajanayagam O, Agostini M, Fletterick RJ, Beck-Peccoz P, Reinhardt W, Binder G, Ranke MB, Hermus A, Hesch RD, Lazarus J, Newrick P, Parfitt V, Raggatt P, de Zegher F, Chatterjee VK

Is the tubulin/FtsZ fold related to the G-protein fold?

Trends in cell biology

Kull FJ, Fletterick RJ

Ecotin: a serine protease inhibitor with two distinct and interacting binding sites.

Journal of molecular biology

Yang SQ, Wang CI, Gillmor SA, Fletterick RJ, Craik CS

Hormone-dependent coactivator binding to a hydrophobic cleft on nuclear receptors.

Science (New York, N.Y.)

Feng W, Ribeiro RC, Wagner RL, Nguyen H, Apriletti JW, Fletterick RJ, Baxter JD, Kushner PJ, West BL

A novel TR beta mutation (R383H) in resistance to thyroid hormone syndrome predominantly impairs corepressor release and negative transcriptional regulation.

Molecular endocrinology (Baltimore, Md.)

Clifton-Bligh RJ, de Zegher F, Wagner RL, Collingwood TN, Francois I, Van Helvoirt M, Fletterick RJ, Chatterjee VK

Rational design of novel antimicrobials: blocking purine salvage in a parasitic protozoan.

Biochemistry

Somoza JR, Skillman AG, Munagala NR, Oshiro CM, Knegtel RM, Mpoke S, Fletterick RJ, Kuntz ID, Wang CC

Biochemical characterization and crystallographic structure of an Escherichia coli protein from the phosphotriesterase gene family.

Biochemistry

Buchbinder JL, Stephenson RC, Dresser MJ, Pitera JW, Scanlan TS, Fletterick RJ

DAX1 mutations map to putative structural domains in a deduced three-dimensional model.

American journal of human genetics

Zhang YH, Guo W, Wagner RL, Huang BL, McCabe L, Vilain E, Burris TP, Anyane-Yeboa K, Burghes AH, Chitayat D, Chudley AE, Genel M, Gertner JM, Klingensmith GJ, Levine SN, Nakamoto J, New MI, Pagon RA, Pappas JG, Quigley CA, Rosenthal IM, Baxter JD, Fletterick RJ, McCabe ER

X-ray crystallographic and functional studies of thyroid hormone receptor.

The Journal of steroid biochemistry and molecular biology

Ribeiro RC, Apriletti JW, Wagner RL, Feng W, Kushner PJ, Nilsson S, Scanlan TS, West BL, Fletterick RJ, Baxter JD

Mechanisms of thyroid hormone action: insights from X-ray crystallographic and functional studies.

Recent progress in hormone research

Ribeiro RC, Apriletti JW, Wagner RL, West BL, Feng W, Huber R, Kushner PJ, Nilsson S, Scanlan T, Fletterick RJ, Schaufele F, Baxter JD

The structure of mammalian 15-lipoxygenase reveals similarity to the lipases and the determinants of substrate specificity.

Nature structural biology

Gillmor SA, Villaseñor A, Fletterick R, Sigal E, Browner MF

Distinct phosphorylation signals converge at the catalytic center in glycogen phosphorylases.

Structure (London, England : 1993)

Lin K, Hwang PK, Fletterick RJ

Crystal structure of Tritrichomonas foetus inosine-5'-monophosphate dehydrogenase and the enzyme-product complex.

Biochemistry

Whitby FG, Luecke H, Kuhn P, Somoza JR, Huete-Perez JA, Phillips JD, Hill CP, Fletterick RJ, Wang CC

Structural determinants of specificity in the cysteine protease cruzain.

Protein science : a publication of the Protein Society

Gillmor SA, Craik CS, Fletterick RJ

A model for the microtubule-Ncd motor protein complex obtained by cryo-electron microscopy and image analysis.

Cell

Sosa H, Dias DP, Hoenger A, Whittaker M, Wilson-Kubalek E, Sablin E, Fletterick RJ, Vale RD, Milligan RA

A natural transactivation mutation in the thyroid hormone beta receptor: impaired interaction with putative transcriptional mediators.

Proceedings of the National Academy of Sciences of the United States of America

Collingwood TN, Rajanayagam O, Adams M, Wagner R, Cavaillès V, Kalkhoven E, Matthews C, Nystrom E, Stenlof K, Lindstedt G, Tisell L, Fletterick RJ, Parker MG, Chatterjee VK

The design plan of kinesin motors.

Annual review of cell and developmental biology

Vale RD, Fletterick RJ

A protein phosphorylation switch at the conserved allosteric site in GP.

Science (New York, N.Y.)

Lin K, Rath VL, Dai SC, Fletterick RJ, Hwang PK

Structure-based design of parasitic protease inhibitors.

Bioorganic & medicinal chemistry

Li R, Chen X, Gong B, Selzer PM, Li Z, Davidson E, Kurzban G, Miller RE, Nuzum EO, McKerrow JH, Fletterick RJ, Gillmor SA, Craik CS, Kuntz ID, Cohen FE, Kenyon GL

Delocalizing trypsin specificity with metal activation.

Biochemistry

Willett WS, Brinen LS, Fletterick RJ, Craik CS

X-ray structures of a designed binding site in trypsin show metal-dependent geometry.

Biochemistry

Brinen LS, Willett WS, Craik CS, Fletterick RJ

High-level expression and characterization of a purified 142-residue polypeptide of the prion protein.

Biochemistry

Mehlhorn I, Groth D, Stöckel J, Moffat B, Reilly D, Yansura D, Willett WS, Baldwin M, Fletterick R, Cohen FE, Vandlen R, Henner D, Prusiner SB

The evolution of an allosteric site in phosphorylase.

Structure (London, England : 1993)

Rath VL, Lin K, Hwang PK, Fletterick RJ

Crystal structure of the motor domain of the kinesin-related motor ncd.

Nature

Sablin EP, Kull FJ, Cooke R, Vale RD, Fletterick RJ

A structural role for hormone in the thyroid hormone receptor.

Nature

Wagner RL, Apriletti JW, McGrath ME, West BL, Baxter JD, Fletterick RJ

Mechanism of regulation in yeast glycogen phosphorylase.

The Journal of biological chemistry

Lin K, Hwang PK, Fletterick RJ

Expression, purification, ATPase properties, and microtubule-binding properties of the ncd motor domain.

Biochemistry

Shimizu T, Sablin E, Vale RD, Fletterick R, Pechatnikova E, Taylor EW

X-ray diffraction of scrapie prion rods and PrP peptides.

Journal of molecular biology

Nguyen JT, Inouye H, Baldwin MA, Fletterick RJ, Cohen FE, Prusiner SB, Kirschner DA

When one and one are not two.

Nature structural biology

Fletterick RJ, Bazan JF

Three-dimensional structure of a tubulin-motor-protein complex.

Nature

Hoenger A, Sablin EP, Vale RD, Fletterick RJ, Milligan RA

The molecular biology of thyroid hormone action.

Annals of the New York Academy of Sciences

Ribeiro RC, Apriletti JW, West BL, Wagner RL, Fletterick RJ, Schaufele F, Baxter JD

The crystal structure of cruzain: a therapeutic target for Chagas' disease.

Journal of molecular biology

McGrath ME, Eakin AE, Engel JC, McKerrow JH, Craik CS, Fletterick RJ

Mechanistically different catalytic antibodies obtained from immunization with a single transition-state analog.

Proceedings of the National Academy of Sciences of the United States of America

Guo J, Huang W, Zhou GW, Fletterick RJ, Scanlan TS

Engineered metal regulation of trypsin specificity.

Biochemistry

Willett WS, Gillmor SA, Perona JJ, Fletterick RJ, Craik CS

Structural origins of substrate discrimination in trypsin and chymotrypsin.

Biochemistry

Perona JJ, Hedstrom L, Rutter WJ, Fletterick RJ

Ecotin: lessons on survival in a protease-filled world.

Protein science : a publication of the Protein Society

McGrath ME, Gillmor SA, Fletterick RJ

Parallel evolution in two homologues of phosphorylase.

Nature structural biology

Rath VL, Fletterick RJ

Crystal structure of a catalytic antibody with a serine protease active site.

Science (New York, N.Y.)

Zhou GW, Guo J, Huang W, Fletterick RJ, Scanlan TS

A negative electrostatic determinant mediates the association between the Escherichia coli trp repressor and its operator DNA.

Protein science : a publication of the Protein Society

Guenot J, Fletterick RJ, Kollman PA

Proposed three-dimensional structure for the cellular prion protein.

Proceedings of the National Academy of Sciences of the United States of America

Huang Z, Gabriel JM, Baldwin MA, Fletterick RJ, Prusiner SB, Cohen FE

Identification of the molecular trigger for allosteric activation in glycogen phosphorylase.

Nature structural biology

Browner MF, Hackos D, Fletterick R

Structural clues to prion replication.

Science (New York, N.Y.)

Cohen FE, Pan KM, Huang Z, Baldwin M, Fletterick RJ, Prusiner SB

Deconvoluting serpins.

Nature structural biology

Fletterick RJ, McGrath ME

Spectroscopic characterization of conformational differences between PrPC and PrPSc: an alpha-helix to beta-sheet transition.

Philosophical transactions of the Royal Society of London. Series B, Biological sciences

Baldwin MA, Pan KM, Nguyen J, Huang Z, Groth D, Serban A, Gasset M, Mehlhorn I, Fletterick RJ, Cohen FE

Preliminary crystallographic studies of the ligand-binding domain of the thyroid hormone receptor complexed with triiodothyronine.

Journal of molecular biology

McGrath ME, Wagner RL, Apriletti JW, West BL, Ramalingam V, Baxter JD, Fletterick RJ

Exogenous acetate reconstitutes the enzymatic activity of trypsin Asp189Ser.

Biochemistry

Perona JJ, Hedstrom L, Wagner RL, Rutter WJ, Craik CS, Fletterick RJ

Conversion of alpha-helices into beta-sheets features in the formation of the scrapie prion proteins.

Proceedings of the National Academy of Sciences of the United States of America

Pan KM, Baldwin M, Nguyen J, Gasset M, Serban A, Groth D, Mehlhorn I, Huang Z, Fletterick RJ, Cohen FE

PRISM: application to the solution of two protein structures.

Acta crystallographica. Section D, Biological crystallography

Bystroff C, Baker D, Fletterick RJ, Agard DA

PRISM: topologically constrained phased refinement for macromolecular crystallography.

Acta crystallographica. Section D, Biological crystallography

Baker D, Bystroff C, Fletterick RJ, Agard DA

Locating the catalytic water molecule in serine proteases.

Science (New York, N.Y.)

Perona JJ, Craik CS, Fletterick RJ

Crystal structures of rat anionic trypsin complexed with the protein inhibitors APPI and BPTI.

Journal of molecular biology

Perona JJ, Tsu CA, Craik CS, Fletterick RJ

Relocating a negative charge in the binding pocket of trypsin.

Journal of molecular biology

Perona JJ, Tsu CA, McGrath ME, Craik CS, Fletterick RJ

Production of crystallizable cruzain, the major cysteine protease from Trypanosoma cruzi.

The Journal of biological chemistry

Eakin AE, McGrath ME, McKerrow JH, Fletterick RJ, Craik CS

Structure of an engineered, metal-actuated switch in trypsin.

Biochemistry

McGrath ME, Haymore BL, Summers NL, Craik CS, Fletterick RJ

Perturbation of the secondary structure of the scrapie prion protein under conditions that alter infectivity.

Proceedings of the National Academy of Sciences of the United States of America

Gasset M, Baldwin MA, Fletterick RJ, Prusiner SB

Purification of glycogen phosphorylase isozymes by metal-affinity chromatography.

Journal of chromatography

Luong CB, Browner MF, Fletterick RJ, Haymore BL

Cooperative binding is not required for activation of muscle phosphorylase.

Biochemistry

Browner MF, Hwang PK, Fletterick RJ

Tracking conformational states in allosteric transitions of phosphorylase.

Biochemistry

Browner MF, Fauman EB, Fletterick RJ

The crystal structure of the Bacillus lentus alkaline protease, subtilisin BL, at 1.4 A resolution.

Journal of molecular biology

Goddette DW, Paech C, Yang SS, Mielenz JR, Bystroff C, Wilke ME, Fletterick RJ

Predicted alpha-helical regions of the prion protein when synthesized as peptides form amyloid.

Proceedings of the National Academy of Sciences of the United States of America

Gasset M, Baldwin MA, Lloyd DH, Gabriel JM, Holtzman DM, Cohen F, Fletterick R, Prusiner SB

Purification and crystallization of glycogen phosphorylase from Saccharomyces cerevisiae.

Journal of molecular biology

Rath VL, Hwang PK, Fletterick RJ

Perturbing the polar environment of Asp102 in trypsin: consequences of replacing conserved Ser214.

Biochemistry

McGrath ME, Vásquez JR, Craik CS, Yang AS, Honig B, Fletterick RJ

Engineered metalloregulation in enzymes.

Trends in biochemical sciences

Higaki JN, Fletterick RJ, Craik CS

Physical map location of the new Escherichia coli gene eco, encoding the serine protease inhibitor ecotin.

Journal of bacteriology

Erpel T, Hwang P, Craik CS, Fletterick RJ, McGrath ME

Phosphorylase: a biological transducer.

Trends in biochemical sciences

Browner MF, Fletterick RJ

Structural basis for the activation of glycogen phosphorylase b by adenosine monophosphate.

Science (New York, N.Y.)

Sprang SR, Withers SG, Goldsmith EJ, Fletterick RJ, Madsen NB

Expression of the protease inhibitor ecotin and its co-crystallization with trypsin.

Journal of molecular biology

McGrath ME, Erpel T, Browner MF, Fletterick RJ

An engineered liver glycogen phosphorylase with AMP allosteric activation.

The Journal of biological chemistry

Coats WS, Browner MF, Fletterick RJ, Newgard CB

The sequence and reactive site of ecotin. A general inhibitor of pancreatic serine proteases from Escherichia coli.

The Journal of biological chemistry

McGrath ME, Hines WM, Sakanari JA, Fletterick RJ, Craik CS

Temperature-sensitive production of rabbit muscle glycogen phosphorylase in Escherichia coli.

Protein engineering

Browner MF, Rasor P, Tugendreich S, Fletterick RJ

Hydrogen bonds involving sulfur atoms in proteins.

Proteins

Gregoret LM, Rader SD, Fletterick RJ, Cohen FE

Purification and crystallization of 15-lipoxygenase from rabbit reticulocytes.

Biochemical and biophysical research communications

Sloane DL, Browner MF, Dauter Z, Wilson K, Fletterick RJ, Sigal E

Arginine 127 stabilizes the transition state in carboxypeptidase.

The Journal of biological chemistry

Phillips MA, Fletterick R, Rutter WJ

Evidence that the N-terminal domain of nonstructural protein NS3 from yellow fever virus is a serine protease responsible for site-specific cleavages in the viral polyprotein.

Proceedings of the National Academy of Sciences of the United States of America

Chambers TJ, Weir RC, Grakoui A, McCourt DW, Bazan JF, Fletterick RJ, Rice CM

Rare McArdle disease locus polymorphic site on 11q13 contains CpG sequence.

Human genetics

Lebo RV, Anderson LA, DiMauro S, Lynch E, Hwang P, Fletterick R

Regulation of serine protease activity by an engineered metal switch.

Biochemistry

Higaki JN, Haymore BL, Chen S, Fletterick RJ, Craik CS

Electrical potentials in trypsin isozymes.

Biochemistry

Soman K, Yang AS, Honig B, Fletterick R

Evolution of a bifunctional enzyme: 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase.

Proceedings of the National Academy of Sciences of the United States of America

Bazan JF, Fletterick RJ, Pilkis SJ

Crystal structures of two engineered thiol trypsins.

Biochemistry

McGrath ME, Wilke ME, Higaki JN, Craik CS, Fletterick RJ

Primary structure of rabbit skeletal muscle glycogen synthase deduced from cDNA clones.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Zhang WM, Browner MF, Fletterick RJ, DePaoli-Roach AA, Roach PJ

Characterization of the catalytic residues of the tobacco etch virus 49-kDa proteinase.

Virology

Dougherty WG, Parks TD, Cary SM, Bazan JF, Fletterick RJ

Domain separation in the activation of glycogen phosphorylase a.

Science (New York, N.Y.)

Goldsmith EJ, Sprang SR, Hamlin R, Xuong NH, Fletterick RJ

Molecular analysis of GPH1, the gene encoding glycogen phosphorylase in Saccharomyces cerevisiae.

Molecular and cellular biology

Hwang PK, Tugendreich S, Fletterick RJ

Human muscle glycogen synthase cDNA sequence: a negatively charged protein with an asymmetric charge distribution.

Proceedings of the National Academy of Sciences of the United States of America

Browner MF, Nakano K, Bang AG, Fletterick RJ

Rapid preparation of proteins for crystallization trials.

BioTechniques

McGrath ME, Fletterick RJ, Craik CS

The family of glycogen phosphorylases: structure and function.

Critical reviews in biochemistry and molecular biology

Newgard CB, Hwang PK, Fletterick RJ

McArdle's disease: biochemical and molecular genetic studies.

Annals of neurology

Servidei S, Shanske S, Zeviani M, Lebo R, Fletterick R, DiMauro S

Structural changes in glycogen phosphorylase induced by phosphorylation.

Nature

Sprang SR, Acharya KR, Goldsmith EJ, Stuart DI, Varvill K, Fletterick RJ, Madsen NB, Johnson LN

Viral cysteine proteases are homologous to the trypsin-like family of serine proteases: structural and functional implications.

Proceedings of the National Academy of Sciences of the United States of America

Bazan JF, Fletterick RJ

Studies of specificity and catalysis in trypsin by structural analysis of site-directed mutants.

Critical reviews in biotechnology

Sprang SR, Fletterick RJ, Gráf L, Rutter WJ, Craik CS

The burden of proof in linking AIDS to scrapie.

Nature

Braun MJ, Gonda MA, George DG, Bazan JF, Fletterick RJ, Prusiner SB

Primary sequence and structural analysis of sterol carrier protein 2 from rat liver: homology with immunoglobulins.

The Journal of biological chemistry

Pastuszyn A, Noland BJ, Bazan JF, Fletterick RJ, Scallen TJ

Structure of the nucleotide activation switch in glycogen phosphorylase a.

Science (New York, N.Y.)

Sprang S, Goldsmith E, Fletterick R

The three-dimensional structure of Asn102 mutant of trypsin: role of Asp102 in serine protease catalysis.

Science (New York, N.Y.)

Sprang S, Standing T, Fletterick RJ, Stroud RM, Finer-Moore J, Xuong NH, Hamlin R, Rutter WJ, Craik CS

The polymorphic locus for glycogen storage disease VI (liver glycogen phosphorylase) maps to chromosome 14.

American journal of human genetics

Newgard CB, Fletterick RJ, Anderson LA, Lebo RV

Redesigning trypsin via genetic engineering.

Journal of cellular biochemistry

Craik CS, Roczniak S, Sprang S, Fletterick R, Rutter W

The three-dimensional structure of acarbose bound to glycogen phosphorylase.

The Journal of biological chemistry

Goldsmith EJ, Fletterick RJ, Withers SG

AIDS virus and scrapie protein genes.

Nature

Fernando Bazan J, Fletterick RJ, Prusiner SB

Predicted secondary structure and membrane topology of the scrapie prion protein.

Protein engineering

Bazan JF, Fletterick RJ, McKinley MP, Prusiner SB

Intron/exon structure of the human gene for the muscle isozyme of glycogen phosphorylase.

Proteins

Burke J, Hwang P, Anderson L, Lebo R, Gorin F, Fletterick R

Modeling the biochemical differences between rabbit muscle and human liver phosphorylase.

Proteins

Rath VL, Newgard CB, Sprang SR, Goldsmith EJ, Fletterick RJ

Sequence analysis of the cDNA encoding human liver glycogen phosphorylase reveals tissue-specific codon usage.

Proceedings of the National Academy of Sciences of the United States of America

Newgard CB, Nakano K, Hwang PK, Fletterick RJ

Complete cDNA sequence for rabbit muscle glycogen phosphorylase.

FEBS letters

Nakano K, Hwang PK, Fletterick RJ

Turn prediction in proteins using a pattern-matching approach.

Biochemistry

Cohen FE, Abarbanel RM, Kuntz ID, Fletterick RJ

Structural relationships in glycogen phosphorylases.

Annals of the New York Academy of Sciences

Fletterick RJ, Burke JA, Hwang PK, Nakano K, Newgard CB

Comparative sequence analysis of rat, rabbit, and human muscle glycogen phosphorylase cDNAs.

European journal of biochemistry

Hwang PK, See YP, Vincentini AM, Powers MA, Fletterick RJ, Crerar MM

Redesigning trypsin: alteration of substrate specificity.

Science (New York, N.Y.)

Craik CS, Largman C, Fletcher T, Roczniak S, Barr PJ, Fletterick R, Rutter WJ

Purification and crystallization of bovine liver phosphorylase.

Biochimica et biophysica acta

Hwang P, Stern M, Fletterick RJ

High-resolution chromosome sorting and DNA spot-blot analysis assign McArdle's syndrome to chromosome 11.

Science (New York, N.Y.)

Lebo RV, Gorin F, Fletterick RJ, Kao FT, Cheung MC, Bruce BD, Kan YW

Crystallization of the gene 45 protein from the DNA replication fork of bacteriophage T4.

The Journal of biological chemistry

Thaller C, Alberts B, Goldsmith E, Sprang S, Fletterick R

Secondary structure assignment for alpha/beta proteins by a combinatorial approach.

Biochemistry

Cohen FE, Abarbanel RM, Kuntz ID, Fletterick RJ

Splice junctions: association with variation in protein structure.

Science (New York, N.Y.)

Craik CS, Rutter WJ, Fletterick R

Catalytic site of glycogen phosphorylase: structure of the T state and specificity for alpha-D-glucose.

Biochemistry

Sprang SR, Goldsmith EJ, Fletterick RJ, Withers SG, Madsen NB

Intron-exon splice junctions map at protein surfaces.

Nature

Craik CS, Sprang S, Fletterick R, Rutter WJ

Analysis of an allosteric binding site: the nucleoside inhibitor site of phosphorylase alpha.

Biochemistry

Sprang S, Fletterick R, Stern M, Yang D, Madsen N, Sturtevant J

Structure of maltoheptaose by difference Fourier methods and a model for glycogen.

Journal of molecular biology

Goldsmith E, Sprang S, Fletterick R

Subunit interactions and the allosteric response in phosphorylase.

Biophysical journal

Sprang S, Fletterick RJ

Computer simulation of cellular self-sorting: a topological exchange model.

Journal of theoretical biology

Matela RJ, Fletterick RJ

The structures and related functions of phosphorylase a.

Annual review of biochemistry

Fletterick RJ, Madsen NB

Solvent accessibility properties of complex proteins.

Nature

Sprang S, Yang D, Fletterick RJ

The structure of glycogen phosphorylase alpha at 2.5 A resolution.

Journal of molecular biology

Sprang S, Fletterick RJ

A systematic approach to isomorphous replacement using a series of simple charged mercurials.

Biochemical and biophysical research communications

Withers SG, Yang DS, Madsen NB, Fletterick RJ

A topological exchange model for cell self-sorting.

Journal of theoretical biology

Matela RJ, Fletterick RJ

Allosteric transitions of phosphorylase a and the regulation of glycogen metabolism.

The Journal of biological chemistry

Madsen NB, KasvinskyPJ , Fletterick RJ

Synergistic regulation of phosphorylase a by glucose and caffeine.

The Journal of biological chemistry

Kasvinsky PJ, Shechosky S, Fletterick RJ

The regulation of glycogen phosphorylase alpha by nucleotide derivatives. Kinetic and x-ray crystallographic studies.

The Journal of biological chemistry

Kasvinsky PJ, Madsen NB, Sygusch J, Fletterick RJ

X-ray crystallographic and kinetic studies of oligosaccharide binding to phosphorylase.

The Journal of biological chemistry

Kasvinsky PJ, Madsen NB, Fletterick RJ, Sygusch J

Location of pyridoxal phosphate in glycogen phosphorylase a.

Proceedings of the National Academy of Sciences of the United States of America

Sygusch J, Madsen NB, Kasvinsky PJ, Fletterick RJ

An essential arginine residue in the active-site pocket of glycogen phosporylase.

Canadian journal of biochemistry

Li EC, Fletterick RJ, Sygusch J, Madsen NB

Structure of glycogen phosphorylase a at 3.0 A resolution and its ligand binding sites at 6 A.

The Journal of biological chemistry

Fletterick RJ, Sygusch J, Semple H, Madsen NB

Low-resolution structure of the glycogen phosphorylase alpha monomer and comparison with phosphorylase beta.

Journal of molecular biology

Fletterick RJ, Sygusch J, Murray N, Madsen NB

The structure of a yeast hexokinase monomer and its complexes with substrates at 2.7-A resolution.

Proceedings of the National Academy of Sciences of the United States of America

Fletterick RJ, Bates DJ, Steitz TA

The crystal and molecular structure of L-alanyl-L-alanine.

The Journal of physical chemistry

Fletterick RJ, Tsai C, Hughes RE

The crystal and molecular structure of L-alanyl-L-alanine.

The Journal of physical chemistry

Fletterick RJ, Tsai CC, Hughes RE