eLife

Maximizing CRISPRi efficacy and accessibility with dual-sgRNA libraries and optimal effectors.

eLife

Replogle JM, Bonnar JL, Pogson AN, Liem CR, Maier NK, Ding Y, Russell BJ, Wang X, Leng K, Guna A, Norman TM, Pak RA, Ramos DM, Ward ME, Gilbert LA, Kampmann M, Weissman JS, Jost M

Destabilizers of the thymidylate synthase homodimer accelerate its proteasomal degradation and inhibit cancer growth.

eLife

Costantino L, Ferrari S, Santucci M, Salo-Ahen OMH, Carosati E, Franchini S, Lauriola A, Pozzi C, Trande M, Gozzi G, Saxena P, Cannazza G, Losi L, Cardinale D, Venturelli A, Quotadamo A, Linciano P, Tagliazucchi L, Moschella MG, Guerrini R, Pacifico S, Luciani R, Genovese F, Henrich S, Alboni S, Santarem N, da Silva Cordeiro A, Giovannetti E, Peters GJ, Pinton P, Rimessi A, Cruciani G, Stroud RM, Wade RC, Mangani S, Marverti G, D'Arca D, Ponterini G, Costi MP

Antibacterial potency of type VI amidase effector toxins is dependent on substrate topology and cellular context.

eLife

Radkov A, Sapiro AL, Flores S, Henderson C, Saunders H, Kim R, Massa S, Thompson S, Mateusiak C, Biboy J, Zhao Z, Starita LM, Hatleberg WL, Vollmer W, Russell AB, Simorre JP, Anthony-Cahill S, Brzovic P, Hayes B, Chou S

Ligand binding remodels protein side chain conformational heterogeneity.

eLife

Wankowicz SA, de Oliveira SH, Hogan DW, van den Bedem H, Fraser JS

Directed evolution of the rRNA methylating enzyme Cfr reveals molecular basis of antibiotic resistance.

eLife

Tsai K, Stojkovic V, Noda-Garcia L, Young ID, Myasnikov AG, Kleinman J, Palla A, Floor SN, Frost A, Fraser JS, Tawfik DS, Fujimori DG

Genome-wide CRISPRi screening identifies OCIAD1 as a prohibitin client and regulatory determinant of mitochondrial Complex III assembly in human cells.

eLife

Le Vasseur M, Friedman J, Jost M, Xu J, Yamada J, Kampmann M, Horlbeck MA, Salemi MR, Phinney BS, Weissman JS, Nunnari J

CM1-driven assembly and activation of yeast ?-tubulin small complex underlies microtubule nucleation.

eLife

Brilot AF, Lyon AS, Zelter A, Viswanath S, Maxwell A, MacCoss MJ, Muller EG, Sali A, Davis TN, Agard DA

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